LOCUS       sequence-199246-        5513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for titrating synthesis of FtsZ with doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Sekar K, Rusconi R, Sauls JT, Fuhrer T, Noor E, Nguyen J, Fernandez 
            VI, Buffing MF, Berney M, Jun S, Stocker R, Sauer U
  TITLE     Synthesis and degradation of FtsZ quantitatively predict the first 
            cell division in starved bacteria.
  JOURNAL   Mol Syst Biol. 2018 Nov 5;14(11):e8623. doi: 10.15252/msb.20188623.
  PUBMED    30397005
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2018-11-5"; volume: "14(11):e8623. doi"; pages: " 
            10.15252/msb.20188623"
COMMENT     Sequence Label: pPLtetO-FtsZ
FEATURES             Location/Qualifiers
     source          1..5513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(59..127,128..919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(277..296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2071..2293
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     complement(2785..2802)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2955..3041
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    3203..3221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3228..3246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             4739..4747
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4755..5378
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4828..4847)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      5432..5479
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
       61 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      121 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      181 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      241 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      301 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      361 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      421 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      481 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      541 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      601 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      661 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
      781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
      901 actgattaag cattggtaac tgtcatacca agtttactca tatatacttt agattgattt
      961 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1021 caaaatccct taacgtggag ttttcgttcc actgagcgtc agaccccgaa cctcagatcc
     1081 ttccgtgttt agccagtatg ttctctagtg tggttcgttg tttttgcgtg agccatgaga
     1141 acgaaccatt gagatcatac ttactttgca tgtcactcaa aaattttgcc tcaaaactgg
     1201 tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg ttatgtaggt
     1261 aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc tggttgttct
     1321 caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc aacgtatcag
     1381 tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt aaatctttac
     1441 ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta ttttcaagca
     1501 ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga gttttctttt
     1561 gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt tcaaaagact
     1621 taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa ggcaatatct
     1681 cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt gtccactgga
     1741 aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc atcagctctc
     1801 tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc atctgagcgt
     1861 attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca atcgtggggt
     1921 tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag tcatagcgac
     1981 taatcgctag ttcatttgct ttgaaaacaa ctaatttaga catacatctc aattggtcta
     2041 ggtgatttta atcactatac caattgagat gggctagtca atgataatta ctagtccttt
     2101 tcctttgagt tgtgggtatc tgtaaattct gctagacctt tgctggaaaa cttgtaaatt
     2161 ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt tatattcaag
     2221 tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata gatcccagcc
     2281 ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc
     2341 tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa
     2401 gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt
     2461 ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg
     2521 cactacaggc gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc
     2581 gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat ctgacttttt
     2641 gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat tatcccgtga
     2701 caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa ctggctgttt
     2761 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
     2821 gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa
     2881 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg
     2941 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     3001 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     3061 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc
     3121 atcaaattaa gcagaaggcc atccgtcagg atggcctttt tgcgtttcta caaactctgc
     3181 tagcaagtaa ggccgactcg agtccctatc agtgatagag attgacatcc ctatcagtga
     3241 tagagatact gagcacatca gcaggacgca tgaccgaatt cattaaattt aactttaaga
     3301 aggagatata atatgtttga accaatggaa cttaccaatg acgcggtgat taaagtcatc
     3361 ggcgtcggcg gcggcggcgg taatgctgtt gaacacatgg tgcgcgagcg cattgaaggt
     3421 gttgaattct tcgcggtaaa taccgatgca caagcgctgc gtaaaacagc ggttggacag
     3481 acgattcaaa tcggtagcgg tatcaccaaa ggactgggcg ctggcgctaa tccagaagtt
     3541 ggccgcaatg cggctgatga ggatcgcgat gcattgcgtg cggcgctgga aggtgcagac
     3601 atggtcttta ttgctgcggg tatgggtggt ggtaccggta caggtgcagc accagtcgtc
     3661 gctgaagtgg caaaagattt gggtatcctg accgttgctg tcgtcactaa gcctttcaac
     3721 tttgaaggca agaagcgtat ggcattcgcg gagcagggga tcactgaact gtccaagcat
     3781 gtggactctc tgatcactat cccgaacgac aaactgctga aagttctggg ccgcggtatc
     3841 tccctgctgg atgcgtttgg cgcagcgaac gatgtactga aaggcgctgt gcaaggtatc
     3901 gctgaactga ttactcgtcc gggtttgatg aacgtggact ttgcagacgt acgcaccgta
     3961 atgtctgaga tgggctacgc aatgatgggt tctggcgtgg cgagcggtga agaccgtgcg
     4021 gaagaagctg ctgaaatggc tatctcttct ccgctgctgg aagatatcga cctgtctggc
     4081 gcgcgcggcg tgctggttaa catcacggcg ggcttcgacc tgcgtctgga tgagttcgaa
     4141 acggtaggta acaccatccg tgcatttgct tccgacaacg cgactgtggt tatcggtact
     4201 tctcttgacc cggatatgaa tgacgagctg cgcgtaaccg ttgttgcgac aggtatcggc
     4261 atggacaaac gtcctgaaat cactctggtg accaataagc aggttcagca gccagtgatg
     4321 gatcgctacc agcagcatgg gatggctccg ctgacccagg agcagaagcc ggttgctaaa
     4381 gtcgtgaatg acaatgcgcc gcaaactgcg aaagagccgg attatctgga tatcccagca
     4441 ttcctgcgta agcaagctga ttaacataac cctaatgagt gagctaactt acattaattg
     4501 cgttgcgcct taattaacgg cactcctcag caaatataat gaccctcttg ataacccaag
     4561 agggcatttt ttaatgccca tggcgtttac cacagctaac accacgtcgt ccctatctgc
     4621 tgccctaggt ctatgagtgg ttgctggata actttacggg catgcataag gctcgtaggc
     4681 tatattcagg gagaccacaa cggtttccct ctacaaataa ttttgtttaa ctttgaaata
     4741 aggaggtaat acaaatgtct cgtttagata aaagtaaagt gattaacagc gcattagagc
     4801 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg
     4861 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag
     4921 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc
     4981 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg
     5041 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc
     5101 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg
     5161 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag
     5221 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat
     5281 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg
     5341 gattagaaaa acaacttaaa tgtgaaagtg ggtcttaata agcgactaaa aaattgaatg
     5401 tagggaacca acatgccagt tcgagcaata actagcataa ccccttgggg cctctaaacg
     5461 ggtcttgagg ggttttttgc tgaaacctca ggcgagaagc acacggtcac act
//
