LOCUS       sequence-19899-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, 
            Sabatini DM
  TITLE     mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms 
            define three distinct mTORC2s.
  JOURNAL   Curr Biol. 2006 Sep 19;16(18):1865-70. doi: 
            10.1016/j.cub.2006.08.001. Epub 2006 Aug 17.
  PUBMED    16919458
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2006.08.001"; journalName: "Curr Biol"; date: 
            "2006-09-19- 19"; volume: "16"; issue: "18"; pages: "1865-70"
COMMENT     Sequence Label: mSin1 shRNA #1
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        295..805
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     724..742
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 tggcttttta tcttgtggaa aggacgaaac accgggttgg gactttggta ttagaactcg
       61 agttctaata ccaaagtccc aactttttaa ttctcgacct cgagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct cgagggggtt
      301 ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg
      361 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg
      421 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc
      481 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc
      541 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg
      601 accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg
      661 ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc
      721 gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga
      781 ccgaatcacc gacctctctc cccaggggga tccaccggag cttaccatga ccgagtacaa
      841 gccc
//
