LOCUS       sequence-199064-        2758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conductive Curli Protein csgAYW.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Kalyoncu E, Ahan RE, Olmez TT, Seker UOS
  TITLE     Genetically encoded conductive protein nanofibers secreted by 
            engineered cells
  JOURNAL   RSC Adv. 2017, Issue 52. doi: 10.1039/C7RA06289C
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RSC Adv. 
            2017, Issue 52. doi: 10.1039/C7RA06289C"
COMMENT     Sequence Label: pZa-tetO-csgAYW-CmR
FEATURES             Location/Qualifiers
     source          1..2758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(39..141)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    205..223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(476..494,495..500,501..517,518..523,524..549)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    476..494
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    501..519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             584..595
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1108..1194
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1229..1246)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1363..1908)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1443..1462)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2022..2116)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2137..2758,1..38))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2711..2730
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
       61 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
      121 gaacctctta cgtgccgatc aacgtctcat tttcgccaga tatcgacgtc aaagggccct
      181 ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag cacagtcgac
      241 acccaaatcc aggaggtgat tggtagtggt ggttaatgaa aattaactta ctactaccat
      301 atatctctag atgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
      361 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
      421 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttc tcgagtccct
      481 atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca tcagcaggac
      541 gcactgaccg aattctacca ttcacctctt ggatttgggt attaaagagg agaaaggtac
      601 catgaaactt ttaaaagtag cagcaattgc agcaatcgta ttctccggta gcgctctggc
      661 aggtgttgtt cctcagtacg gcggcggcgg taaccacggt ggtggcggta ataatagcgg
      721 cccaaattct gagctgaaca tttaccagta cggtggcggt aactctgcac ttgctctgca
      781 aactgatgcc cgtaactctg acttgactat tacccagcat ggcggcggta atggtgcaga
      841 tgttggtcag ggctcagatg acagctcaat cgatctgacc caacgtggct tcggtaacag
      901 cgctactctt gatcagtgga acggcaaaaa ttctgaaatg acggttaaac agttcggtgg
      961 tggcaacggt gctgcagttg accagactgc atctaactcc tccgtcaacg tgactcaggt
     1021 tggctttggt aacaacgcga ccgctcatca gtactactat cgcaaataca aagagtacga
     1081 tgattggtaa acgcgtgcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1141 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     1201 ccctagacct agggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
     1261 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
     1321 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
     1381 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     1441 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
     1501 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
     1561 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     1621 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
     1681 aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc
     1741 gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta
     1801 cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt
     1861 ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt
     1921 attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa tgtagcacct
     1981 gaagtcagcc ccatacgata taagttgtta ctagtgcttg gattctcacc aataaaaaac
     2041 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     2101 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     2161 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     2221 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     2281 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     2341 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     2401 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     2461 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     2521 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa
     2581 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     2641 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     2701 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggat
//
