LOCUS       sequence-199166-       10031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Abu-Remaileh M, Wyant GA, Kim C, Laqtom NN, Abbasi M, Chan SH, 
            Freinkman E, Sabatini DM
  TITLE     Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent 
            regulation of amino acid efflux from lysosomes.
  JOURNAL   Science. 2017 Nov 10;358(6364):807-813. doi: 
            10.1126/science.aan6298. Epub 2017 Oct 26.
  PUBMED    29074583
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aan6298"; journalName: "Science"; date: 
            "2017-11-10- 10"; volume: "358"; issue: "6364"; pages: "807-813"
COMMENT     Sequence Label: pLJC5-LAMP1-RFP-3xHA
FEATURES             Location/Qualifiers
     source          1..10031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1824..3035
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2872..2891
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3057..3066
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3059..3121,3122..4279)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             4289..4963
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             4289..4963
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(4419..4437)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4687..4706
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4881..4904
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             4964..4990
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5003..5029
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5042..5068
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5125..5242
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5291..5801
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5720..5738
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5823..6422
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5823..5842)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6319..6339
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6550..6783
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6855..6976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6892..6911)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6946..6965
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7016..7151
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7171..7190)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7172..7190)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7200..7217)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7200..7216)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7209..7231)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7358..7813
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7445..7464)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7655..7676
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7839..7943
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7944..8012,8013..8804)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8162..8181)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8975..9563
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9464..9483
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9717..9734
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9851..9872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9887..9892,9893..9910,9911..9917)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9925..9941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9930..9952
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9949..9965
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9949..9965
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9984..10004
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9986..10004
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     1861 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     1921 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     1981 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2041 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2101 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2161 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2221 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2281 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2341 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2401 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2461 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2521 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2581 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2641 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2701 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     2761 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     2821 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     2881 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     2941 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3001 ctaaattctg gccgtttttg gcttttttgt tagacgaagc gctagcgcta ccggtaccat
     3061 ggcggccccg ggcgcccggc ggccgctgct cctgttgctg ctggcaggcc ttgcacacag
     3121 cgccccagca ctgttcgagg tgaaagacaa caacggcaca gcgtgtataa tggccagctt
     3181 ctctgcctcc tttctgacca cctatgaggc tggacatgtt tctaaggtct cgaatatgac
     3241 cctgccagcc tctgcagaag tcctgaagaa tagcagctct tgtggtgaaa agaatgcttc
     3301 tgagcccacc ctcgcaatca cctttggaga aggatattta ctgaaactca ccttcacaaa
     3361 aaacacaaca cgttacagtg tccagcacat gtatttcaca tataacctgt cagacacaca
     3421 attctttccc aatgccagct ccaaagggcc cgacactgtg gattccacaa ctgacatcaa
     3481 ggcagacatc aacaaaacat accgatgtgt cagcgacatc agggtctaca tgaagaatgt
     3541 gaccattgtg ctctgggacg ctactatcca ggcctacctg ccgagtagca acttcagcaa
     3601 ggaagagaca cgctgcccac aggatcaacc ttccccaact actgggccac ccagcccctc
     3661 accaccactt gtgcccacaa accccagtgt gtccaagtac aatgtgactg gtgacaatgg
     3721 aacctgcctg ctggcctcta tggcactgca actcaacatc acctacatga agaaggacaa
     3781 cacgactgtg accagagcat tcaacatcaa cccaagtgac aaatatagtg ggacttgcgg
     3841 tgcccagttg gtgaccctga aggtggggaa caagagcaga gtcctggagc tgcagtttgg
     3901 gatgaatgcc acttctagcc tgtttttcct gcaaggagtt cagttgaaca tgactcttcc
     3961 tgatgccata gagcccacgt tcagcacctc caactattcc ctgaaagctc ttcaggccag
     4021 tgtcggcaac tcatacaagt gcaacagtga ggagcacatc tttgtcagca aggcgctcgc
     4081 cctcaatgtc ttcagcgtgc aagtccaggc tttcagggta gaaagtgaca ggtttgggtc
     4141 tgtggaagag tgtgtacagg acggtaacaa catgctgatc cccattgctg tgggcggggc
     4201 cctggcaggg ctggtcctca tcgtcctcat cgcctacctc atcggcagga agaggagtca
     4261 cgcgggctat cagaccatca tggatccgat ggcctcctcc gaggacgtca tcaaggagtt
     4321 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     4381 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     4441 cggccccctg cccttcgcct gggacatcct gtcccctcag ttccagtacg gctccaaggc
     4501 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     4561 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     4621 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     4681 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcggatgta
     4741 ccccgaggac ggcgccctga agggcgagat caagatgagg ctgaagctga aggacggcgg
     4801 ccactacgac gccgaggtca agaccaccta catggccaag aagcccgtgc agctgcccgg
     4861 cgcctacaag accgacatca agctggacat cacctcccac aacgaggact acaccatcgt
     4921 ggaacagtac gagcgcgccg agggccgcca ctccaccggc gcctatccct atgacgtgcc
     4981 tgattacgcc ggcacaggat cctaccccta tgatgtgcct gactacgctg gcagcgccgg
     5041 atacccttat gatgtgcctg attatgctgg agggagcggc taagaattct cgacctcgag
     5101 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     5161 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     5221 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc
     5281 gcggctcgag ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac
     5341 gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat
     5401 tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg
     5461 cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga
     5521 cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag
     5581 caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg
     5641 ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg
     5701 ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag
     5761 tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta
     5821 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     5881 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     5941 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     6001 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     6061 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     6121 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     6181 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     6241 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     6301 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     6361 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     6421 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg
     6481 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6541 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     6601 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6661 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6721 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6781 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     6841 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6901 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6961 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     7021 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7081 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7141 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     7201 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7261 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7321 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     7381 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7441 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7501 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7561 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7621 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7681 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7741 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     7801 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7861 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7921 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7981 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8041 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8101 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8161 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8221 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8281 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8341 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8401 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8461 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     8521 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     8581 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     8641 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     8701 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8761 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8821 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8881 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8941 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9001 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9061 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9121 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9181 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9301 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9361 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9421 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9481 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9541 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     9601 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     9661 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9721 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     9781 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     9841 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     9901 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     9961 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    10021 tgcaagctta a
//
