LOCUS       sequence-199164-        1221 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abu-Remaileh M, Wyant GA, Kim C, Laqtom NN, Abbasi M, Chan SH, 
            Freinkman E, Sabatini DM
  TITLE     Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent 
            regulation of amino acid efflux from lysosomes.
  JOURNAL   Science. 2017 Nov 10;358(6364):807-813. doi: 
            10.1126/science.aan6298. Epub 2017 Oct 26.
  PUBMED    29074583
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aan6298"; journalName: "Science"; date: 
            "2017-11-10- 10"; volume: "358"; issue: "6364"; pages: "807-813"
COMMENT     Sequence Label: pLJC5-LAMP1-RFP-2xFlag
FEATURES             Location/Qualifiers
     source          1..1221
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..63,64..1221)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
ORIGIN
        1 atggcggccc cgggcgcccg gcggccgctg ctcctgttgc tgctggcagg ccttgcacac
       61 agcgccccag cactgttcga ggtgaaagac aacaacggca cagcgtgtat aatggccagc
      121 ttctctgcct cctttctgac cacctatgag gctggacatg tttctaaggt ctcgaatatg
      181 accctgccag cctctgcaga agtcctgaag aatagcagct cttgtggtga aaagaatgct
      241 tctgagccca ccctcgcaat cacctttgga gaaggatatt tactgaaact caccttcaca
      301 aaaaacacaa cacgttacag tgtccagcac atgtatttca catataacct gtcagacaca
      361 caattctttc ccaatgccag ctccaaaggg cccgacactg tggattccac aactgacatc
      421 aaggcagaca tcaacaaaac ataccgatgt gtcagcgaca tcagggtcta catgaagaat
      481 gtgaccattg tgctctggga cgctactatc caggcctacc tgccgagtag caacttcagc
      541 aaggaagaga cacgctgccc acaggatcaa ccttccccaa ctactgggcc acccagcccc
      601 tcaccaccac ttgtgcccac aaaccccagt gtgtccaagt acaatgtgac tggtgacaat
      661 ggaacctgcc tgctggcctc tatggcactg caactcaaca tcacctacat gaagaaggac
      721 aacacgactg tgaccagagc attcaacatc aacccaagtg acaaatatag tgggacttgc
      781 ggtgcccagt tggtgaccct gaaggtgggg aacaagagca gagtcctgga gctgcagttt
      841 gggatgaatg ccacttctag cctgtttttc ctgcaaggag ttcagttgaa catgactctt
      901 cctgatgcca tagagcccac gttcagcacc tccaactatt ccctgaaagc tcttcaggcc
      961 agtgtcggca actcatacaa gtgcaacagt gaggagcaca tctttgtcag caaggcgctc
     1021 gccctcaatg tcttcagcgt gcaagtccag gctttcaggg tagaaagtga caggtttggg
     1081 tctgtggaag agtgtgtaca ggacggtaac aacatgctga tccccattgc tgtgggcggg
     1141 gccctggcag ggctggtcct catcgtcctc atcgcctacc tcatcggcag gaagaggagt
     1201 cacgcgggct atcagaccat c
//
