LOCUS       sequence-199016-        2758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conductive Curli Protein csgAWY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Kalyoncu E, Ahan RE, Olmez TT, Seker UOS
  TITLE     Genetically encoded conductive protein nanofibers secreted by 
            engineered cells
  JOURNAL   RSC Adv. 2017, Issue 52. doi: 10.1039/C7RA06289C
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RSC Adv. 
            2017, Issue 52. doi: 10.1039/C7RA06289C"
COMMENT     Sequence Label: pZa-tetO-csgAWY-CmR
FEATURES             Location/Qualifiers
     source          1..2758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..32
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(99..201)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    240..258
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    265..283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(536..554,555..560,561..577,578..583,584..609)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    536..554
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    561..579
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             644..655
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1168..1254
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1289..1306)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1423..1968)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1503..1522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2082..2176)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2197..2758,1..98))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
       61 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
      121 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
      181 gaacctctta cgtgccgatc aacgtctcat tttcgccaga tatcgacgtc aaagggccct
      241 ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag cacagtcgac
      301 acccaaatcc aggaggtgat tggtagtggt ggttaatgaa aattaactta ctactaccat
      361 atatctctag atgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
      421 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
      481 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttc tcgagtccct
      541 atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca tcagcaggac
      601 gcactgaccg aattctacca ttcacctctt ggatttgggt attaaagagg agaaaggtac
      661 catgaaactt ttaaaagtag cagcaattgc agcaatcgta ttctccggta gcgctctggc
      721 aggtgttgtt cctcagtacg gcggcggcgg taaccacggt ggtggcggta ataatagcgg
      781 cccaaattct gagctgaaca tttaccagta cggtggcggt aactctgcac ttgctctgca
      841 aactgatgcc cgtaactctg acttgactat tacccagcat ggcggcggta atggtgcaga
      901 tgttggtcag ggctcagatg acagctcaat cgatctgacc caacgtggct tcggtaacag
      961 cgctactctt gatcagtgga acggcaaaaa ttctgaaatg acggttaaac agttcggtgg
     1021 tggcaacggt gctgcagttg accagactgc atctaactcc tccgtcaacg tgactcaggt
     1081 tggctttggt aacaacgcga ccgctcatca gtactactat cgcaaataca aagagtggga
     1141 tgattattaa acgcgtgcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1201 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     1261 ccctagacct agggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
     1321 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
     1381 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
     1441 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     1501 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
     1561 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
     1621 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     1681 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
     1741 aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc
     1801 gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta
     1861 cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt
     1921 ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt
     1981 attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa tgtagcacct
     2041 gaagtcagcc ccatacgata taagttgtta ctagtgcttg gattctcacc aataaaaaac
     2101 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     2161 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     2221 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     2281 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     2341 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     2401 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     2461 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     2521 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     2581 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa
     2641 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     2701 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggtta
//
