LOCUS       sequence-199186-        3649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Hartmann C, Ortiz de Montellano PR
  TITLE     Baculovirus expression and characterization of catalytically active 
            horseradish peroxidase.
  JOURNAL   Arch Biochem Biophys. 1992 Aug 15;297(1):61-72.
  PUBMED    1637184
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Biochem Biophys."; date: "1992-08-15"; volume: "297(1)"; pages: 
            "61-72"
COMMENT     Sequence Label: pUC19-HRP-C
FEATURES             Location/Qualifiers
     source          1..3649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(1202..1219)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1253..1270)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1253..1269)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1262..1284)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1478..1497)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1597..1619
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1657..1675)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1743..1847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1848..1916,1917..2708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2066..2085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2879..3467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3368..3387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3621..3638
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcctt attaagagtt gctgttgacc actctgcagt
      301 tcagacgaat ctggccttgg gtacccgtca gaggggtaat gttacccata cggtccatgg
      361 cttccacgaa ggcgttaaag aaggtttgag tagagttagc aaaacttctc accagtggga
      421 tggtgtcagt ggcgtttgga ctgctaaaca gttcttgatc actctgtatc aggcctttct
      481 gctcctctag attcacatag tacttgttat cgaagatggt tggggtccgc agatcaaagt
      541 ccactagtgc actgaggttg ccattcagtg ggcacaagcc tctcagtgtc tggagatacg
      601 tagtgttcag cgtggggtca ggtaacccag tgttgctgaa attgtagagc ctatccatga
      661 tgaacctaca ctggttcttt ccaaatgtgt gtcctccgga cagagccaca aggtcactcg
      721 agcgattcag acccacgttt ctaaagctat ccttcagctg gggcagggtg aagaatggag
      781 caggcaagtt ggcgttggcc agatctagga atgcctgtag ggagtcacgt cgaccgagcg
      841 gcactctcca ggacggtccg cctgcaagag tcacgctctg ttgcgcagct atagtcagca
      901 ggtctgcaca actgactgtt cgtgggcatg ctgactcaac ggcagccttc atgcgatcga
      961 tcactggaaa gcccctggcg ctgttagcgt tcccgaatgc atccttttca gtgcggaaac
     1021 tggtggtgtt gtccagtaat atgctagcgt cgcaaccatt cacgaagcag tcatggaagt
     1081 gcagacgtaa tattgaagca gcgatcctgg gatcggatct gagctcgttg acgattgtgt
     1141 cgcgaacgat gttggacacg ttgggacagc tattgtcgta gaatgtaggg gttaactgca
     1201 tatggtggtg gtggtggtgg gcccgggatc cccgggtacc gagctcgaat tcactggccg
     1261 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     1321 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     1381 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     1441 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     1501 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     1561 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     1621 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     1681 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     1741 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1801 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1861 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1921 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1981 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2041 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2101 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2161 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2221 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2281 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     2341 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     2401 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2461 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2521 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     2581 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2641 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2701 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2761 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2821 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2881 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2941 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3001 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3061 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3121 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3181 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3241 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3301 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3361 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3421 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3481 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3541 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3601 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaaga
//
