LOCUS       sequence-199233-        7827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses His-tagged iPOLYMER component in E. coli for protein 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Nakamura H, Lee AA, Afshar AS, Watanabe S, Rho E, Razavi S, Suarez 
            A, Lin YC, Tanigawa M, Huang B, DeRose R, Bobb D, Hong W, Gabelli 
            SB, Goutsias J, Inoue T
  TITLE     Intracellular production of hydrogels and synthetic RNA granules by 
            multivalent molecular interactions.
  JOURNAL   Nat Mater. 2017 Nov 6. pii: nmat5006. doi: 10.1038/nmat5006.
  PUBMED    29115293
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2017 Nov 6. pii: nmat5006. doi: 10.1038/nmat5006."
COMMENT     Sequence Label: YFP-FKBPx5-His
FEATURES             Location/Qualifiers
     source          1..7827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     190..209
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     293..312
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        293..311
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    312..336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             351..373
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(380..382,383..385,386..388,389..1099)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MAVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI
                     KVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVL
                     LEFVTAAGITLGMDELYK"
     primer_bind     complement(428..449)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(689..708)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1036..1057
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1112..1432
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1538..1858
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1889..2209
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             2252..2572
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             2615..2935
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             2984..3001
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3054..3072)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3068..3115
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3152..3607
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3239..3258)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3449..3470
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3699..4514)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3699..4514)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4422..4441
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4506..4525
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4636..5224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5125..5144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5378..5395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5410..5549
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5475..5494)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5635..5657
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5651..5842)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6651..7733)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7695..7714
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7734..7811)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
       61 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
      121 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
      181 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
      241 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
      301 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      361 tttaagaagg agatatacca tggcagtgag caagggcgag gagctgttca ccggggtggt
      421 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      481 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      541 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc
      601 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      661 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      721 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      781 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      841 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
      901 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      961 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     1021 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1081 catggacgag ctgtacaaga gtgctggtgg tggagtgcag gtggaaacca tctccccagg
     1141 agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct
     1201 tgaagatgga aagaaatttg attcctcccg ggacagaaac aagcccttta agtttatgct
     1261 aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca
     1321 gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat
     1381 catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaactgg aaagtgctgg
     1441 tggtagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg gtcctcgagc
     1501 tagtgctggt ggtagtgctg gtggtagtgc tggtggtgga gtgcaggtgg aaaccatctc
     1561 cccaggagac gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg
     1621 gatgcttgaa gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt
     1681 tatgctaggc aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt
     1741 gggtcagaga gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc
     1801 aggcatcatc ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaaa
     1861 gcttagtgct ggtggtagtg ctggtggtgg agtgcaggtg gaaaccatct ccccaggaga
     1921 cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg cactacaccg ggatgcttga
     1981 agatggaaag aaatttgatt cctcccggga cagaaacaag ccctttaagt ttatgctagg
     2041 caagcaggag gtgatccgag gctgggaaga aggggttgcc cagatgagtg tgggtcagag
     2101 agccaaactg actatatctc cagattatgc ctatggtgcc actgggcacc caggcatcat
     2161 cccaccacat gccactctcg tcttcgatgt ggagcttcta aaactggaag aattcagtgc
     2221 tggtggtagt gctggtggta gtgctggtgg tggagtgcag gtggaaacca tctccccagg
     2281 agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct
     2341 tgaagatgga aagaaatttg attcctcccg ggacagaaac aagcccttta agtttatgct
     2401 aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca
     2461 gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat
     2521 catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaactgg aagtcgacag
     2581 tgctggtggt agtgctggtg gtagtgctgg tggtggagtg caggtggaaa ccatctcccc
     2641 aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat
     2701 gcttgaagat ggaaagaaat ttgattcctc ccgggacaga aacaagccct ttaagtttat
     2761 gctaggcaag caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg
     2821 tcagagagcc aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg
     2881 catcatccca ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaaccgcg
     2941 ggcccgggat ccaccggatc tagataacgc ggccgcactc gagcaccacc accaccacca
     3001 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     3061 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     3121 aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     3181 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3241 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3301 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3361 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3421 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3481 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     3541 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     3601 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3661 aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc
     3721 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     3781 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     3841 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     3901 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     3961 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     4021 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     4081 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     4141 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     4201 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     4261 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     4321 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     4381 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     4441 ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt
     4501 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     4561 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4621 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4681 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4741 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     4801 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4861 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4921 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4981 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5041 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5101 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5161 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5221 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5281 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5341 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5401 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa
     5461 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     5521 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     5581 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5641 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     5701 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     5761 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     5821 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     5881 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     5941 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     6001 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     6061 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     6121 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     6181 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     6241 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     6301 gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     6361 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
     6421 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     6481 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     6541 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
     6601 gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg
     6661 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6721 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
     6781 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
     6841 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
     6901 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg
     6961 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
     7021 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
     7081 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc
     7141 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
     7201 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
     7261 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
     7321 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
     7381 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
     7441 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
     7501 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
     7561 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
     7621 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
     7681 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
     7741 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
     7801 tcgatggtgt ccgggatctc gacgctc
//
