LOCUS       sequence-199006-       13743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13743)
  AUTHORS   Zhou XX, Zou X, Chung HK, Gao Y, Liu Y, Qi LS, Lin MZ
  TITLE     A Single-Chain Photoswitchable CRISPR-Cas9 Architecture for 
            Light-Inducible Gene Editing and Transcription.
  JOURNAL   ACS Chem Biol. 2017 Sep 29. doi: 10.1021/acschembio.7b00603.
  PUBMED    28938067
REFERENCE   2  (bases 1 to 13743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2017 Sep 29. doi: 10.1021/acschembio.7b00603."
COMMENT     Sequence Label: VPR-ps-dSpCas9
FEATURES             Location/Qualifiers
     source          1..13743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     intron          277..1293
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1217..1239
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1301..1320
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1360..1959
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1360..1379)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1856..1876
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1997..2585
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2050..2070)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2468..2479)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2606..3105
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2641..2664)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3038..3057
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3129..3138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3138..3287
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             3294..3314
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3744..4115
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             4134..4703
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             10215..10235
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     repeat_region   10425..10487
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10538..10558)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10538..10556)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10577..10593)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10577..10593)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10590..10612)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10601..10617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10625..10631,10632..10649,10650..10655))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10670..10691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10808..10825)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10979..11567)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11059..11078)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11738..12529,12530..12598))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12361..12380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12599..12703)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(12729..13184)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12866..12887)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13078..13097
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13311..13333
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13325..13342
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13326..13342
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13352..13371
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13352..13370
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   13400..13434
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 cgcgcgccag gcggggcggg gcggggcggg gggcggggcg csgcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
      481 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
      541 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
      601 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
      661 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
      721 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
      781 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
      841 ggggggtggc ggcaggtggg ggtgccgggm sgggcggggc cgcctcgggc cggggagggc
      901 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
      961 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1021 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
     1081 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     1141 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
     1201 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     1261 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     1321 ctgtctcatc attttggcaa agaattcgcc accatgacca tgaccgagta caagcccacg
     1381 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     1441 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     1501 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     1561 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     1621 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     1681 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     1741 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     1801 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     1861 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     1921 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag tttaaaccat gcaagcttga
     1981 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     2041 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     2101 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2161 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     2221 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     2281 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     2341 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     2401 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     2461 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     2521 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     2581 cccgcatcct gcaggtaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     2641 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     2701 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     2761 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     2821 aggacgtgac aaatggaagt agcacgtctc actaggctcg tgcagatgga cagcaccgct
     2881 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     2941 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     3001 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     3061 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc
     3121 ttgtcgacgc caccatggat gctttagacg attttgactt agatatgctt ggttcagacg
     3181 cgttagacga cttcgaccta gacatgttag gctcagatgc attggacgac ttcgatttag
     3241 atatgttggg ctccgatgcc ctagatgact ttgatctaga tatgctaggt agtcccaaaa
     3301 agaagaggaa agtgcccggg cagtatctgc ccgacacaga tgatagacac cgaatcgaag
     3361 agaaacgcaa gcgaacgtat gaaaccttca aatcgatcat gaagaaatcg cccttctcgg
     3421 gtccgaccga tcccaggccc ccaccgagaa ggattgcggt cccgtcccgc tcgtcggcca
     3481 gcgtgccgaa gcctgcgccg cagccctacc ccttcacgtc gagcctgagc acaatcaatt
     3541 atgacgagtt cccgacgatg gtgttcccct cgggacaaat ctcacaagcc tcggcgctcg
     3601 caccagcgcc tccccaagtc cttccgcaag cgcctgcccc agcgcctgca ccggcaatgg
     3661 tgtccgccct cgcacaggcc cctgcgcccg tccccgtgct cgcgcctgga ccgccccagg
     3721 cggtcgctcc accggctccg aagccgacgc aggccggaga gggaacactc tccgaagcac
     3781 ttcttcaact ccagtttgat gacgaggatc ttggagcact ccttggaaac tcgacagacc
     3841 ctgcggtgtt taccgacctc gcgtcagtag ataactccga atttcagcag cttttgaacc
     3901 agggtatccc ggtcgcgcca catacaacgg agcccatgtt gatggaatac cccgaagcaa
     3961 tcacgagact tgtgacggga gcgcagcggc ctcccgatcc cgcacccgca cctttggggg
     4021 cacctggcct ccctaacgga cttttgagcg gcgacgagga tttctcctcc atcgccgata
     4081 tggatttctc agccttgctg tcacagattt ccagcggctc tggcagcggc agccgggatt
     4141 ccagggaagg gatgtttttg ccgaagcctg aggccggctc cgctattagt gacgtgtttg
     4201 agggccgcga ggtgtgccag ccaaaacgaa tccggccatt tcatcctcca ggaagtccat
     4261 gggccaaccg cccactcccc gccagcctcg caccaacacc aaccggtcca gtacatgagc
     4321 cagtcgggtc actgaccccg gcaccagtcc ctcagccact ggacccagcg cccgcagtga
     4381 ctcccgaggc cagtcacctg ttggaggacc ccgatgaaga gacgagccag gctgtcaaag
     4441 cccttcggga gatggccgat actgtgattc cccagaagga agaggctgca atctgtggcc
     4501 aaatggacct ttcccatccg cccccaaggg gccatctgga tgagctgaca accacacttg
     4561 agtccatgac cgaggatctg aacctggact cacccctgac cccggaattg aacgagattc
     4621 tggatacctt cctgaacgac gagtgcctct tgcatgccat gcatatcagc acaggactgt
     4681 ccatcttcga cacatctctg tttcatatgt caagcatgga caagaagtac agcatcggcc
     4741 tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca
     4801 gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg
     4861 gcgccctgct gttcgacagc ggagaaacag ccgaggccac ccggctgaag agaaccgcca
     4921 gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg
     4981 agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag
     5041 aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc
     5101 acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg
     5161 ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc
     5221 tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg
     5281 tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca
     5341 aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc
     5401 tgcccggcga gaagaagaat ggcctgttcg gcaacctgat tgccctgagc ctgggcctga
     5461 cccccaactt caagagcaac ttcgacctgg ccactagtgt gatcaagccc gacatgaaaa
     5521 tcaagctgag gatggagggt gccgtgaacg gtcacccgtt tgcgatcgag ggcgtgggac
     5581 tgggcaagcc gttcgaggga aagcaaagca tcgacctcaa ggtgaaggag ggcggaccgc
     5641 tgcccttcgc ctacgacatt ctgaccactg ccttctgcta cggtaatcgc gtcttcgcaa
     5701 agtatcccga aaacatcgtg gattacttta agcagagctt cccggagggt tactcctggg
     5761 agcgatccat gaactacgag gacggaggga tctgcaacgc gacgaacgat ataaccttgg
     5821 acggcgactg ctacatttac gaaattcggt tccgcggcac caatttccca gcgaatggcc
     5881 ctgtaatgca aaaacgcacg gtaaagtggg agcccagcac cgagaacctg tacgtccgcg
     5941 acggagtcct gaagggagac gtgatcatgg cgctttcatt ggaaggtggc gggcactaca
     6001 gatgcgattt caagacgacc tataaggcga aaaaggtggt ccaactgccg gactaccatt
     6061 tcgtcgacca ccacatcgag atcaagagcc acgacaaaga ctatagcaac gtgaatctcc
     6121 acgagcatgc cgaggcccac agcgagctgc ccaggcaggc caagactagt gaggatgcca
     6181 aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
     6241 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga
     6301 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca
     6361 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc
     6421 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca
     6481 tcgatggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga
     6541 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc
     6601 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc
     6661 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga
     6721 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg
     6781 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg
     6841 acaagggcgc cagcgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc
     6901 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtacaacg
     6961 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg
     7021 agcagaaaaa agccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc
     7081 agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg
     7141 tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg
     7201 acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc
     7261 tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt
     7321 tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga
     7381 gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc
     7441 tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga
     7501 cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc
     7561 acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg
     7621 tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa
     7681 tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc
     7741 ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa
     7801 acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt
     7861 acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac gctatcgtgc
     7921 ctcagagctt tctgaaggac gactccatcg ataacaaagt gctgactcgg agcgacaaga
     7981 accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact
     8041 ggcgccagct gctgaatgcc aagctgatta cccagaggaa gttcgacaat ctgaccaagg
     8101 ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg
     8161 aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt
     8221 acgacgagaa cgacaaactg atccgggaag tgaaagtgat caccctgaag tccaagctgg
     8281 tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc
     8341 acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta
     8401 agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg
     8461 ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca
     8521 tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga
     8581 tcgagacaaa cggcgaaaca ggcgagatcg tgtgggataa gggccgggac tttgccaccg
     8641 tgcggaaagt gctgtctatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag
     8701 gcggcttcag caaagagtct atcctgccca agaggaacag cgacaagctg atcgccagaa
     8761 agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg
     8821 tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc
     8881 tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg
     8941 aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc
     9001 tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg
     9061 gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg
     9121 agaagctgaa gggatccgtg attaaaccag acatgaagat caagctgcgt atggaaggcg
     9181 ctgtaaatgg acacccgttc gcgattgaag gagttggcct tgggaagcct ttcgagggaa
     9241 aacagagtat tgaccttaaa gtcaaagaag gcggacctct gcctttcgcc tatgacatct
     9301 tgacaactgc tttctgttac ggcaacaggg tattcgccaa atacccagaa aatatagtag
     9361 actatttcaa gcagtcgttt cctgagggct actcttggga acgaagcatg aattacgaag
     9421 acgggggcat ttgtaacgcg acaaacgaca taaccctgga tggtgactgt tatatctatg
     9481 aaattcgatt tagaggtacg aactttcctg ccaatggtcc agttatgcag aagaggactg
     9541 tgaaatggga gccatccact gagaacttgt atgtgcgtga tggagtgctg aagggtgatg
     9601 ttatcatggc tctgtcgctt gaaggaggtg gccattaccg atgtgacttc aaaactactt
     9661 ataaagctaa gaaggttgtc cagttgccag actatcactt tgtggaccac cacattgaga
     9721 ttaaaagcca cgacaaagat tacagtaatg ttaatctgca tgagcacgcc gaagcgcatt
     9781 ctgagctgcc gaggcaggcc aagggatccc ccgaggataa tgagcagaaa cagctgtttg
     9841 tggaacagca caaacactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga
     9901 gagtgatcct ggccgacgct aatctggaca aggtgctgag cgcctacaac aagcacagag
     9961 acaagcctat cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg
    10021 gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca
    10081 ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac
    10141 ggatcgacct gtctcagctg ggaggcgaca aaaggccggc ggccacgaaa aaggccggcc
    10201 aagctagcgg ctcccccaag aaaaaacgca aggtgtaact cgagctagtt aaaagttttg
    10261 ttactttata gaagaaattt tgagtttttg ttttttttta ataaataaat aaacataaat
    10321 aaattgtttg ttgaatttat tattagtatg taagtgtaaa tataataaaa cttaatatct
    10381 attcaaatta ataaataaac ctcgatatac agaccgataa aacacatgcg tcaattttac
    10441 gcatgattat ctttaacgta cgtcacaata tgattatctt tctagggtta atctagtata
    10501 cgcgtatgcg gccgcttaat taatccagct tttgttccct ttagtgaggg ttaattgcgc
    10561 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    10621 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    10681 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    10741 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    10801 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    10861 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    10921 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    10981 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    11041 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    11101 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    11161 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    11221 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    11281 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    11341 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    11401 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    11461 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    11521 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    11581 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    11641 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    11701 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    11761 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    11821 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    11881 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    11941 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    12001 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    12061 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    12121 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    12181 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    12241 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    12301 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    12361 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    12421 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    12481 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    12541 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    12601 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    12661 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    12721 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
    12781 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
    12841 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
    12901 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
    12961 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
    13021 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
    13081 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
    13141 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
    13201 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    13261 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    13321 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
    13381 gcgcgccatt ctagattaac cctagaaaga tagtctgcgt aaaattgacg catgcattct
    13441 tgaaatattg ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct
    13501 cagtcgccgc ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt
    13561 tgaactataa cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa
    13621 gatttaactc atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt
    13681 atatatatat tttcttgtta tagatatcaa ctagaatgct agcatgggcc cagatctcca
    13741 tgg
//
