LOCUS       sequence-199165-        9962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Abu-Remaileh M, Wyant GA, Kim C, Laqtom NN, Abbasi M, Chan SH, 
            Freinkman E, Sabatini DM
  TITLE     Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent 
            regulation of amino acid efflux from lysosomes.
  JOURNAL   Science. 2017 Nov 10;358(6364):807-813. doi: 
            10.1126/science.aan6298. Epub 2017 Oct 26.
  PUBMED    29074583
REFERENCE   2  (bases 1 to 9962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aan6298"; journalName: "Science"; date: 
            "2017-11-10- 10"; volume: "358"; issue: "6364"; pages: "807-813"
COMMENT     Sequence Label: pLJC5-LAMP1-RFP-2xFlag
FEATURES             Location/Qualifiers
     source          1..9962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1824..3035
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2872..2891
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3057..3066
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3059..3121,3122..4279)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             4289..4963
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             4289..4963
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(4419..4437)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4687..4706
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4881..4904
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             4964..5011
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     misc_feature    5056..5173
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5222..5732
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5651..5669
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5754..6353
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5754..5773)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6250..6270
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6481..6714
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6786..6907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6823..6842)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6877..6896
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6947..7082
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7102..7121)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7103..7121)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7131..7148)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7131..7147)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7140..7162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7289..7744
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7376..7395)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7586..7607
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7770..7874
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7875..7943,7944..8735)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8093..8112)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8906..9494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9395..9414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9648..9665
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9782..9803
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9818..9823,9824..9841,9842..9848)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9856..9872
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9861..9883
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9880..9896
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9880..9896
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9915..9935
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9917..9935
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     1861 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     1921 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     1981 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2041 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2101 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2161 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2221 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2281 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2341 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2401 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2461 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2521 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2581 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2641 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2701 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     2761 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     2821 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     2881 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     2941 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3001 ctaaattctg gccgtttttg gcttttttgt tagacgaagc gctagcgcta ccggtaccat
     3061 ggcggccccg ggcgcccggc ggccgctgct cctgttgctg ctggcaggcc ttgcacacag
     3121 cgccccagca ctgttcgagg tgaaagacaa caacggcaca gcgtgtataa tggccagctt
     3181 ctctgcctcc tttctgacca cctatgaggc tggacatgtt tctaaggtct cgaatatgac
     3241 cctgccagcc tctgcagaag tcctgaagaa tagcagctct tgtggtgaaa agaatgcttc
     3301 tgagcccacc ctcgcaatca cctttggaga aggatattta ctgaaactca ccttcacaaa
     3361 aaacacaaca cgttacagtg tccagcacat gtatttcaca tataacctgt cagacacaca
     3421 attctttccc aatgccagct ccaaagggcc cgacactgtg gattccacaa ctgacatcaa
     3481 ggcagacatc aacaaaacat accgatgtgt cagcgacatc agggtctaca tgaagaatgt
     3541 gaccattgtg ctctgggacg ctactatcca ggcctacctg ccgagtagca acttcagcaa
     3601 ggaagagaca cgctgcccac aggatcaacc ttccccaact actgggccac ccagcccctc
     3661 accaccactt gtgcccacaa accccagtgt gtccaagtac aatgtgactg gtgacaatgg
     3721 aacctgcctg ctggcctcta tggcactgca actcaacatc acctacatga agaaggacaa
     3781 cacgactgtg accagagcat tcaacatcaa cccaagtgac aaatatagtg ggacttgcgg
     3841 tgcccagttg gtgaccctga aggtggggaa caagagcaga gtcctggagc tgcagtttgg
     3901 gatgaatgcc acttctagcc tgtttttcct gcaaggagtt cagttgaaca tgactcttcc
     3961 tgatgccata gagcccacgt tcagcacctc caactattcc ctgaaagctc ttcaggccag
     4021 tgtcggcaac tcatacaagt gcaacagtga ggagcacatc tttgtcagca aggcgctcgc
     4081 cctcaatgtc ttcagcgtgc aagtccaggc tttcagggta gaaagtgaca ggtttgggtc
     4141 tgtggaagag tgtgtacagg acggtaacaa catgctgatc cccattgctg tgggcggggc
     4201 cctggcaggg ctggtcctca tcgtcctcat cgcctacctc atcggcagga agaggagtca
     4261 cgcgggctat cagaccatca tggatccgat ggcctcctcc gaggacgtca tcaaggagtt
     4321 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     4381 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     4441 cggccccctg cccttcgcct gggacatcct gtcccctcag ttccagtacg gctccaaggc
     4501 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     4561 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     4621 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     4681 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcggatgta
     4741 ccccgaggac ggcgccctga agggcgagat caagatgagg ctgaagctga aggacggcgg
     4801 ccactacgac gccgaggtca agaccaccta catggccaag aagcccgtgc agctgcccgg
     4861 cgcctacaag accgacatca agctggacat cacctcccac aacgaggact acaccatcgt
     4921 ggaacagtac gagcgcgccg agggccgcca ctccaccggc gccgactaca aagacgatga
     4981 cgacaaggac tacaaagacg atgacgacaa gtgagaattc tcgacctcga gacaaatggc
     5041 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     5101 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     5161 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga
     5221 gggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     5281 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     5341 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     5401 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     5461 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
     5521 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
     5581 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
     5641 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
     5701 tcgttgaccg aatcaccgac ctctctcccc agggggatcc accggagctt accatgaccg
     5761 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     5821 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     5881 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     5941 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     6001 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     6061 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     6121 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     6181 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     6241 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     6301 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     6361 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg gtacctttaa
     6421 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6481 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     6541 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6601 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6661 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     6721 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6781 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6841 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6901 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     6961 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7021 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7081 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7141 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7201 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7261 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     7321 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7381 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7441 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7501 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7561 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7621 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7681 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     7741 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7801 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7861 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7921 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7981 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8041 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8101 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8161 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8221 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8281 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8341 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8401 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8461 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8521 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8581 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8641 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8701 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8761 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8821 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8881 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8941 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9001 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9061 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9121 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9181 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9241 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9301 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9361 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9421 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9481 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9541 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9601 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9661 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9721 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9781 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9841 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     9901 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     9961 aa
//
