LOCUS       sequence-199083-        6291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV expression of GluSnFR, a glutamate sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6291)
  TITLE     Baljit Khakh lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV.GfaABC1D.GluSnFr.SV40
FEATURES             Location/Qualifiers
     source          1..6291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   38..167
                     /label=AAV2 ITR
     intron          1018..1150
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1133..1152
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1195..1213
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     1248..1307
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     2276..2297
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2403..2424)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2664..2683)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2889..2918
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2922..3068
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     polyA_signal    3095..3216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3106..3125)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3160..3179
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3378..3507
                     /label=AAV2 ITR
     primer_bind     complement(3526..3543)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3526..3542)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3535..3557)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3684..4139
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3771..3790)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3981..4002
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4165..4269
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4270..4338,4339..5130)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5301..5889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5790..5809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6177..6198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6213..6218,6219..6236,6237..6243)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6251..6267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6256..6278
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6275..6291
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6275..6291
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgattacg ccagatttaa ttaaggcctt aattaggctg cgcgctcgct cgctcactga
       61 ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct cagtgagcga
      121 gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt agttaatgat
      181 taacccgcca tgctacttat ctacgtagcc atgctctagg aagatctaac atatcctggt
      241 gtggagtagg ggacgctgct ctgacagagg ctcgggggcc tgagctggct ctgtgagctg
      301 gggaggaggc agacagccag gccttgtctg caagcagacc tggcagcatt gggctggccg
      361 ccccccaggg cctcctcttc atgcccagtg aatgactcac cttggcacag acacaatgtt
      421 cggggtgggc acagtgcctg cttcccgccg caccccagcc cccctcaaat gccttccgag
      481 aagcccattg agcagggggc ttgcattgca ccccagcctg acagcctggc atcttgggat
      541 aaaagcagca cagcccccta ggggctgccc ttgctgtgtg gcgccaccgg cggtggagaa
      601 caaggctcta ttcagcctgt gcccaggaaa ggggatcagg ggatgcccag gcatggacag
      661 tgggtggcag ggggggagag gagggctgtc tgcttcccag aagtccaagg acacaaatgg
      721 gtgaggggag agctctcccc atagctgggc tgcggcccaa ccccaccccc tcaggctatg
      781 ccagggggtg ttgccagggg cacccgggca tcgccagtct agcccactcc ttcataaagc
      841 cctcgcatcc caggagcgag cagagccaga gcaggttgga gaggagacgc atcacctccg
      901 ctgctcgcaa gctttattgc ggtagtttat cacagttaaa ttgctaacgc agtcagtgct
      961 tctgacacaa cagtctcgaa cttaagctgc agaagttggt cgtgaggcac tgggcaggta
     1021 agtatcaagg ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag
     1081 agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt
     1141 ctctccacag gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac
     1201 gactcactat aggctagcct cgagaattcg gcttggggat atccaccatg gagacagaca
     1261 cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac agatctgccg
     1321 caggcagcac gctggacaaa atcgccaaaa acggtgtgat tgtcgtcggt caccgtgaat
     1381 cttcagtgcc tttctcttat tacgacaatc agcaaaaagt ggtgggttac tcgcaggatt
     1441 actccaacgc cattgttgaa gcagtgaaaa agaaactcaa caaaccggac ttgcaggtaa
     1501 aactgattcc gattacctca caaaaccgta ttccactgct gcaaaacggc actttcgatt
     1561 ttgaatgtgg ttctaccacc aacaacgtcg aacgccaaaa acaggcggct ttctctgaca
     1621 ctattttcgt ggtcggtacg cgcctgttga ccaaaaaggg tggcgatatc aaagattttg
     1681 ccaacctgaa agacaaagcc gtagtcgtca cttccggcac tacctctgaa gttttgctca
     1741 acaaactgaa tgaagagcaa aaaatgaata tgcgcatcat cagcgccaaa gatcacggtg
     1801 actctttccg caccctggaa agcggtcgtg ccgttgcctt tatgatggat gacgtgctgc
     1861 tggccggtga acgtgcgaaa gcgaagaaac cagacaactg ggaaatcgtc ggcaagccgc
     1921 agtctcagga ggcctacggt tgtatgttgc gtaaagatga tccgcagttc aaaaagctga
     1981 tggatgacac catcgctcag gtgcagacct ccggtgaagc ggaaaaatgg tttgataagt
     2041 ggttcaaaaa tccaattctg gtgagccaca acgtctatat catggccgac aagcagagga
     2101 acggcatcaa ggcgaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg
     2161 cctaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2221 actacctgag cacccagtcc aaactttcga aagaccccaa cgagaagcgc gatcacatgg
     2281 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     2341 gcggtaccgg agggagcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     2401 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg
     2461 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     2521 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     2581 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacatcc
     2641 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     2701 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     2761 gcaacatcct ggggcataag ctggagtaca actttaacaa ccccctgaac atgaatttcg
     2821 aactgtcaga cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaaactgc
     2881 aggtcgacga acaaaaactc atctcagaag aggatctgaa tgctgtgggc caggacacgc
     2941 aggaggtcat cgtggtgcca cactccttgc cctttaaggt ggtggtgatc tcagccatcc
     3001 tggccctggt ggtgctcacc atcatctccc ttatcatcct catcatgctt tggcagaaga
     3061 agccacgtta ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     3121 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3181 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     3241 atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     3301 tgtggtaaaa tcgataagga tcttcctaga gcatggctac gtagataagt agcatggcgg
     3361 gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     3421 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     3481 ggcctcagtg agcgagcgag cgcgcagcct taattaacct aattcactgg ccgtcgtttt
     3541 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3601 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3661 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3721 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3781 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3841 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     3901 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3961 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4021 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4081 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     4141 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4201 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4261 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4321 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4381 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4441 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4501 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4561 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4621 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4681 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4741 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4801 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4861 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4921 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4981 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5041 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5101 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5161 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5221 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5341 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5401 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5461 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5521 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5581 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5641 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5701 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5761 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5821 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5881 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5941 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6001 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6061 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6121 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     6181 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     6241 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga c
//
