LOCUS       sequence-19923-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Kraynov VS, Chamberlain C, Bokoch GM, Schwartz MA, Slabaugh S, Hahn 
            KM
  TITLE     Localized Rac activation dynamics visualized in living cells.
  JOURNAL   Science. 2000 Oct 13. 290(5490):333-7.
  PUBMED    11030651
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2000 Oct 13. 290(5490):333-7."
COMMENT     Sequence Label: pcDNA3-EGFP-Rac1(wt)
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..586
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     promoter        complement(614..632)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(615..632)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(652..669)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    658..769
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggaattcatg caggccatca agtgtgtggt ggtgggagac ggagctgtag gtaaaacttg
       61 cctactgatc agttacacaa ccaatgcatt tcctggagaa tatatcccta ctgtctttga
      121 caattattct gccaatgtta tggtagatgg aaaaccggtg aatctgggct tatgggatac
      181 agctggacaa gaagattatg acagattacg ccccctatcc tatccgcaaa cagatgtgtt
      241 cttaatttgc ttttcccttg tgagtcctgc atcatttgaa aatgtccgtg caaagtggta
      301 tcctgaggtg cggcaccact gtcccaacac tcccatcatc ctagtgggaa ctaaacttga
      361 tcttagggat gataaagaca cgatcgagaa actgaaggag aagaagctga ctcccatcac
      421 ctatccgcag ggtctagcca tggctaagga gattggtgct gtaaaatacc tggagtgctc
      481 ggcgctcaca cagcgaggcc tcaagacagt gtttgacgaa gcgatccgag cagtcctctg
      541 cccgcctccc gtgaagaaga ggaagagaaa atgcctgctg ttgtaactcg agcatgcatc
      601 tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
      661 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
      721 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctg
//
