LOCUS       sequence-199065-        2758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conductive Curli Protein csgAY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Kalyoncu E, Ahan RE, Olmez TT, Seker UOS
  TITLE     Genetically encoded conductive protein nanofibers secreted by 
            engineered cells
  JOURNAL   RSC Adv. 2017, Issue 52. doi: 10.1039/C7RA06289C
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RSC Adv. 
            2017, Issue 52. doi: 10.1039/C7RA06289C"
COMMENT     Sequence Label: pZa-tetO-csgAY-CmR
FEATURES             Location/Qualifiers
     source          1..2758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(6..31,32..37,38..54,55..60,61..79))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    36..54
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    61..79
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    332..350
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    357..375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        414..516
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             517..1176
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(583..602)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1197..1291
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1405..1950
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1851..1870
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2067..2084
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2119..2205)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             2718..2729
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aattcggtca gtgcgtcctg ctgatgtgct cagtatctct atcactgata gggatgtcaa
       61 tctctatcac tgatagggac tcgagaaggc ccagtctttc gactgagcct ttcgttttat
      121 ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac
      181 ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag
      241 gcatctagag atatatggta gtagtaagtt aattttcatt aaccaccact accaatcacc
      301 tcctggattt gggtgtcgac tgtgctcagt atctctatca ctgataggga tgtcaatctc
      361 tatcactgat agggagggcc ctttgacgtc gatatctggc gaaaatgaga cgttgatcgg
      421 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt
      481 gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
      541 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
      601 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
      661 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
      721 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
      781 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
      841 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
      901 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
      961 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     1021 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     1081 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     1141 caacagtact gcgatgagtg gcagggcggg gcgtaatttg atatcgagct cgcttggact
     1201 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
     1261 ttgccgccgg gcgtttttta ttggtgagaa tccaagcact agtaacaact tatatcgtat
     1321 ggggctgact tcaggtgcta catttgaaga gataaattgc actgaaatct agaaatattt
     1381 tatctgatta ataagatgat cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc
     1441 tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag gttctctgag ctaccaactc
     1501 tttgaaccga ggtaactggc ttggaggagc gcagtcacca aaacttgtcc tttcagttta
     1561 gccttaaccg gcgcatgact tcaagactaa ctcctctaaa tcaattacca gtggctgctg
     1621 ccagtggtgc ttttgcatgt ctttccgggt tggactcaag acgatagtta ccggataagg
     1681 cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
     1741 acccggaact gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca
     1801 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccgccagg gggaaacgcc
     1861 tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga
     1921 tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct ctcacttccc
     1981 tgttaagtat cttcctggca tcttccagga aatctccgcc ccgttcgtaa gccatttccg
     2041 ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc
     2101 ctaggtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     2161 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tctagcacgc
     2221 gtttagtagt catcatactc tttgtatttg cgatagtagt actgatgagc ggtcgcgttg
     2281 ttaccaaagc caacctgagt cacgttgacg gaggagttag atgcagtctg gtcaactgca
     2341 gcaccgttgc caccaccgaa ctgtttaacc gtcatttcag aatttttgcc gttccactga
     2401 tcaagagtag cgctgttacc gaagccacgt tgggtcagat cgattgagct gtcatctgag
     2461 ccctgaccaa catctgcacc attaccgccg ccatgctggg taatagtcaa gtcagagtta
     2521 cgggcatcag tttgcagagc aagtgcagag ttaccgccac cgtactggta aatgttcagc
     2581 tcagaatttg ggccgctatt attaccgcca ccaccgtggt taccgccgcc gccgtactga
     2641 ggaacaacac ctgccagagc gctaccggag aatacgattg ctgcaattgc tgctactttt
     2701 aaaagtttca tggtaccttt ctcctcttta atacccaaat ccaagaggtg aatggtag
//
