LOCUS       sequence-199155-       12622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses RanCas13b in mammalian cells for knockdown of target RNAs 
            in combination with compatible guide RNAs. Localized to the 
            cytoplasm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12622)
  AUTHORS   Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung 
            J, Zhang F
  TITLE     RNA editing with CRISPR-Cas13.
  JOURNAL   Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
  PUBMED    29070703
REFERENCE   2  (bases 1 to 12622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180."
COMMENT     Sequence Label: pC0044-EF1a-RanCas13b-NES-mapk
FEATURES             Location/Qualifiers
     source          1..12622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      51..186
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     113..132
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        248..295
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             314..688
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    818..939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(855..874)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     909..928
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(988..1004)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(988..1004)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1001..1023)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1012..1028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1036..1042,1043..1060,1061..1066))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1081..1102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1219..1236)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1390..1978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1470..1489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2149..2940,2941..3009))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2772..2791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3010..3114)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3189..3208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3380..3759
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3761..3959
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3914..3934
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3977..4157
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4204..4329
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4822..5055
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5240..5284
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5582..5699
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5824..7002
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6055..6993
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6950..6970
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7009..7018
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10348..10428
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    10456..11044
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10509..10529)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10927..10938)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11047..11063)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11048..11064)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11569..11749
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11775..11792)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11781..12005
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12051..12479
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12138..12157)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12348..12369
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12488..12508)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(12493..12622,1..200)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
       61 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
      121 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
      181 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
      241 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
      301 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
      361 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
      421 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
      481 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
      541 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
      601 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
      661 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
      721 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
      781 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
      841 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      901 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
      961 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     1021 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1081 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1141 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1201 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1261 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1321 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1381 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1441 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1501 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1561 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1621 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1681 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1741 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1801 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1861 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1921 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1981 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2041 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2101 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2281 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3121 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     3181 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     3241 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     3301 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     3361 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     3421 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     3481 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     3541 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     3601 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     3661 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     3721 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     3781 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3841 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     3901 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
     3961 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     4021 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     4081 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     4141 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     4201 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     4261 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     4321 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     4381 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     4441 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     4501 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     4561 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     4621 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     4681 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     4741 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     4801 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     4861 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     4921 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     4981 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     5041 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     5101 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     5161 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     5221 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     5281 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     5341 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     5401 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     5461 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     5521 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
     5581 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     5641 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     5701 gggtttatta cagggacagc agagatccag tttggttaat taatgcaaag atggataaag
     5761 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga
     5821 attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     5881 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     5941 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     6001 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     6061 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     6121 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     6181 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     6241 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     6301 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     6361 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
     6421 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     6481 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     6541 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     6601 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     6661 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     6721 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     6781 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     6841 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     6901 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     6961 acagtggttc aaagtttttt tcttccattt caggtgtcgt gacgtacggc caccatggag
     7021 aagcccctgc tgcctaacgt gtataccctg aagcacaagt tcttttgggg cgccttcctg
     7081 aatatcgccc ggcacaacgc ctttatcacc atctgccaca tcaatgagca gctgggcctg
     7141 aaaaccccca gcaacgacga taagatcgtg gacgtggtgt gcgagacatg gaacaatatc
     7201 ctgaacaatg accacgatct gctgaagaag tcccagctga ccgagctgat cctgaagcac
     7261 ttcccttttc tgacagccat gtgctaccac ccccctaaga aggagggcaa gaagaagggc
     7321 caccagaagg agcagcagaa ggagaaggag agcgaggcac agtcccaggc agaggccctg
     7381 aacccatcca agctgatcga ggccctggag atcctggtga atcagctgca ctctctgcgg
     7441 aactactata gccactataa gcacaagaag cccgacgccg agaaggatat cttcaagcac
     7501 ctgtataagg ccttcgacgc ctctctgaga atggtgaagg aggattataa ggcccacttc
     7561 accgtgaatc tgacaaggga ctttgcccac ctgaaccgca agggcaagaa caagcaggac
     7621 aatcccgact tcaacagata cagattcgag aaggatggct tctttaccga gtctggcctg
     7681 ctgttcttta caaatctgtt tctggacaag agggatgcct attggatgct gaagaaggtg
     7741 tccggcttca aggcctctca caagcagcgc gagaagatga ccacagaggt gttttgcagg
     7801 agccgcatcc tgctgcccaa gctgaggctg gagtcccgct acgaccacaa ccagatgctg
     7861 ctggatatgc tgtctgagct gagcagatgt cctaagctgc tgtatgagaa gctgagcgag
     7921 gagaataaga agcacttcca ggtggaggcc gacggctttc tggatgagat cgaggaggag
     7981 cagaacccat tcaaggacac cctgatccgg caccaggata gattccccta ctttgccctg
     8041 aggtatctgg acctgaatga gtccttcaag tctatccgct ttcaggtgga tctgggcaca
     8101 taccactatt gtatctacga caagaagatc ggcgatgagc aggagaagag gcacctgacc
     8161 cgcacactgc tgtccttcgg ccggctgcag gactttaccg agatcaacag accccaggag
     8221 tggaaggccc tgaccaagga cctggattac aaggagacat ccaatcagcc tttcatctct
     8281 aagaccacac cacactatca catcaccgac aacaagatcg gctttcggct gggcacaagc
     8341 aaggagctgt acccctccct ggagatcaag gatggcgcca atagaatcgc caagtaccct
     8401 tataactccg gcttcgtggc ccacgccttt atctctgtgc acgagctgct gcctctgatg
     8461 ttctaccagc acctgaccgg caagagcgag gacctgctga aggagacagt gcggcacatc
     8521 cagagaatct ataaggactt cgaggaggag cggatcaata ccatcgagga tctggagaag
     8581 gcaaaccagg gcagactgcc actgggagcc tttcccaagc agatgctggg cctgctgcag
     8641 aataagcagc ctgatctgtc cgagaaggcc aagatcaaga tcgagaagct gatcgccgag
     8701 acaaagctgc tgtctcacag gctgaacaca aagctgaaga gctccccaaa gctgggcaag
     8761 cggagagaga agctgatcaa gacaggcgtg ctggccgact ggctggtgaa ggatttcatg
     8821 cgctttcagc ccgtggccta cgacgcccag aatcagccta tcaagtctag caaggccaac
     8881 tccaccgagt tctggtttat caggcgcgcc ctggccctgt acggaggaga gaagaatagg
     8941 ctggagggct atttcaagca gaccaacctg atcggcaaca caaatccaca ccccttcctg
     9001 aacaagttta attggaaggc ctgcaggaat ctggtggatt tctaccagca gtatctggag
     9061 cagcgcgaga agtttctgga ggccatcaag aaccagccat gggagcccta ccagtattgc
     9121 ctgctgctga agatccctaa ggagaacagg aagaatctgg tgaagggatg ggagcaggga
     9181 ggaatctctc tgccacgggg cctgtttacc gaggccatca gagagacact gtctgaggac
     9241 ctgatgctga gcaagccaat ccgcaaggag atcaagaagc acggccgggt gggcttcatc
     9301 agcagagcca tcaccctgta ctttaaggag aagtatcagg ataagcacca gagcttctac
     9361 aatctgtcct ataagctgga ggcaaaggca ccactgctga agcgggagga gcactacgag
     9421 tattggcagc agaacaagcc tcagtctcca accgagagcc agaggctgga gctgcacaca
     9481 agcgaccgct ggaaggatta cctgctgtat aagagatggc agcacctgga gaagaagctg
     9541 cggctgtacc ggaatcagga cgtgatgctg tggctgatga ccctggagct gacaaagaac
     9601 cacttcaagg agctgaacct gaattatcac cagctgaagc tggagaatct ggccgtgaac
     9661 gtgcaggagg ccgatgccaa gctgaaccct ctgaatcaga ccctgcccat ggtgctgcct
     9721 gtgaaggtgt accctgccac agcctttggc gaggtgcagt accacaagac cccaatcagg
     9781 acagtgtata tccgcgagga gcacaccaag gccctgaaga tgggcaactt caaggccctg
     9841 gtgaaggacc ggagactgaa tggcctgttc tcttttatca aggaggagaa cgatacacag
     9901 aagcacccaa tcagccagct gcggctgagg cgcgagctgg agatctacca gtctctgaga
     9961 gtggacgcct tcaaggagac actgagcctg gaggagaagc tgctgaataa gcacacatcc
    10021 ctgtcctctc tggagaacga gtttagggcc ctgctggagg agtggaagaa ggagtatgcc
    10081 gccagctcca tggtgacaga cgagcacatc gccttcatcg ccagcgtgcg caatgccttc
    10141 tgccacaacc agtacccctt ctacaaggag gccctgcacg cccctatccc actgttcacc
    10201 gtggcccagc ccaccacaga ggagaaggat ggcctgggaa tcgcagaggc cctgctgaag
    10261 gtgctgaggg agtactgtga gatcgtgaag tcccagatcg gatccagcct ccaaaaaaag
    10321 ttggaggaat tagaactagg tagttcctac ccatacgatg ttccagatta cgcttatccc
    10381 tacgacgtgc ctgattatgc atacccatat gatgtccccg actatgccta agaattcgat
    10441 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
    10501 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
    10561 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
    10621 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
    10681 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
    10741 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
    10801 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
    10861 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
    10921 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
    10981 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
    11041 ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
    11101 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
    11161 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
    11221 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
    11281 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
    11341 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
    11401 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
    11461 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
    11521 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
    11581 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
    11641 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    11701 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
    11761 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
    11821 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
    11881 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
    11941 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
    12001 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
    12061 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
    12121 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
    12181 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
    12241 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
    12301 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
    12361 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
    12421 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
    12481 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
    12541 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
    12601 ccagcaggca gaagtatgca aa
//
