LOCUS       sequence-199000-        9545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A single-chain light-controllable dSaCas9 with pdDronpa1 domains for
            hGRIN2B gene editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Zhou XX, Zou X, Chung HK, Gao Y, Liu Y, Qi LS, Lin MZ
  TITLE     A Single-Chain Photoswitchable CRISPR-Cas9 Architecture for 
            Light-Inducible Gene Editing and Transcription.
  JOURNAL   ACS Chem Biol. 2017 Sep 29. doi: 10.1021/acschembio.7b00603.
  PUBMED    28938067
REFERENCE   2  (bases 1 to 9545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2017 Sep 29. doi: 10.1021/acschembio.7b00603."
COMMENT     Sequence Label: U6-hGRIN2B-CAG-ps-SaCas9
FEATURES             Location/Qualifiers
     source          1..9545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        271..346
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     enhancer        364..649
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        651..928
                     /label=chicken beta-actin promoter
     intron          929..1156
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1168..1177
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1177..1242
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1249..1269
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5851..5871
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5899..5952
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5953..6552
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5953..5972)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(6580..6597)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6586..6793
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6802..6942
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6802..6931
                     /label=AAV2 ITR
     rep_origin      7017..7472
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7104..7123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7314..7335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7489..7508)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7608..7630
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7668..7686)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7754..7858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7859..7927,7928..8719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8077..8096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8890..9478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9379..9398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gagtaggctg gtagatggag gttttagtac tctggaaaca gaatctacta
      301 aaacaaggca aaatgccgtg tttatctcgt caacttgttg gcgagatttt ttctagaggt
      361 acccgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      421 attgacgtca atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      481 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      541 tgacgtcaat gacggtaaat ggcccgcctg gcattgtgcc cagtacatga ccttatggga
      601 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
      661 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
      721 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc
      781 aggcggggcg ggggggggcg gggggggggg sggggcgagg cggagaggtg cggcggcagc
      841 caakcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
      901 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
      961 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     1021 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagctgagca agaggtaagg
     1081 gtttaaggga tggttggttg gtggggtatt aatgtttaat tacctggagc acctgcctga
     1141 aatcactttt tttcaggttg gaccggtgcc accatggact acaaagacca cgacggggat
     1201 tacaaagacc acgacattga ttacaaagac gatgatgata agatggctcc taagaagaag
     1261 cggaaggtcg gcattcacgg ggtgcctgcc gctaaaagaa attacatcct ggggctggat
     1321 attggaatca cctccgtggg atacggcatc attgactatg agacaaggga tgtcatcgac
     1381 gctggcgtgc gcctgttcaa ggaagcaaac gtggaaaaca atgagggccg gcggagcaag
     1441 cgaggagcaa ggcgcctgaa acgacggaga aggcacagaa tccagcgggt gaagaagctg
     1501 ctgttcgatt acaacctgct gaccgaccat tctgagctga gtggaatcaa tccatacgaa
     1561 gcaagagtga aaggcctgag tcagaagctg tcagaggaag agttttcagc agccctgctg
     1621 cacctggcaa agcgacgagg agtgcataac gtcaatgagg tggaagagga caccactagt
     1681 gtgatcaagc ccgacatgaa aatcaagctg aggatggagg gtgccgtgaa cggtcacccg
     1741 tttgcgatcg agggcgtggg actgggcaag ccgttcgagg gaaagcaaag catcgacctc
     1801 aaggtgaagg agggcggacc gctgcccttc gcctacgaca ttctgaccac tgccttctgc
     1861 tacggtaatc gcgtcttcgc aaagtatccc gaaaacatcg tggattactt taagcagagc
     1921 ttcccggagg gttactcctg ggagcgatcc atgaactacg aggacggagg gatctgcaac
     1981 gcgacgaacg atataacctt ggacggcgac tgctacattt acgaaattcg gttccgcggc
     2041 accaatttcc cagcgaatgg ccctgtaatg caaaaacgca cggtaaagtg ggagcccagc
     2101 accgagaacc tgtacgtccg cgacggagtc ctgaagggag acgtggtcat ggcgctttca
     2161 ttggaaggtg gcgggcacta cagatgcgat ttcaagacga cctataaggc gaaaaaggtg
     2221 gtccaactgc cggactacca tttcgtcgac caccacatcg agatcaagag ccacgacaaa
     2281 gactatagca acgtgaatct ccacgagcat gccgaggccc acagcgagct gcccaggcag
     2341 gccaagacta gtggcaacga gctgagcaca aaggaacaga tttcacgcaa tagcaaagcc
     2401 ctggaagaga agtatgtcgc tgagctgcag ctggaacggc tgaagaaaga tggcgaggtg
     2461 agagggtcaa tcaacaggtt caagaccagc gactacgtca aagaagcaaa gcagctgctg
     2521 aaagtgcaga aggcctacca ccagctggat cagtctttta ttgatacata tatcgacctg
     2581 ctggagactc ggagaaccta ctatgagggg ccaggagaag gcagtccctt cgggtggaaa
     2641 gacatcaagg aatggtacga gatgctgatg ggacattgca cctattttcc cgaagagctg
     2701 agaagcgtca agtacgccta taacgccgac ctgtataacg ccctgaacga cctgaacaac
     2761 ctggtcatca ctagggatga aaacgagaaa ctggaatact atgagaagtt ccagatcatt
     2821 gaaaacgtgt tcaagcagaa gaaaaagcct accctgaaac agattgccaa ggagatcctg
     2881 gtcaacgaag aggacatcaa gggctaccgg gtgacaagca ctggaaaacc agagttcact
     2941 aatctgaaag tgtatcacga tattaaggac atcaccgctc ggaaagaaat cattgagaac
     3001 gcagaactgc tggatcagat tgccaagatc ctgaccatct accagtcctc cgaggacatc
     3061 caggaagagc tgactaacct gaacagcgag ctgacccagg aagagatcga acagattagt
     3121 aatctgaagg gatacaccgg cacacacaac ctgagcctga aagccatcaa tctgattctg
     3181 gatgagctgt ggcatacaaa cgacaatcag attgctatct ttaaccggct gaagctggtc
     3241 ccaaaaaagg tggacctgtc ccagcagaag gagattccaa ccacactggt ggacgatttc
     3301 atcctgtctc ccgtggtcaa gagatccttt atccagagca tcaaagtgat caacgccatc
     3361 atcaagaagt acggcctgcc caatgatatc attatcgagc tggccaggga gaagaacagc
     3421 aaggacgctc agaagatgat taatgagatg cagaaacgaa accggcagac caatgaacgc
     3481 atcgaagaga ttatccgaac taccggaaaa gagaacgcta agtacctgat tgaaaaaatc
     3541 aagctgcacg atatgcagga gggcaagtgt ctgtattctc tggaggcaat ccctctagaa
     3601 gacctgctga acaatccatt caactacgag gtcgatcata ttatccccag aagcgtgtcc
     3661 ttcgacaata gcttcaacaa caaggtgctg gtcaagcagg aagagaacag caaaaagggc
     3721 aataggacac ctttccagta cctgtctagt tcagattcca agatttctta cgagactttt
     3781 aaaaagcaca tcctgaatct ggcaaaaggg ggatccgtga ttaaaccaga catgaagatc
     3841 aagctgcgta tggaaggcgc tgtaaatgga cacccgttcg cgattgaagg agttggcctt
     3901 gggaagcctt tcgagggaaa acagagtatt gaccttaaag tcaaagaagg cggacctctg
     3961 cctttcgcct atgacatctt gacaactgct ttctgttacg gcaacagggt attcgccaaa
     4021 tacccagaaa atatagtaga ctatttcaag cagtcgtttc ctgagggcta ctcttgggaa
     4081 cgaagcatga attacgaaga cgggggcatt tgtaacgcga caaacgacat aaccctggat
     4141 ggtgactgtt atatctatga aattcgattt agaggtacga actttcctgc caatggtcca
     4201 gttatgcaga agaggactgt gaaatgggag ccatccactg agaacttgta tgtgcgtgat
     4261 ggagtgctga agggtgatgt tgtcatggct ctgtcgcttg aaggaggtgg ccattaccga
     4321 tgtgacttca aaactactta taaagctaag aaggttgtcc agttgccaga ctatcacttt
     4381 gtggaccacc acattgagat taaaagccac gacaaagatt acagtaatgt taatctgcat
     4441 gagcacgccg aagcgcattc tgagctgccg aggcaggcca agggatccaa gggacgcatc
     4501 agtaagacca aaaaggagta cctgctggaa gagcgggaca ttaacagatt ctcagtccag
     4561 aaggacttca tcaaccggaa tctggtggac acaaggtacg ctactcgcgg gctgatgaat
     4621 ctgctgcgat cctatttccg ggtgaacaat ctggatgtga aagtcaaatc gattaacggc
     4681 gggttcacct cttttctgag gcgcaaatgg aagtttaaaa aggagaggaa caaaggctat
     4741 aagcaccatg cagaagatgc cctgattatc gctaatgcag acttcatctt taaggagtgg
     4801 aaaaagctgg acaaagccaa gaaagtgatg gagaaccaga tgttcgaaga gaagcaggca
     4861 gaatctatgc ccgaaatcga gacagaacag gagtacaagg agattttcat cactcctcac
     4921 cagatcaagc atatcaagga cttcaaggac tacaagtata gccaccgggt ggataaaaag
     4981 cctaacagag agctgattaa tgacaccctg tattccacac ggaaagacga taaggggaat
     5041 actctgatcg tgaacaatct gaacggactg tacgacaaag ataatgacaa gctgaaaaag
     5101 ctgattaaca aaagtcccga gaagctgctg atgtaccacc atgatcctca gacatatcag
     5161 aaactgaagc tgatcatgga gcagtacggc gacgagaaga acccactgta taagtactat
     5221 gaagagactg ggaactacct gaccaagtat agcaaaaagg ataatggccc cgtgatcaag
     5281 aagatcaagt actatgggaa caagctgaat gcccatctgg acatcaccga cgattaccct
     5341 aacagtagaa acaaggtggt caagctgtca ctgaagccat acaggttcga tgtctatctg
     5401 gacaacggcg tgtataaatt tgtgacagtc aagaatctgg atgtcatcaa aaaggagaac
     5461 tactatgaag tgaatagcaa gtgctacgaa gaggccaaaa agctgaaaaa gattagcaac
     5521 caggctgagt tcatcgcatc cttttacaac aacgacctga ttaagatcaa tggcgaactg
     5581 tatcgagtca ttggggtgaa caatgatctg ctgaaccgca tcgaagtgaa tatgattgac
     5641 atcacatacc gggagtatct ggaaaacatg aatgataagc gcccccctcg aattatcaaa
     5701 acaattgcct ctaagactca gagtatcaaa aagtactcca ctgacatcct gggcaacctg
     5761 tatgaagtca agtccaaaaa gcatcctcag attatcaaga agggaaaaag gccggcggcc
     5821 acgaaaaagg ccggccaagc tagcggctcc cccaagaaaa aacgcaaggt gggcttaatt
     5881 aacggaagcg gcagtggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag
     5941 aatcctggcc caatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6001 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6061 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6121 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6181 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6241 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6301 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6361 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6421 cccgccttcc tggagacctc cgcgccccac aacctcccct tctacgagcg gctcggcttc
     6481 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6541 cccggtgcct gagaattcta actagagctc gctgatcagc ctcgactgtg ccttctagtt
     6601 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6661 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6721 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gagaatagca
     6781 ggcatgctgg ggagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     6841 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     6901 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     6961 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     7021 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7081 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7141 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7201 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     7261 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7321 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
     7381 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7441 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     7501 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     7561 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     7621 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     7681 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     7741 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7801 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7861 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7921 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7981 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8041 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8101 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8161 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8221 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8281 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     8341 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     8401 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     8461 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     8521 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggaagccg
     8581 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     8641 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8701 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8761 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8821 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8881 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8941 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9001 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     9061 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9121 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     9181 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     9241 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     9301 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     9361 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     9421 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     9481 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     9541 tcgaa
//
