LOCUS       sequence-199202-        3896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gene cloning of human CD1A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Manolova V, Hirabayashi Y, Mori L, De Libero G
  TITLE     CD1a and CD1b surface expression is independent from de novo 
            synthesized glycosphingolipids.
  JOURNAL   Eur J Immunol. 2003 Jan;33(1):29-37.
  PUBMED    12594829
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Immunol."; date: "2003-01"; volume: "33(1)"; pages: "29-37"
COMMENT     Sequence Label: pHCD1A
FEATURES             Location/Qualifiers
     source          1..3896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1037..1053)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1037..1053)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1085..1104)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1086..1104)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1111..1128)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1111..1127)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1120..1142)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1269..1724
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1356..1375)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1566..1587
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1750..1854
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1855..1923,1924..2715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2073..2092)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2886..3474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3375..3394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3628..3645
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3762..3783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3798..3803,3804..3821,3822..3828)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3836..3852
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3841..3863
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3860..3876
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3860..3876
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga gatgctgttt
       61 ttgctacttc cattgttagc tgttctccca ggtgatggca atgcagacgg gctcaaggag
      121 cctctctcct tccatgtcat ctggatcgca tccttttaca accattcctg gaaacaaaat
      181 ctggtctcag gttggctgag tgatttgcag actcatacct gggacagcaa ttccagcacc
      241 atcgttttcc tgtggccctg gtccagggga aacttcagca atgaggagtg gaaggaactg
      301 gaaacattat tccgtatacg caccattcgg tcatttgagg gaattcgtag atacgcccat
      361 gaattgcagt ttgaatatcc ttttgagata caggtgacag gaggctgtga gctgcactct
      421 ggaaaggtct caggaagctt cttgcagtta gcttatcaag gatcagactt tgtgagcttc
      481 cagaacaatt catggttgcc atatccagtg gctgggaata tggccaagca tttctgcaaa
      541 gtgctcaatc agaatcagca tgaaaatgac ataacacaca atcttctcag tgacacctgc
      601 ccacgtttca tcttgggtct tcttgatgca ggaaaggcac atctccagcg gcaagtgaag
      661 cccgaggcct ggctgtccca tggccccagt cctggccctg gccatctgca gcttgtgtgc
      721 catgtctcag gattctaccc aaagcccgtg tgggtgatgt ggatgcgggg tgagcaggag
      781 cagcagggca ctcagcgagg ggacatcttg cccagtgctg atgggacatg gtatctccgc
      841 gcaaccctgg aggtggccgc tggggaggca gctgacctgt cctgtcgggt gaagcacagc
      901 agtctagagg gccaggacat cgtcctctac tgggagcatc acagttccgt gggcttcatc
      961 atcttggcgg tgatagtgcc tttacttctt ctgataggtc ttgcgctttg gttcaggaaa
     1021 cgctgtttct gttaaggatc cactagttct agagcggccg ccaccgcggt ggagctccaa
     1081 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     1141 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1201 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1261 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     1321 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1381 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     1441 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     1501 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     1561 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     1621 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     1681 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     1741 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1801 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1861 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1921 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1981 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2041 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2101 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2161 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2221 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2281 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2341 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2401 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2461 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2521 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2581 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2641 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2701 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2761 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2821 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2881 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2941 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3001 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3061 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3541 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3601 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3661 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3721 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     3781 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     3841 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcga
//
