LOCUS       sequence-199239-       10237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-host-range Cas9/gRNA co-expression plasmid with gRNA 
            targetting ADE2 from Ogataea (para)polymorpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM, 
            Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG
  TITLE     Genome editing in Kluyveromyces and Ogataea yeasts using a 
            broad-host-range Cas9/gRNA co-expression plasmid.
  JOURNAL   FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 
            10.1093/femsyr/foy012.
  PUBMED    29438517
REFERENCE   2  (bases 1 to 10237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2018-05-1"; volume: "18(3). pii: 4847887. doi"; pages:
            " 10.1093/femsyr/foy012"
COMMENT     Sequence Label: pUDP013
FEATURES             Location/Qualifiers
     source          1..10237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            101..1678
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     CDS             449..1477
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             449..1477
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             2056..2079
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2083..2103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2104..6204
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6208..6228
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        7147..7813
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     7683..7706
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     ncRNA           7820..7856
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        7877..7952
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           7957..8024
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(8066..8084)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(join(8518..9309,9310..9378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9141..9160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9379..9483)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9586..10174
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10075..10094
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttcctgcgt tatcccctga ttctgtggat aaccgtgcgg actatatgtg aaggcatggc
       61 tatggcacgg cagacattcc gccagatcat caataggcac gacatggagg cccagaatac
      121 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
      181 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
      241 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
      301 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
      361 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
      421 catcacatcc gaacataaac aaggatccat gggtaaaaag cctgaactca ccgcgacgtc
      481 tgtcgagaag tttctgatcg aaaagttcga cagcgtgtcc gacctgatgc agctctcgga
      541 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
      601 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
      661 cgcgctcccg attccggaag tgcttgacat tggggaattt agcgagagcc tgacctattg
      721 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
      781 tgttctgcaa ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
      841 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
      901 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
      961 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     1021 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     1081 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     1141 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     1201 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     1261 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     1321 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     1381 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     1441 ccgacgcccc agcactcgtc cgagggcaaa ggaataactc gaggacaata aaaagattct
     1501 tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc
     1561 aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt
     1621 aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgga
     1681 gctctcgaga accctcacct ttcgagagga cgatgcccgt gtctaaatga ttcgaccagc
     1741 ctaagaatgt tcaacaatct ataatcagtc catagtcaac aagagccctc aaagttgata
     1801 tcgtttagtg atctccctcg agatgagcgg aggatggttg ctttttgggg agccctaagt
     1861 ggtacgtgcg gcgagcggga tgtttcccca ttaggcaaag gacgaccggg taacccctcg
     1921 agaaaaaaaa tttttcgccg ctaatctggt gttatataaa gctccccctg ctcctggaat
     1981 ttttttcctt gtcaactcac accggaaatc aaggcatttc attctgagta gttcttaaaa
     2041 acataatcaa caatggatta taaagatgac gatgacaaac ctccaaaaaa gaagagaaag
     2101 gtcgataaga aatactcaat aggcttagat atcggcacaa atagcgtcgg atgggcggtg
     2161 atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc
     2221 cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa
     2281 gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt
     2341 tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga
     2401 cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga
     2461 aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa
     2521 aaattggtag attctactta taaagcggat ttgcgcttaa tctatttggc cttagcgcat
     2581 atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat
     2641 gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct
     2701 attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga
     2761 cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat
     2821 ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa
     2881 gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg
     2941 caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt
     3001 ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca
     3061 atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga
     3121 caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca
     3181 ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta
     3241 gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc
     3301 aagcaacgga cctttgacaa cggctctatt acccatcaaa ttcacttggg tgagctgcat
     3361 gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt
     3421 gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt
     3481 cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa
     3541 gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa
     3601 aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt
     3661 tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt
     3721 tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc
     3781 gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt
     3841 tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt
     3901 attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt
     3961 ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct
     4021 cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga
     4081 cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta
     4141 gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat
     4201 agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta
     4261 catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact
     4321 gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt
     4381 attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt
     4441 atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct
     4501 gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga
     4561 gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatcac
     4621 attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct
     4681 gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa
     4741 aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta
     4801 acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa
     4861 ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat
     4921 actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct
     4981 aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat
     5041 taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa
     5101 tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa
     5161 atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct
     5221 aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc
     5281 cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt
     5341 gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta
     5401 cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt
     5461 gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct
     5521 tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt
     5581 aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac
     5641 tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa
     5701 tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta
     5761 caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt
     5821 cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag
     5881 cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt
     5941 attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa
     6001 ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct
     6061 cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa
     6121 gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt
     6181 gatttgagtc agctaggagg tgaccctcca aaaaagaaga gaaaggtctg agcggttttg
     6241 tataactaaa taatattgga aactaaatac gaatacccaa attttttatc taaattttgc
     6301 cgaaagatta aaatctgcag agatatccga aacaggtaaa tggatgtttc aatccctgta
     6361 gtcagtcagg aacccatatt atattacagt attagtcgcc gcttaggcac gcctttaatt
     6421 agcaaaatca aaccttaagt gcatatgccg tataagggaa actcaaagaa ctggcatcgc
     6481 aaaaatgaaa acatacgttg aaactacggc aaaggattgg tcagatcgct tcatacaggg
     6541 aaagttcggc atcaacatct ttggataata tcagaatgag aaagaacaga tacgcagtac
     6601 gttttttggt gagctctttg cacttcttta gttctttcca tcaatatcag ttttttaaac
     6661 ttttaggact aaaagtgatg tttaacttca aaatgtttaa aattttgttc ttcccgacgt
     6721 tcattaagaa tactaataca ctttaataat tattttaata ttttgttcta aataatgact
     6781 tttaattaaa aaagataaaa tataaaaaca tcataataac tcaccagagg ttaagaacaa
     6841 aaaaacaaat tagatatctg ctaatccaat atagttaaat caatctttcc ttggtataat
     6901 gggtatatta catatatttc aaggaccgac actcctacca aatatctaaa atttaccata
     6961 ttaacataac atgtatataa acgtcaaatc ataatcagca ctaacgtctc acggatcgta
     7021 tatgccgtag cgacaatcta agaactatgc gaggacacgc tagggccaag gcaaaaagat
     7081 tccttgatta cgtaagggag ttagaatcat tttgaataaa aaacacgctt tttcagttcg
     7141 agtttatcat tatcaatact gccatttcaa agaatacgta aataattaat agtagtgatt
     7201 ttcctaactt tatttagtca aaaaattagc cttttaattc tgctgtaacc cgtacatgcc
     7261 caaaataggg ggcgggttac acagaatata taacatcgta ggtgtctggg tgaacagttt
     7321 attcctggca tccactaaat ataatggagc ccgcttttta agctggcatc cagaaaaaaa
     7381 aagaatccca gcaccaaaat attgttttct tcaccaacca tcagttcata ggtccattct
     7441 cttagcgcaa ctacagagaa caggggcaca aacaggcaaa aaacgggcac aacctcaatg
     7501 gagtgatgca acctgcctgg agtaaatgat gacacaaggc aattgaccca cgcatgtatc
     7561 tatctcattt tcttacacct tctattacct tctgctctct ctgatttgga aaaagctgaa
     7621 aaaaaaggtt gaaaccagtt ccctgaaatt attcccctac ttgactaata agtatataaa
     7681 gacggtaggt attgattgta attctgtaaa tctatttctt aaacttctta aattctactt
     7741 ttatagttag tctttttttt agttttaaaa caccaagaac ttagtttcga ataaacacac
     7801 ataaacaaac aaattccagc tgatgagtcc gtgaggacga aacgagtaag ctcgtcctgg
     7861 aattgatctg cttggcgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
     7921 atcaacttga aaaagtggca ccgagtcggt gcttttggcc ggcatggtcc cagcctcctc
     7981 gctggcgccg gctgggcaac atgcttcggc atggcgaatg ggacacaggc cccttttcct
     8041 ttgtcgatat catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg
     8101 ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta
     8161 tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca
     8221 aacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacaag
     8281 cttacgtctc acggatcgta tatgccgtag cgacaatcta agaactatgc gaggacacgc
     8341 tagcagcggt attcacgtag acggataggt atagccagac atcagcagca tacttcggga
     8401 accgtaggcc accgagcggc cgcgattatc aaaaaggatc ttcacctaga tccttttaaa
     8461 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8521 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8581 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8641 tgctgcaatg ataccgcggg acccacgctc accggctcca gatttatcag caataaacca
     8701 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8761 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8821 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8881 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8941 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9001 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9061 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9121 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9181 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9241 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9301 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9361 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9421 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9481 gcgcacattt ccccgaaaag tgccacctgt catgaccaaa atcccttaac gtgagttttc
     9541 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     9601 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9661 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     9721 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9781 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9841 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9901 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9961 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    10021 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    10081 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    10141 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    10201 gttcctggcc ttttgctggc cttttgctca catgttc
//
