LOCUS       sequence-199230-        6211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains: complete oriC region of M. florum L1 (oriC4 fragment), 
            colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of
            transfer of RP4 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Matteau D, Pepin ME, Baby V, Gauthier S, Arango Giraldo M, Knight 
            TF, Rodrigue S
  TITLE     Development of oriC-Based Plasmids for Mesoplasma florum.
  JOURNAL   Appl Environ Microbiol. 2017 Mar 17;83(7). pii: e03374-16. doi: 
            10.1128/AEM.03374-16. Print 2017 Apr 1.
  PUBMED    28115382
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2017-03-17"; volume: "83(7). pii: 
            e03374-16. doi"; pages: " 10.1128/AEM.03374-16. Print 2017 Apr 1"
COMMENT     Sequence Label: pMflST-o4
FEATURES             Location/Qualifiers
     source          1..6211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3205..3224
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3205..3223
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3231..3248
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     rep_origin      complement(3331..3919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3411..3430)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3925..3943
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     oriT            4001..4110
                     /note="incP origin of transfer"
ORIGIN
        1 tccctttagt gagggttaat gtcgtgactg ggaaaaccct ggcggaattc ggccagtcta
       61 catgtactct ttttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat
      121 tatttaagtg actatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac
      181 tgtcaatttg atagcgggaa caaataatta gatgtccttt tttaggaggg cttagttttt
      241 tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccagag aatatctgta
      301 tgctttgtat acctatggtt atgcataaaa atcccggtga taaaagtatt tatcactggg
      361 atttttatgc ccttttgggt ttttgaatgg aggaaatcta gatgaaaatc ataaatatcg
      421 gtgtattagc tcacgttgat gcaggaaaaa caacattaac tgaatcactt ttatataact
      481 ctggtgcaat tactgaactt ggttcagtag ataaaggtac tactcgtact gataatacat
      541 tattagaacg tcaacgtgga atcacaattc aaacaggtat cacatctttt caatgggaaa
      601 atacaaaagt aaatattata gatacacctg gacacatgga tttccttgca gaagtatacc
      661 gtagtctttc agtattagat ggtgctattt tacttatcag cgctaaagat ggagttcaag
      721 ctcaaactcg tatcttattt cacgcattac gtaaaatggg tattccaaca attttcttta
      781 taaacaaaat tgaccaaaac ggaattgatt taagtacagt ttatcaagat atcaaagaaa
      841 aactttctgc tgaaatcgtt attaaacaaa aagttgaatt atacccaaac gtttgcgtaa
      901 caaattttac tgaatcagaa caatgggata cagttataga aggtaatgat gatttattag
      961 aaaaatacat gtcaggtaaa tcattagaag cattagaatt agaacaagaa gaaagtattc
     1021 gtttccaaaa ctgttcttta ttccctttat accatggaag cgctaaaagt aacataggta
     1081 ttgataactt aattgaagtt attactaaca aattttactc ttcaactcat cgtggtcctt
     1141 ctgaattatg cggtaacgtt ttcaaaattg aatatacaaa aaaacgtcaa cgtttagctt
     1201 atatacgtct ttatagtggt gttttacatt tacgtgatag tgttcgtgtt agtgaaaaag
     1261 aaaagattaa agttacagaa atgtatactt ctattaacgg tgaattatgc aaaattgacc
     1321 gtgcatattc aggtgaaatt gtaattttac aaaacgaatt tcttaaactt aatagtgtac
     1381 ttggtgacac aaaactttta ccacaacgta agaaaattga aaatccacac ccattacttc
     1441 aaacaacagt agaaccaagc aaacctgaac aacgtgaaat gcttttagat gctcttttag
     1501 aaattagtga ctctgaccca cttttacgtt actatgtaga ttctactact catgaaatta
     1561 ttctttcttt ccttggtaaa gttcaaatgg aagttatttc tgcattatta caagaaaaat
     1621 atcatgttga aatcgaatta aaagaaccta ctgtaattta tatggaacgt ccattaaaaa
     1681 atgctgaata tacaattcat attgaagttc caccaaatcc attttgggct tctattggtc
     1741 tttctgtttc tccacttcca cttggtagcg gaatgcaata tgaaagtagc gtaagtttag
     1801 gttatcttaa tcaaagtttc caaaacgcag ttatggaagg tattcgttac ggttgcgaac
     1861 aaggtttata cggttggaat gttacagact gcaaaatctg ttttaagtat ggactttact
     1921 attcacctgt atcaacacct gctgactttc gtatgcttgc accaattgtt ttagaacaag
     1981 ttttaaagaa agctggaact gaacttttag aaccatacct ttcttttaaa atctatgcac
     2041 cacaagaata cttaagtcgt gcttataacg atgcacctaa atactgtgct aatattgttg
     2101 atactcaatt aaagaacaac gaagtaattt taagcggaga aattcctgca cgttgtattc
     2161 aagaatatcg tagtgattta acatttttca ctaatggacg ttctgtttgc ttaactgaat
     2221 taaaaggtta tcatgttact actggtgaac ctgtatgcca accacgtcgt cctaatagtc
     2281 gtattgataa agttcgttat atgttcaaca aaatcacata ataatactag tagctcataa
     2341 aaaatttatt tgctttcatt tttctgtata atagattcat acgactcact atatggagga
     2401 aatctagaat gagagaagct gttatagcag aagtttcaac acaattatca gaagtagttg
     2461 gtgtaataga aagacattta gaacctactt tactcgctgt tcatttatat ggtagtgcag
     2521 ttgatggtgg attaaaaccc cacagcgata ttgatttatt agttacagtt actgttagat
     2581 tagacgaaac aactagacgt gcattaatca acgatttatt akaaacatca gcgagtccag
     2641 gagaatcaga gatattaaga gcagtagagg taacaattgt tgttcatgat gatattattc
     2701 catggagata tccagctaaa agagaacttc aatttggaga atggcaaaga aacgatattc
     2761 tagctggaat atttgaacca gcaacaattg atattgactt agcaatatta cttacgaaag
     2821 ctagagaaca tagtgttgca ttagttggtc ctgctgctga ggaattattt gaccctgttc
     2881 cagagcaaga tttatttgaa gctttaaacg aaactttaac attatggaac tcaccaccag
     2941 actgggcagg tgatgaaaga aatgttgttt taactttaag tagaatctgg tatagtgctg
     3001 taacaggaaa aattgctcct aaagatgtag ctgctgactg ggccatggaa agattaccag
     3061 cacaatatca accagttatt ttagaagcaa gacaagcgta tttaggtcag gaggatagat
     3121 tagctagtag agctgaccaa ttagaagaat ttgttcatta tgtaaaagga gaaattacta
     3181 aagtagtagg taaataagct gcagtaatac gactcactat agggcatacg atttaggtga
     3241 cactatagta gggataacag ggtaatttag taggtagggc tatatggtcg ttatgcgata
     3301 ctgatgcata aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3361 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3421 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3481 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3541 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3601 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3661 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3721 cggtgctaca gagttcttga agttgtggcc taactacggc tacactagaa gaacagtatt
     3781 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3841 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3901 cagaaaaaaa ggatctcaac gcgcaattaa ccctcactaa agggaacaaa agctggagcg
     3961 ctgcaggaat tcgatatcaa gcttatcgat accgtcgacg ccggccagcc tcgcagagca
     4021 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc
     4081 gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag
     4141 tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaagcatgg atataccgaa
     4201 aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgctg gtacccagcg
     4261 atgaggatga acccactcag ggacctgatg tgacgtgccg tgaacacgag cgtgtttagt
     4321 ttttgatggt tgtcaagttc ttttcattgt attcaaaccc ccttttaaag ttgataattc
     4381 ttgttaatta taatataagt gctggaaaaa gtatagtttt ttattgttat ttttaagaaa
     4441 atttatccat tgtgttttta tatgaacttt ttcaacattt tttataacct tttgtggaaa
     4501 agcggaaaaa agatatataa atactaaact attgcttttt agtgttattc attatgttta
     4561 tttcttttta aaatattttt ttatccacac attgtggaag acttttatta ttatgtgttt
     4621 taatttattt tatgaagggg aattaatatg gaaacaaaag cgctttgaga aaaattaatt
     4681 aataagttaa agaaggaaaa attaatagac caagacatta ttgaagaaca tattgttact
     4741 tcagaattaa ttaaaatttc aaatacagag ttcgtaatcc ttgttagaag taatcttggt
     4801 gtaactattt taaatgaatt taaagaagta tttgtgtacg aatttaaata agttttaaac
     4861 agttatgtgt ctgttgattt tttaactaaa gaaatttttg agaaaaatac aaaaaaagag
     4921 aataaaaaag aacccataaa tactgtttta tctgaaaatg cacttacttt tgagaatttt
     4981 atagttgggt caagtaataa acaagctaat ttagctgcta aaaatgttgt tgcaaaccct
     5041 ggtatgagtt ttaatccttt atttatttat ggagattcag gactgggtaa aacacattta
     5101 ttacaagcta ttaaaaatca agcagaatta aatggaaaaa aagttttata tttaacatct
     5161 gaagaattta caaaaagaat tgttaatgct ttaaacaaag gtgatttaag tgaaatagaa
     5221 gaattaaaaa ctgagataaa ttctaatgaa ttctttattt tagatgatgt tcaattttta
     5281 agtaaaaaag ataaaacaaa cgagtttttc tttaacatta ttaacaattt tactgaaaat
     5341 ggtaaacaat tagttttttc tagtgataag actcctgaat tgttaaatgg ttttgataaa
     5401 agaatgataa ctagatttaa ttcaggttta tcaacaccta ttaatgcttt agatattcca
     5461 actgccaaat tgataattga agctgaaatt aaaaaacaag gtttaaaaca aaaaattaaa
     5521 gaagatgccg ttgtttactt agctcaaaac tttagcgatg atgttagaaa aattaaaggt
     5581 ttagttaata gattactttt ctttggtatt caaaacgatt taggtcacat aattgatttg
     5641 gaagatgtta ttgacttatt taaagataca ccttcagcta atttaggatt attaaatgtt
     5701 aaaaaaatta aagaagttgt tgctaaaaaa tatgatgtta ctataaaagc catcgatggt
     5761 aaagcaagaa caactgctat aaaaaatgct agacatcttt ctatgtattt tgcaaaaata
     5821 attttaaacc atacatcaac tcaaattggt gcagaatttg gtggaagaga ccatagtaca
     5881 gttttaagtg ctatttcgcg tattgaaaaa ttaatatata aagaaaaaga attcaaaaaa
     5941 atagttgagt ctttaaaaaa cgaaataata ggaaaataga tgtaaattcg catctatttt
     6001 ttttgtgtgg aaaagaaatt aaacttgttg aataaaaagt catttaaacc tttatttacc
     6061 gttgttttat gagtgttttc cacatatcaa ctgcattata aatattactt atataaagaa
     6121 taatataata tataaaagca tgcgtatttc ttttgttttt attttataaa tatgagataa
     6181 tatatattgt aaataagagg tgttatatga g
//
