LOCUS       sequence-199158-        2552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mini-transposon system suitable for sequencing, shuttle mutagenesis 
            and gene fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Kahrs AF, Odenbreit S, Schmitt W, Heuermann D, Meyer TF, Haas R
  TITLE     An improved TnMax mini-transposon system suitable for sequencing, 
            shuttle mutagenesis and gene fusions.
  JOURNAL   Gene. 1995 Dec 29;167(1-2):53-7.
  PUBMED    8566811
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1995-12-29"; volume: "167(1-2)"; pages: "53-7"
COMMENT     Sequence Label: pMin1
FEATURES             Location/Qualifiers
     source          1..2552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             186..1376
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(538..557)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            1600..1709
                     /note="incP origin of transfer"
     rep_origin      1797..2385
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2286..2305
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aattcagatc tcgaggtacc cgggattctc tagatcgatg tcgacaagat tgatgtcgat
       61 aagtttagcc cgcctaatga gcgggctttt tttttgatct aattcatatt tgacattgcc
      121 atatagatga gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac
      181 cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg
      241 taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg
      301 acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat
      361 gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt
      421 cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc
      481 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      541 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
      601 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
      661 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
      721 tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc
      781 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
      841 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
      901 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
      961 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     1021 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     1081 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     1141 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     1201 cgctcgcggc tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt
     1261 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
     1321 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg
     1381 aagctggcgg ctgcagttca gtaatttcct gcatttgcct gtttccagtc ggtagatatt
     1441 ccacaaaaca gcagggaagc agcgcttttc cgctgcataa ccctgcttcg gggtcattat
     1501 agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt
     1561 tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg
     1621 cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa
     1681 cgggaatcct gctctgcgag gctggccggc taccgccggc gtaacagatg agggcaagcg
     1741 gatggctgat gaaaccaagc tgcagagctt agctctgcag agatcaaagg atcttcttga
     1801 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1861 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1921 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1981 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2041 ggtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2101 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2161 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2221 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2281 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2341 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2401 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2461 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac gaattagatc
     2521 attaaaggct ccttttggag cctttttttt tg
//
