LOCUS       sequence-199055-        5562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  medium copy plasmid for expression of a variant of the fluorescent 
            timer protein suitable for bacteria under the control of the 
            constitutive ybaJp promoter (works in E. coli and Salmonella 
            enterica).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Claudi B, Sprote P, Chirkova A, Personnic N, Zankl J, Schurmann N, 
            Schmidt A, Bumann D
  TITLE     Phenotypic variation of Salmonella in host tissues delays 
            eradication by antimicrobial chemotherapy.
  JOURNAL   Cell. 2014 Aug 14;158(4):722-733. doi: 10.1016/j.cell.2014.06.045.
  PUBMED    25126781
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.06"; journalName: "Cell"; date: "2014-08-14- 
            14"; volume: "158"; issue: "4"; pages: "722-733"
COMMENT     Sequence Label: pBR322-TIMER
FEATURES             Location/Qualifiers
     source          1..5562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(159..181)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    267..407
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     322..341
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(422..439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(593..1181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(673..692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1352..2143,2144..2212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1975..1994
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2213..2304)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(2323..2350)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2442..2528)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2681..2698
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(2799..2822)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2997..3016)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3214..3234
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3266..3284
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(4059..4078)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             join(5536..5562,1..165)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg
       61 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac
      121 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
      181 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
      241 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
      301 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
      361 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
      421 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      481 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      541 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      601 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      661 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      721 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      781 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      841 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      901 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      961 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1021 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1081 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1141 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1201 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1261 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1321 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1381 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1441 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1501 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1561 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1621 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1681 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1741 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1801 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1861 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1921 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     1981 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2041 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2101 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2161 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2221 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2281 aatgtattta gaaaaataaa caaaagagtt tgtagaaacg caaaaaggcc atccgtcagg
     2341 atggccttct gcttaatttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
     2401 ccgggccgtt gcttcgcaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
     2461 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
     2521 tttatttgat gcctggcagt tccctactct cgcatgggga gaccccacac taccatcggc
     2581 gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg cgctactgcc
     2641 gccaggcaaa ttctgtttta tcagaccgct tctgcgttct gatttaatct gtatcaggct
     2701 gaaaatcttc tctcatccgc caaaacagcc aagcttctac aggaacaggt ggtagcggcc
     2761 ctcggtgcgt tcgtactgct ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag
     2821 cttggtgtcc acgtagtagt agccgggcag ttgcacgggc ttcttggcca tgtagatgga
     2881 cttgaactcc accaggtagt ggccgccgtc cttcagcttc agggccttgt ggatctcgcc
     2941 cttcagcacg ccgtcgcggg ggtacaggcg ctcggtggag ggctcccagc ccatagtctt
     3001 cttctgcatt acggggccgt cggaggggaa gttcacgccg atgaacttca ccttgtagat
     3061 gaagcagccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa
     3121 gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcttgt agtcggggat
     3181 gtcggcgggg tgcttcacgt acaccttgga gccgtactgg aactgggggg acaggatgtc
     3241 ccaggcgaag ggcagggggc cgcccttggt caccttcagc ttggcggtct gggtgccctc
     3301 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
     3361 catgcgcact ttgaagcgca tgaactcctt gatgacgtcc tcggtggatg ccatatgtat
     3421 atctccttct taaatctaga ggatctctat cagctcattg agctgcaggt tgactgctga
     3481 ggtcgggtca ttaacccagc catggttgct ttcttcaagg tttgcaagac agtcatgata
     3541 cagggtttcg caaagaaatt taagctgtgc tatatcatgt cttttgggtg agtattcgtc
     3601 cataatgcat ccccttttta gtggcttaaa ctaaccgaac accttcggga tggatacaac
     3661 taactaacct ggtacttccc acgtcacctt atgatttaac tataatctac tctaaaaaac
     3721 ttttcatcgt catattacta aaaattacaa ttgcgtgaaa gccgcgtgtt gccatacttt
     3781 ttcgaatttt aacagtgatc gtcggtcgcc taaaaaggaa ccagttttat tgtttgtgta
     3841 atcattgggt taacgttttt tagcttttca ggctaaaaca atagactctg acaggagaaa
     3901 atagccagga atattcttaa tatttcttaa ttaatggctg aattaagaaa tggccaactt
     3961 tcctaagaaa agcctttaac gcagtaagga ttatacgatc ctctacgccg gacgcatcgt
     4021 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     4081 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     4141 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     4201 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     4261 taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     4321 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     4381 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
     4441 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     4501 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
     4561 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     4621 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     4681 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
     4741 cctaacttcg atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
     4801 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt
     4861 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct
     4921 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
     4981 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg
     5041 cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg
     5101 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc
     5161 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg
     5221 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
     5281 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
     5341 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
     5401 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
     5461 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct
     5521 tacacggagg catcagtgac caaacaggaa aaaaccgccc tt
//
