LOCUS       sequence-199224-        6092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains: complete oriC region of M. florum L1 (oriC4 fragment), 
            colE1 rep origin, recoded tetM and cat resistance genes, origin of 
            transfer of RP4 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Matteau D, Pepin ME, Baby V, Gauthier S, Arango Giraldo M, Knight 
            TF, Rodrigue S
  TITLE     Development of oriC-Based Plasmids for Mesoplasma florum.
  JOURNAL   Appl Environ Microbiol. 2017 Mar 17;83(7). pii: e03374-16. doi: 
            10.1128/AEM.03374-16. Print 2017 Apr 1.
  PUBMED    28115382
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2017-03-17"; volume: "83(7). pii: 
            e03374-16. doi"; pages: " 10.1128/AEM.03374-16. Print 2017 Apr 1"
COMMENT     Sequence Label: pMflCT-o4
FEATURES             Location/Qualifiers
     source          1..6092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3086..3105
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3086..3104
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3112..3129
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     rep_origin      complement(3212..3800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3292..3311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3806..3824
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     oriT            3882..3991
                     /note="incP origin of transfer"
ORIGIN
        1 tccctttagt gagggttaat gtcgtgactg ggaaaaccct ggcggaattc ggccagtcta
       61 catgtactct ttttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat
      121 tatttaagtg actatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac
      181 tgtcaatttg atagcgggaa caaataatta gatgtccttt tttaggaggg cttagttttt
      241 tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccagag aatatctgta
      301 tgctttgtat acctatggtt atgcataaaa atcccggtga taaaagtatt tatcactggg
      361 atttttatgc ccttttgggt ttttgaatgg aggaaatcta gatgaaaatc ataaatatcg
      421 gtgtattagc tcacgttgat gcaggaaaaa caacattaac tgaatcactt ttatataact
      481 ctggtgcaat tactgaactt ggttcagtag ataaaggtac tactcgtact gataatacat
      541 tattagaacg tcaacgtgga atcacaattc aaacaggtat cacatctttt caatgggaaa
      601 atacaaaagt aaatattata gatacacctg gacacatgga tttccttgca gaagtatacc
      661 gtagtctttc agtattagat ggtgctattt tacttatcag cgctaaagat ggagttcaag
      721 ctcaaactcg tatcttattt cacgcattac gtaaaatggg tattccaaca attttcttta
      781 taaacaaaat tgaccaaaac ggaattgatt taagtacagt ttatcaagat atcaaagaaa
      841 aactttctgc tgaaatcgtt attaaacaaa aagttgaatt atacccaaac gtttgcgtaa
      901 caaattttac tgaatcagaa caatgggata cagttataga aggtaatgat gatttattag
      961 aaaaatacat gtcaggtaaa tcattagaag cattagaatt agaacaagaa gaaagtattc
     1021 gtttccaaaa ctgttcttta ttccctttat accatggaag cgctaaaagt aacataggta
     1081 ttgataactt aattgaagtt attactaaca aattttactc ttcaactcat cgtggtcctt
     1141 ctgaattatg cggtaacgtt ttcaaaattg aatatacaaa aaaacgtcaa cgtttagctt
     1201 atatacgtct ttatagtggt gttttacatt tacgtgatag tgttcgtgtt agtgaaaaag
     1261 aaaagattaa agttacagaa atgtatactt ctattaacgg tgaattatgc aaaattgacc
     1321 gtgcatattc aggtgaaatt gtaattttac aaaacgaatt tcttaaactt aatagtgtac
     1381 ttggtgacac aaaactttta ccacaacgta agaaaattga aaatccacac ccattacttc
     1441 aaacaacagt agaaccaagc aaacctgaac aacgtgaaat gcttttagat gctcttttag
     1501 aaattagtga ctctgaccca cttttacgtt actatgtaga ttctactact catgaaatta
     1561 ttctttcttt ccttggtaaa gttcaaatgg aagttatttc tgcattatta caagaaaaat
     1621 atcatgttga aatcgaatta aaagaaccta ctgtaattta tatggaacgt ccattaaaaa
     1681 atgctgaata tacaattcat attgaagttc caccaaatcc attttgggct tctattggtc
     1741 tttctgtttc tccacttcca cttggtagcg gaatgcaata tgaaagtagc gtaagtttag
     1801 gttatcttaa tcaaagtttc caaaacgcag ttatggaagg tattcgttac ggttgcgaac
     1861 aaggtttata cggttggaat gttacagact gcaaaatctg ttttaagtat ggactttact
     1921 attcacctgt atcaacacct gctgactttc gtatgcttgc accaattgtt ttagaacaag
     1981 ttttaaagaa agctggaact gaacttttag aaccatacct ttcttttaaa atctatgcac
     2041 cacaagaata cttaagtcgt gcttataacg atgcacctaa atactgtgct aatattgttg
     2101 atactcaatt aaagaacaac gaagtaattt taagcggaga aattcctgca cgttgtattc
     2161 aagaatatcg tagtgattta acatttttca ctaatggacg ttctgtttgc ttaactgaat
     2221 taaaaggtta tcatgttact actggtgaac ctgtatgcca accacgtcgt cctaatagtc
     2281 gtattgataa agttcgttat atgttcaaca aaatcacata ataatactag tagctcataa
     2341 aaaatttatt tgctttcagg aaattttttc tgtrtaatag attcatacga ctcactatat
     2401 ggaggaaatc tagatggaaa aaaagataac tgggtacaca actgttgata tttcacaatg
     2461 gcatagaaaa gagcactttg aggcctttca atcagttgct caatgcactt acaatcaaac
     2521 agttcaatta gacataacag catttctaaa aacagttaaa aagaacaaac acaagttcta
     2581 cccagcattt attcatatat tagcaagact tatgaacgct cacccagaat ttcgtatggc
     2641 tatgaaagac ggtgagcttg ttatctggga ctcagttcat ccttgttata cagtatttca
     2701 tgaacagact gagacattca gtagcttgtg gtcagaatat catgacgatt tcagacaatt
     2761 tcttcacatc tatagtcaag atgttgcatg ttacggtgaa aatttggcat acttccctaa
     2821 gggatttatt gagaacatgt tcttcgtgtc tgctaatcca tgggttagct ttactagctt
     2881 cgatttaaat gttgcaaaca tggacaactt ctttgctcct gtattcacta tgggaaagta
     2941 ttatacacag ggtgataaag wattaatgcc attggcaatc caggtacatc acgctgtatg
     3001 cgacggattc catgtagggc gtatgttgaa tgaattacaa caatattgtg atgaatggca
     3061 aggtggagct taactcgagc tgcagtaata cgactcacta tagggcatac gatttaggtg
     3121 acactatagt agggataaca gggtaattta gtaggtaggg ctatatggtc gttatgcgat
     3181 actgatgcat aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3241 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3301 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3361 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3421 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3481 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3541 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3601 gcggtgctac agagttcttg aagttgtggc ctaactacgg ctacactaga agaacagtat
     3661 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3721 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3781 gcagaaaaaa aggatctcaa cgcgcaatta accctcacta aagggaacaa aagctggagc
     3841 gctgcaggaa ttcgatatca agcttatcga taccgtcgac gccggccagc ctcgcagagc
     3901 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     3961 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     4021 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaagcatg gatataccga
     4081 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgct ggtacccagc
     4141 gatgaggatg aacccactca gggacctgat gtgacgtgcc gtgaacacga gcgtgtttag
     4201 tttttgatgg ttgtcaagtt cttttcattg tattcaaacc cccttttaaa gttgataatt
     4261 cttgttaatt ataatataag tgctggaaaa agtatagttt tttattgtta tttttaagaa
     4321 aatttatcca ttgtgttttt atatgaactt tttcaacatt ttttataacc ttttgtggaa
     4381 aagcggaaaa aagatatata aatactaaac tattgctttt tagtgttatt cattatgttt
     4441 atttcttttt aaaatatttt tttatccaca cattgtggaa gacttttatt attatgtgtt
     4501 ttaatttatt ttatgaaggg gaattaatat ggaaacaaaa gcgctttgag aaaaattaat
     4561 taataagtta aagaaggaaa aattaataga ccaagacatt attgaagaac atattgttac
     4621 ttcagaatta attaaaattt caaatacaga gttcgtaatc cttgttagaa gtaatcttgg
     4681 tgtaactatt ttaaatgaat ttaaagaagt atttgtgtac gaatttaaat aagttttaaa
     4741 cagttatgtg tctgttgatt ttttaactaa agaaattttt gagaaaaata caaaaaaaga
     4801 gaataaaaaa gaacccataa atactgtttt atctgaaaat gcacttactt ttgagaattt
     4861 tatagttggg tcaagtaata aacaagctaa tttagctgct aaaaatgttg ttgcaaaccc
     4921 tggtatgagt tttaatcctt tatttattta tggagattca ggactgggta aaacacattt
     4981 attacaagct attaaaaatc aagcagaatt aaatggaaaa aaagttttat atttaacatc
     5041 tgaagaattt acaaaaagaa ttgttaatgc tttaaacaaa ggtgatttaa gtgaaataga
     5101 agaattaaaa actgagataa attctaatga attctttatt ttagatgatg ttcaattttt
     5161 aagtaaaaaa gataaaacaa acgagttttt ctttaacatt attaacaatt ttactgaaaa
     5221 tggtaaacaa ttagtttttt ctagtgataa gactcctgaa ttgttaaatg gttttgataa
     5281 aagaatgata actagattta attcaggttt atcaacacct attaatgctt tagatattcc
     5341 aactgccaaa ttgataattg aagctgaaat taaaaaacaa ggtttaaaac aaaaaattaa
     5401 agaagatgcc gttgtttact tagctcaaaa ctttagcgat gatgttagaa aaattaaagg
     5461 tttagttaat agattacttt tctttggtat tcaaaacgat ttaggtcaca taattgattt
     5521 ggaagatgtt attgacttat ttaaagatac accttcagct aatttaggat tattaaatgt
     5581 taaaaaaatt aaagaagttg ttgctaaaaa atatgatgtt actataaaag ccatcgatgg
     5641 taaagcaaga acaactgcta taaaaaatgc tagacatctt tctatgtatt ttgcaaaaat
     5701 aattttaaac catacatcaa ctcaaattgg tgcagaattt ggtggaagag accatagtac
     5761 agttttaagt gctatttcgc gtattgaaaa attaatatat aaagaaaaag aattcaaaaa
     5821 aatagttgag tctttaaaaa acgaaataat aggaaaatag atgtaaattc gcatctattt
     5881 tttttgtgtg gaaaagaaat taaacttgtt gaataaaaag tcatttaaac ctttatttac
     5941 cgttgtttta tgagtgtttt ccacatatca actgcattat aaatattact tatataaaga
     6001 ataatataat atataaaagc atgcgtattt cttttgtttt tattttataa atatgagata
     6061 atatatattg taaataagag gtgttatatg ag
//
