LOCUS       sequence-199054-        5429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  medium copy plasmid (pBR322 backbone) expressing the unstable GFP 
            variant GFP-OVA under control of the hydrogen peroxide-inducible 
            promoter katGp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Burton NA, Schurmann N, Casse O, Steeb AK, Claudi B, Zankl J, 
            Schmidt A, Bumann D
  TITLE     Disparate impact of oxidative host defenses determines the fate of 
            Salmonella during systemic infection in mice.
  JOURNAL   Cell Host Microbe. 2014 Jan 15;15(1):72-83. doi: 
            10.1016/j.chom.2013.12.006.
  PUBMED    24439899
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2013.12"; journalName: "Cell Host Microbe"; date: 
            "2014-01-15- 15"; volume: "15"; issue: "1"; pages: "72-83"
COMMENT     Sequence Label: pNB23
FEATURES             Location/Qualifiers
     source          1..5429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     83..103
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(268..285)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      438..524
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      616..643
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        662..753
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(754..822,823..1614)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(972..991)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1785..2373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2274..2293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2527..2544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2559..2699
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2625..2644)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2785..2807
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2801..2992)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     4450..4469
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             join(4859..5429,1..143)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(4859..5429,1..143)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4889..4917)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
       61 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
      121 atggcatgga tgaactatac aaagaatctc tgaaaatctc tcaggctgtt cacgctgctc
      181 acgctgaaat caacgaagct ggtcgtgaag tagtaggtta actgcagcca agcttggctg
      241 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
      301 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
      361 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt
      421 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      481 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
      541 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
      601 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
      661 ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      721 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      781 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      841 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      901 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      961 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     1021 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1081 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1141 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1201 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1261 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1321 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1381 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1441 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1501 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1561 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1621 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1681 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1741 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1801 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1861 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1921 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1981 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2041 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2101 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2161 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2221 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2281 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2341 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2401 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2461 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2521 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     2581 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     2641 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     2701 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     2761 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     2821 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac
     2881 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     2941 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
     3001 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
     3061 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
     3121 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
     3181 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
     3241 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
     3301 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
     3361 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
     3421 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg
     3481 agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt
     3541 tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga
     3601 atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc
     3661 gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc
     3721 gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga
     3781 agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac
     3841 ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat
     3901 cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc
     3961 gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc
     4021 agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat
     4081 catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac
     4141 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
     4201 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc
     4261 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     4321 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     4381 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     4441 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     4501 gccggccacg atgcgtccgg cgtagagatc cccacttcta cacgttgaat cgtgcggaaa
     4561 tgagttatgc catttgccac acgctgggcg taagaccggg tttataacct ccagctccct
     4621 gccgggagct ttattacaac tcatattgat ctacatctct gtaactaaaa atataaaagg
     4681 tattagctat cgaatctgtg gattaattca actatatcta tttgctcctg gtgtatatcg
     4741 taacggtaac actttaaaag ggagctgaga tatgagcacg accgacgata cccataacac
     4801 gttatccact ggaaaatgtc ctttccatca ggtctagatt taagaaggag atatacatat
     4861 gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     4921 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     4981 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     5041 cactactttc gcgtatggtc ttcaatgctt tgcgagatac ccagatcata tgaaacagca
     5101 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     5161 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
     5221 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
     5281 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
     5341 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
     5401 ttatcaacaa aatactccaa ttggcgatg
//
