LOCUS       sequence-199056-        4946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  low copy plasmid for expression of a variant of the fluorescent 
            timer protein suitable for bacteria under the control of the 
            constitutive ybaJp promoter (works in E. coli and Salmonella 
            enterica).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Claudi B, Sprote P, Chirkova A, Personnic N, Zankl J, Schurmann N, 
            Schmidt A, Bumann D
  TITLE     Phenotypic variation of Salmonella in host tissues delays 
            eradication by antimicrobial chemotherapy.
  JOURNAL   Cell. 2014 Aug 14;158(4):722-733. doi: 10.1016/j.cell.2014.06.045.
  PUBMED    25126781
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.06"; journalName: "Cell"; date: "2014-08-14- 
            14"; volume: "158"; issue: "4"; pages: "722-733"
COMMENT     Sequence Label: pSC101_TIMER
FEATURES             Location/Qualifiers
     source          1..4946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      64..158
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      650..872
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             920..1870
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(2408..2494)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2622..2641
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3416..3434)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(3466..3486)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3684..3703
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3878..3901
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(3988..4006)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(4185..4946,1..33)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4239..4258)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4849..4868
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gagagctcgc
       61 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
      121 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagcactagg gacagtaaga
      181 cgggtaagcc tgttgatgat accgctgcct tactgggtgc attagccagt ctgaatgacc
      241 tgtcacggga taatccgaag tggtcagact ggaaaatcag agggcaggaa ctgctgaaca
      301 gcaaaaagtc agatagcacc acatagcaga cccgccataa aacgccctga gaagcccgtg
      361 acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag gcgcctgtag
      421 tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat gtcacgaaaa
      481 agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg atttgcccga
      541 gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta gaggagcaaa
      601 cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg agttatacac
      661 agggctggga tctattcttt ttatcttttt ttattctttc tttattctat aaattataac
      721 cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag agggtctagc
      781 agaatttaca agttttccag caaaggtcta gcagaattta cagataccca caactcaaag
      841 gaaaaggact agtaattatc attgactagc ccatctcaat tggtatagtg attaaaatca
      901 cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg aactagcgat
      961 tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg tggcactact
     1021 caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca cttataacca
     1081 atacgctcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag ctaaagcaac
     1141 cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag gctttgagat
     1201 tttccagtgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag tttttagtga
     1261 agagatattg ccttatcttt tccagttaaa aaaattcata aaatataatc tggaacatgt
     1321 taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa aagaactaac
     1381 acaaaagaaa actcacaagg caaatataga gattagcctt gatgaattta agttcatgtt
     1441 aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt tgaaaccaat
     1501 aagtaaagat ttaaacactt acagcaatat gaaattggtg gttgataagc gaggccgccc
     1561 gactgatacg ttgattttcc aagttgaact agatagacaa atggatctcg taaccgaact
     1621 tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta catcagattc
     1681 ctacctacat aacggactaa gaaaaacact acacgatgct ttaactgcaa aaattcagct
     1741 caccagtttt gaggcaaaat ttttgagtga catgcaaagt aagtatgatc tcaatggttc
     1801 gttctcatgg ctcacgcaaa aacaacgaac cacactagag aacatactgg ctaaatacgg
     1861 aaggatctga ggttcttatg gctcttgtat ctatcagtga agcatcaaga ctaacaaaca
     1921 aaagtagaac aactgttcac cgttacatat caaagggaaa actgtccata tgcacagatg
     1981 aaaacggtgt aaaaaagata gatacatcag agcttttacg agtttttggt gcattcaaag
     2041 ctgttcacca tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca cctaatagaa
     2101 caggtgaaac cagtaaaaca aagcaactag aacatgaaat tgaacacctg agacaacttg
     2161 ttacagctca acagtcacac atagacagcc tgaaacaggc gatgctgctt atcgaatcaa
     2221 agctgccgac aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca tggcaattct
     2281 ggaagaaata gcgctttcag ccggcaaacc ggctgaagcc ggatctgcga ttctgataac
     2341 aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgtaccc taggtctagg
     2401 gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     2461 cccagtcttt cgactgagcc tttcgtttta tttgatgcct ctagagcttg catgcccaag
     2521 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
     2581 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
     2641 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
     2701 atcgtataat ccttactgcg ttaaaggctt ttcttaggaa agttggccat ttcttaattc
     2761 agccattaat taagaaatat taagaatatt cctggctatt ttctcctgtc agagtctatt
     2821 gttttagcct gaaaagctaa aaaacgttaa cccaatgatt acacaaacaa taaaactggt
     2881 tcctttttag gcgaccgacg atcactgtta aaattcgaaa aagtatggca acacgcggct
     2941 ttcacgcaat tgtaattttt agtaatatga cgatgaaaag ttttttagag tagattatag
     3001 ttaaatcata aggtgacgtg ggaagtacca ggttagttag ttgtatccat cccgaaggtg
     3061 ttcggttagt ttaagccact aaaaagggga tgcattatgg acgaatactc acccaaaaga
     3121 catgatatag cacagcttaa atttctttgc gaaaccctgt atcatgactg tcttgcaaac
     3181 cttgaagaaa gcaaccatgg ctgggttaat gacccgacct cagcagtcaa cctgcagctc
     3241 aatgagctga tagagatcct ctagatttaa gaaggagata tacatatggc atccaccgag
     3301 gacgtcatca aggagttcat gcgcttcaaa gtgcgcatgg agggctccgt gaacggccac
     3361 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     3421 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     3481 cagtacggct ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg
     3541 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     3601 accgtgaccc aggactcctc cctgcaggac ggctgcttca tctacaaggt gaagttcatc
     3661 ggcgtgaact tcccctccga cggccccgta atgcagaaga agactatggg ctgggagccc
     3721 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca caaggccctg
     3781 aagctgaagg acggcggcca ctacctggtg gagttcaagt ccatctacat ggccaagaag
     3841 cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac
     3901 gaggactaca ccatcgtgga gcagtacgaa cgcaccgagg gccgctacca cctgttcctg
     3961 tagaagcttc gaggtgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4021 tcatgataat aatggtttct tagacgtcgg aattgccagc tggggcgccc tctggtaagg
     4081 ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca
     4141 ggggatcaag atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     4201 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     4261 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     4321 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     4381 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     4441 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     4501 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     4561 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     4621 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     4681 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     4741 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     4801 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     4861 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     4921 tcgccgctcc cgattcgcag cgcatc
//
