LOCUS       sequence-199004-       10826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct of human codon-adapted anion 
            channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via 
            a NotI site in the pFUGW vector backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10826)
  AUTHORS   Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL
  TITLE     Natural light-gated anion channels: A family of microbial rhodopsins
            for advanced optogenetics.
  JOURNAL   Science. 2015 Jun 25. pii: aaa7484.
  PUBMED    26113638
REFERENCE   2  (bases 1 to 10826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Jun 25. pii: aaa7484."
COMMENT     Sequence Label: pFUGW-hGtACR2-EYFP
FEATURES             Location/Qualifiers
     source          1..10826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..47
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(166..186)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        171..500
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      351..486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     413..432
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        548..595
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             614..988
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1118..1239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1155..1174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1209..1228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1288..1304)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1288..1304)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1301..1323)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1312..1328
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1336..1342,1343..1360,1361..1366))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1381..1402
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1519..1536)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1690..2278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1770..1789)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2449..3240,3241..3309))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3072..3091
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3310..3414)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3489..3508)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3680..4059
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4061..4259
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4214..4234
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4277..4457
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4504..4629
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5122..5355
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5540..5584
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5882..5999
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6091..7302
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7139..7158
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(8219..8221,8222..8935)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8261..8282)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8522..8541)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8869..8890
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8960..9548
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9013..9033)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9431..9442)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9551..9567)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9552..9568)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10073..10253
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10279..10296)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10285..10509
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10555..10826,1..157)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10642..10661)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
       61 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
      121 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
      181 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
      241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
      301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
      361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
      421 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
      481 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
      541 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
      601 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
      661 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
      721 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
      781 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
      841 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
      901 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
      961 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     1021 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     1081 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     1141 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1201 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     1261 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     1321 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1381 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1441 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1501 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1561 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1621 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1681 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1741 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1801 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1861 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1921 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1981 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2041 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2101 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2161 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2221 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2281 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2341 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2401 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2461 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2521 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2581 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2641 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2701 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2761 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2821 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2881 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2941 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3001 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3061 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3121 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3181 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3241 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3301 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3361 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3421 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     3481 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     3541 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     3601 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     3661 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     3721 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     3781 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     3841 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     3901 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     3961 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     4021 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     4081 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     4141 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     4201 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
     4261 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     4321 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     4381 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     4441 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     4501 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     4561 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     4621 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     4681 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     4741 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     4801 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     4861 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     4921 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     4981 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     5041 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     5101 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     5161 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     5221 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     5281 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     5341 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     5401 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     5461 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     5521 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     5581 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     5641 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     5701 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     5761 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     5821 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
     5881 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     5941 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     6001 gggtttatta cagggacagc agagatccag tttggttaat cgcatgctag ctagttcgaa
     6061 ccttaattaa cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg gcgcctcccg
     6121 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc
     6181 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
     6241 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
     6301 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
     6361 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
     6421 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
     6481 gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
     6541 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
     6601 gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg
     6661 aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg
     6721 ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg
     6781 agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact
     6841 cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt
     6901 acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat
     6961 gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag
     7021 ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag
     7081 ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg
     7141 aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag
     7201 gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa
     7261 ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg ggctgcaggt
     7321 cgactctaga ggatccatgg cctcccaggt cgtgtacggc gagtgggcca gcacccacac
     7381 agagtgctac aacatgagca ggatcgacag caccttcgtg agcctgctgc agctggtgtg
     7441 ggccgtggtg agcggatgcc agaccatctt catgatcagc agggccccca aggtgccctg
     7501 ggagagcgtg tacctgccct tcgtggagtc catcacatac gccctggcca gcaccggcaa
     7561 tggcacactg cagatgaggg acggcaggtt ctttccctgg tccaggatgg ccagctggct
     7621 gtgcacatgc cccattatgc tgggccagat ctccaacatg gctctggtga agtacaagag
     7681 catccctctg aaccccatcg cccaggccgc cagcattatc agggtggtga tgggcattac
     7741 cgctaccatc tcccccgccg agtacatgaa gtggctgttc ttcttcttcg gcgctacctg
     7801 cctggtcttc gaatacagcg tggtgttcac catcttccag gtgggactgt acggcttcga
     7861 gtccgtgggc acccctctgg cccagaaggt ggtggtgagg atcaagatgc tgaggctgat
     7921 cttcttcatc gcctggacca tgttccccat cgtgtggctg atttccccca ccggcgtgtg
     7981 cgtgatccac gagaacgtga gcgccatcct gtatctgctc gccgacggcc tgtgcaagaa
     8041 cacctacggc gtgatcctgt ggtccaccgc ctggggagtg ctcgagggaa agtgggaccc
     8101 ggcttgcctc cctggccagg agaagcctga ggccgacgac cccttcggac tgaaccacga
     8161 gaagaatgcc ccccccaacg acgaggtcaa catcagaatg ttcggcaggg cggccgccgt
     8221 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     8281 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     8341 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     8401 gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     8461 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     8521 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     8581 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     8641 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     8701 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     8761 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     8821 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     8881 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagaatt
     8941 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     9001 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     9061 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     9121 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     9181 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     9241 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     9301 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     9361 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     9421 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     9481 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     9541 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     9601 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     9661 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     9721 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     9781 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     9841 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     9901 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     9961 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
    10021 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
    10081 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    10141 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    10201 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
    10261 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
    10321 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
    10381 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
    10441 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
    10501 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
    10561 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
    10621 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
    10681 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
    10741 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
    10801 cctgatagac ggtttttcgc cctttg
//
