LOCUS       sequence-199094-        5507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E.coli/H.Pylori shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Heuermann D, Haas R
  TITLE     A stable shuttle vector system for efficient genetic complementation
            of Helicobacter pylori strains by transformation and conjugation.
  JOURNAL   Mol Gen Genet. 1998 Mar;257(5):519-28.
  PUBMED    9563837
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Gen 
            Genet."; date: "1998-03"; volume: "257(5)"; pages: "519-28"
COMMENT     Sequence Label: pHel3
FEATURES             Location/Qualifiers
     source          1..5507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2304..3098)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      complement(3868..4456)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3948..3967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            4682..4791
                     /note="incP origin of transfer"
ORIGIN
        1 catttttgat agtagtgatt ttagtggtaa tcttgacaca gccactatat taaaacctta
       61 gcgttttaat aacccttata agtccgccaa gacttcttaa gggtttcact cctgttatta
      121 tatcgtcttt tgaaaaataa gcattaaaag gcgcttaaat gcccatgaat acgaattttg
      181 aacagcttag aaaacaagaa ttggaattac gaaaattatt agaagaatta gaaacgctcc
      241 cacaaacccc acaaattaaa ctgcaaaaac aaaaaataca aacttacata gacaagataa
      301 caccaagtat tttgagcggt tttgatcaaa aattcaaaga aattatagaa aatctatcaa
      361 atgaatttga aaaagaaaaa tccacaccac tcaaagagcc acaaaccacc cccacaccat
      421 gcaaagattt agtggttagc acccctaaag ataacaccta taccacctac cacaataacg
      481 ctaataaggt caatctaggg aaattgagcg aaagggaagc caatctttta ttcgctattt
      541 ttcaaaaact caaagatcaa gggaataccc tcattcgttt tgaaccgcaa gatttgaaac
      601 gcatgatcat agtcaaatcc aacctaacca acagacaatt attgcaaatc ctcaaaaact
      661 tacttgacaa cataggcggt gctaattttt ggatcattag agagcatgtt gaaaatggcg
      721 aaatctatga agatcacact agctacatgc ttttcaaaca atttgacatt cgtatccata
      781 agccaaccca aactatagaa tacttagaag tccaactcaa cgatagctac cactacctgc
      841 tcaacaatct aggcatgagc ggtcaataca cttcctttaa acttttagaa ttccaaaggg
      901 tgaggggcaa atacgccaag acgctctatc gcttactcaa gcaatacaaa agcacaggga
      961 ttttgagcgt ggaatgggat caattcaggg agcttttaga cattccaaaa gattacaaaa
     1021 tggaaaacat cgatcaaaaa gtcttaactc caagcctcaa ggaactcaga aaaatctatc
     1081 cctttgaaca cttgagctac aaaaaagaac gcaaaagcca cgacaaacgc aaggtaacgc
     1141 atattgattt ttattttgag caattgccac agggcgaaac caagaaacaa aaacaagccg
     1201 acaagcaacg cgctcaaagg gacatcaagc tcatagcgtg ggatattcac aaccaaatcg
     1261 ctaaaagaaa cgctaaagcc actatagaag ctaggtttct tgagctaaaa accttgattg
     1321 gttatcagtt caggcacaac aatgggacta ttttacagat tgacaacgcc acttttgaaa
     1381 agaatcaaat gtttttgcat gtttcaacca gcaaaaattc tcaaaaattc cttgtgtcca
     1441 acaaaacatt cgctttagaa cttctgtttg tcaatggata cagcctaaaa aaagacatgt
     1501 tggaaattga tcccccaagt atccacccca tcaccaacga acctatcaag gaatttgcag
     1561 aatacatcgg aaaaacgatc cacatcacca atttcaatgt ggatcaatgc cctgagggga
     1621 tcaataacta cctgaaaatc actaggattg tgaaactgaa tgacaatcgg atctgtgttt
     1681 cagtccaaga tgtggataag cctgagaaac ttctaaaacc tttcattgct aaagatgaaa
     1741 aacatttgaa aaattggttt aagaaacact atcggtgaaa aataagtggt gggattaaca
     1801 atcgtccccc acaccttgca atgtagttag ttttgcatta ttttttctat aatctctatc
     1861 aattctgaga gaacatagat taattcaatg aaatttttca ttatctattt cctttctgta
     1921 aattttgtta aatatttatt aaaaaaaaat ttaaagatca ctctttaagt ggaacataca
     1981 aaggctgtag agagtgccac attaagcctg atgttttgct tgtgtataga gtgaaaggca
     2041 atattttaac tttagttagg cttggcagtc atagtgaatt gttttagaca aatttctttc
     2101 taaaatcgcc cagaatgaat ttttaattct aatttaatat aattcatcgg ttttcactca
     2161 aaacgatgaa atagggctat ttttagctca ttattggggt attttgtttt tagttgaaag
     2221 ctaacttcaa aattcaaaaa aacttcattt ccctttctag aggatcgatc cccggggatc
     2281 ttttagacat ctaaatctag gtactaaaac aattcatcca gtaaaatata atattttatt
     2341 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
     2401 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
     2461 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
     2521 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
     2581 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
     2641 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
     2701 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
     2761 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
     2821 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
     2881 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
     2941 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccaac
     3001 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
     3061 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc
     3121 tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt
     3181 tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt
     3241 tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatga tagaatttac
     3301 aagctataag gttattgtcc tgggtttcaa gcattagtcc atgcaagttt ttatgctttg
     3361 cccattctat agatatattg ataagcgcgc tgcctatgcc ttgccccctg aaatccttac
     3421 atacggcgat atcttctata taagcgtacc ggttccaatt ttttcgcagt ttaacttttc
     3481 cgacgcattt atcgtcttgg tagcttttta aatatggcgc ttcatagagt aattctgtaa
     3541 aggtccaatt ctcgttttca tacctcggta taatcttacc tatcacctca aatggttcgc
     3601 tgggtttgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc aggcatgcaa
     3661 gctagctttc gcgagctcga gatctagata tcgatgaatt caaaaaaaaa ggctccaaaa
     3721 ggagccttta atgatctaat tcgtatcagc tcactcaaag gcggtaatac ggttatccac
     3781 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3841 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3901 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3961 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4021 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4081 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4141 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4201 cttatcgcca ccggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4261 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     4321 tatctgcgct ctgctgaaac cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4381 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4441 aaaaaaagga tctcaagaag atcctttgat ctctgcagtt cagtaatttc ctgcatttgc
     4501 ctgtttccag tcggtagata ttccacaaaa cagcagggaa gcagcgcttt tccgctgcat
     4561 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat
     4621 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc
     4681 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt
     4741 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg
     4801 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gctgcagaag cttgtccgtt
     4861 agcaaatata atgtgtttag cacctaaaag cgcatctatc ttgtgttagc catctaacct
     4921 aaacaaaacc gcctagcgag cgttagcgag catggacaaa agcgcatcgc agtttgaaat
     4981 cgtaggcgtt agccggagat ggttttgcgt tagcaaaatc aaacaagata gcgcaaaccg
     5041 aggcgtcagc caaaaaaacc ctaaaactaa aatccccaaa atatgtagcg cgtcatgcgc
     5101 gttgtttata ttacatgttt taataactat gttgttttta catgttttta caacgcgcgc
     5161 gcgtgtgagg gattgggggt tgcaaccccc taaataacga agctgtaggg tttctcattt
     5221 ttgggttaaa atgaataaaa cagaacttct tacgagtgat aacagaactt cttacgagtg
     5281 ataacagaac ttcttacgag tgataacaga acttcttacg agtgataaca gaacttcttt
     5341 attgtgaagt tatgattatt aacaattttt agctaaaata acaacctaag gcggtgtaac
     5401 atggttgcta atcctagtgt taacaaattt ggagcaatta gctttaaaag ctagtgggtt
     5461 gggagtttgt agcgggtagc actccgttag gaggcacacc atgaaag
//
