LOCUS       sequence-199220-        9741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a 
            pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' 
            overhangs. TagBFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Arnoult N, Correia A, Ma J, Merlo A, Garcia-Gomez S, Maric M, 
            Tognetti M, Benner CW, Boulton SJ, Saghatelian A, Karlseder J
  TITLE     Regulation of DNA repair pathway choice in S and G2 phases by the 
            NHEJ inhibitor CYREN.
  JOURNAL   Nature. 2017 Sep 20;549(7673):548-552. doi: 10.1038/nature24023.
  PUBMED    28959974
REFERENCE   2  (bases 1 to 9741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2017-09-20- 20"; volume: "549"; issue: "7673"; 
            pages: "548-552"
COMMENT     Sequence Label: pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1
FEATURES             Location/Qualifiers
     source          1..9741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        271..346
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        396..607
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             670..1371
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        1393..1607
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     1528..1547
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        1637..1712
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        1745..2030
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        2032..2309
                     /label=chicken beta-actin promoter
     intron          2310..2537
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      2549..2558
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2561..2581
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2606..6706
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6707..6754
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(6782..6799)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6788..6995
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7004..7144
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7004..7133
                     /label=AAV2 ITR
     rep_origin      7219..7674
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7306..7325)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7516..7537
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7691..7710)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7810..7832
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7870..7888)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7956..8060
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8061..8129,8130..8921)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8279..8298)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9092..9680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9581..9600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg ggttggcagc gctgcccgcg gttttagagc tagaaatagc aagttaaaat
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttagcgcgtg
      361 cgccaattct gcagacatga atcaacctct ggattgggca gagcgcacat cgcccacagt
      421 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
      481 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      541 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      601 aacacagctc tggctaacta gagaacccac tgtagtgaac cgtcagatcg cctggagacg
      661 ccatccacga tgtccgagct gattaaggag aacatgcaca tgaagctgta catggagggc
      721 accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
      781 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
      841 ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc
      901 gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
      961 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     1021 aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca
     1081 ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga
     1141 aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca
     1201 tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac
     1261 agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca
     1321 gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaattg attttagcgc
     1381 gtgcgccaat tcgaacgctg acgtcatcaa cccgctccaa ggaatcgcgg gcccagtgtc
     1441 actaggcggg aacacccagc gcgcgtgcgc cctggcagga agatggctgt gagggacagg
     1501 ggagtggcgc cctgcaatat ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt
     1561 gaaatgtctt tggatttggg aatcttataa gttctgtatg agaccacgaa acaccgccat
     1621 ggagaccccg tcccaggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
     1681 atcaacttga aaaagtggca ccgagtcggt gctttttttg cagacaaatg gctctagagg
     1741 tacccgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     1801 cattgacgtc aatagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1861 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     1921 ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc ccagtacatg accttatggg
     1981 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     2041 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     2101 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc
     2161 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
     2221 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
     2281 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc
     2341 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
     2401 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagctgagc aagaggtaag
     2461 ggtttaaggg atggttggtt ggtggggtat taatgtttaa ttacctggag cacctgcctg
     2521 aaatcacttt ttttcaggtt ggaccggtgc caccatggcc ccaaagaaga agcggaaggt
     2581 cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg acatcggcac
     2641 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     2701 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     2761 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     2821 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     2881 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     2941 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     3001 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     3061 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     3121 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     3181 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     3241 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     3301 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     3361 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
     3421 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     3481 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     3541 caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac
     3601 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     3661 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     3721 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     3781 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca
     3841 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
     3901 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     3961 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     4021 caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga
     4081 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     4141 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     4201 aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt
     4261 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     4321 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     4381 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     4441 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     4501 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     4561 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     4621 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     4681 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     4741 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     4801 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     4861 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     4921 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     4981 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     5041 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     5101 gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga
     5161 caacaaggtg ctgaccagaa gcgacaaggc ccggggcaag agcgacaacg tgccctccga
     5221 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     5281 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     5341 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     5401 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     5461 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     5521 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     5581 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     5641 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     5701 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     5761 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     5821 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     5881 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
     5941 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     6001 cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     6061 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     6121 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     6181 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     6241 gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     6301 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     6361 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     6421 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     6481 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     6541 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg
     6601 gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     6661 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacaaaa ggccggcggc
     6721 cacgaaaaag gccggccagg caaaaaagaa aaagtaagaa ttcctagagc tcgctgatca
     6781 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6841 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6901 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6961 gaggattggg aagagaatag caggcatgct ggggagcggc cgcaggaacc cctagtgatg
     7021 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     7081 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     7141 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     7201 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7261 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     7321 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7381 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     7441 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7501 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     7561 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     7621 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     7681 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     7741 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     7801 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     7861 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7921 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7981 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8041 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8101 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8161 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8221 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8281 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8341 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8401 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8461 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8521 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8581 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8641 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8701 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8761 gagccggtga gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8821 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8881 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8941 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     9001 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9061 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9121 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9181 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9241 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9301 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9361 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9421 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9481 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9541 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9601 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9661 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9721 gctggccttt tgctcacatg t
//
