LOCUS       sequence-198998-        5921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5921)
  TITLE     Id1 plasmids
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3 hId1
FEATURES             Location/Qualifiers
     source          1..5921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(47..65)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(48..65)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(85..102)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    91..315
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      361..789
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(448..467)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     658..679
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(798..818)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        803..1132
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      983..1118
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1045..1064
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1199..1993
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1253..1272)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1863..1882
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2167..2288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2204..2223)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2258..2277
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2337..2353)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2337..2353)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2350..2372)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2361..2377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2385..2391,2392..2409,2410..2415))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2430..2451
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2568..2585)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2739..3327)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2819..2838)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3498..4289,4290..4358))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4121..4140
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4359..4463)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4538..4557)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4729..5108
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5109..5312
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5263..5283
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5357..5376
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5357..5375
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttgtgtcgc tgagtcgacc tcgagcatgc atctagaggg ccctattcta tagtgtcacc
       61 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      121 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
      181 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
      241 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
      301 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
      361 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      421 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      481 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      541 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      601 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      661 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      721 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      781 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
      841 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      901 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      961 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     1021 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     1081 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     1141 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     1201 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     1261 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     1321 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     1381 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     1441 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     1501 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     1561 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     1621 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     1681 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     1741 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1801 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1861 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     1921 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1981 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     2041 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     2101 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     2161 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     2221 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     2281 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     2341 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2401 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2461 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2521 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2581 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2641 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2701 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2761 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2821 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2881 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2941 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3001 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3061 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3121 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3181 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3241 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3301 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3361 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3421 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3481 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3541 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3601 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3661 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3721 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3781 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3841 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3901 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3961 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4021 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4081 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4141 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4201 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4261 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4321 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4381 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4441 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     4501 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     4561 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     4621 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     4681 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     4741 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     4801 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     4861 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     4921 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     4981 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     5041 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     5101 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     5161 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     5221 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     5281 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt
     5341 actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg taccgagctc
     5401 ggatccacta gtaacggccg ccagtgtgct ggaattcgat atcaagctta tcgataccgg
     5461 gtcgacatga aagtcgccag tggcagcacc gccaccgccg ccgcgggccc cagctgcgcg
     5521 ctgaaggccg gcaagacagc gagcggtgcg ggcgaggtgg tgcgctgtct gtctgagcag
     5581 agcgtggcca tctcgcgctg cgccgggggc gccggggcgc gcctgcctgc cctgctggac
     5641 gagcagcagg taaacgtgct gctctacgac atgaacggct gttactcacg cctcaaggag
     5701 ctggtgccca ccctgcccca gaaccgcaag gtgagcaagg tggagattct ccagcacgtc
     5761 atcgactaca tcagggacct tcagttggag ctgaactcgg aatccgaagt tggaaccccc
     5821 gggggccgag ggctgccggt ccgggctccg ctcagcaccc tcaacggcga gatcagcgcc
     5881 ctgacggccg aggcggcatg cgttcctgcg gacgatcgca t
//
