LOCUS       sequence-199257-       12127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Transfer Plasmid with mCherry-tagged mouse Trim21.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12127)
  AUTHORS   Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, 
            Schuh M
  TITLE     A Method for the Acute and Rapid Degradation of Endogenous Proteins.
  JOURNAL   Cell. 2017 Nov 13. pii: S0092-8674(17)31255-2. doi: 
            10.1016/j.cell.2017.10.033.
  PUBMED    29153837
REFERENCE   2  (bases 1 to 12127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Nov 13. pii: S0092-8674(17)31255-2. doi: 
            10.1016/j.cell.2017.10.033."
COMMENT     Sequence Label: pSMPP-mCherry-mTrim21
FEATURES             Location/Qualifiers
     source          1..12127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(25..43)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(26..43)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(333..352)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(566..585)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     685..707
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(745..763)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        831..935
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(936..1004,1005..1796)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1154..1173)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1967..2555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2456..2475
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2709..2726
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2796..2816)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2801..3130
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2981..3116
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4264..4329
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4459..4479
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4904..5038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4941..4960)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4995..5014
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5207..5840
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5887..6012
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6505..6738
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6923..6967
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7252..7369
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7522..7929
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      7968..7977
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7974..8681
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8119..8137)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8387..8406
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8718..10106
                     /codon_start=1
                     /product="mouse RING E3 ubiquitin ligase that can be used 
                     together with antibodies to degrade intracellular proteins 
                     (Clift et al., 2018)"
                     /label=TRIM21
                     /note="binds to the Fc domain of antibodies"
                     /translation="MSLEKMWEEVTCSICLDPMVEPMSIECGHCFCKECIFEVGKNGGS
                     SCPECRQQFLLRNLRPNRHIANMVENLKQIAQNTKKSTQETHCMKHGEKLHLFCEEDGQ
                     ALCWVCAQSGKHRDHTRVPIEEAAKVYQEKIHVALEKLRKGKELAEKMEMDLTMQRTDW
                     KRNIDTQKSRIHAEFALQNSLLAQEEQRQLQRLEKDQREYLRLLGKKEAELAEKNQALQ
                     ELISELERRIRGSELELLQEVRIILERSGSWNLDTLDIDAPDLTSTCPVPGRKKMLRTC
                     WVHITLDRNTANSWLIISKDRRQVRMGDTHQNVSDNKERFSNYPMVLGAQRFSSGKMYW
                     EVDVTQKEAWDLGVCRDSVQRKGQFSLSPENGFWTIWLWQDSYEAGTSPQTTLHIQVPP
                     CQIGIFVDYEAGVVSFYNITDHGSLIYTFSECVFAGPLRPFFNVGFNYSGGNAAPLKLC
                     PLKM"
     primer_bind     complement(10219..10239)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10637..10648)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10680..11179
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10715..10738)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11112..11131
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11200..11799
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11200..11219)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11696..11716
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             11894..12127
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
       61 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
      121 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
      181 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
      241 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
      301 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
      361 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
      421 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
      481 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
      541 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
      601 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      661 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
      721 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      781 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      841 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      901 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      961 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1021 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1081 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1141 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     1201 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1261 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1321 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1381 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1441 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1501 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1561 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1621 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1681 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1741 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1801 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1861 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1921 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1981 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2041 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2101 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2161 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2221 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2281 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2341 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2401 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2461 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2521 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2581 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2641 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2701 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2761 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     2821 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2881 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2941 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3001 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3061 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3121 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     3181 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     3241 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     3301 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     3361 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     3421 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
     3481 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     3541 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     3601 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     3661 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     3721 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     3781 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     3841 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     3901 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     3961 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     4021 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
     4081 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
     4141 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
     4201 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     4261 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     4321 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     4381 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     4441 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     4501 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     4561 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     4621 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
     4681 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     4741 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
     4801 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     4861 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     4921 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4981 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     5041 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     5101 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     5161 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     5221 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     5281 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     5341 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     5401 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     5461 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     5521 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     5581 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     5641 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5701 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5761 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5821 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     5881 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     5941 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     6001 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     6061 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     6121 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     6181 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     6241 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     6301 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc gnccgctgat
     6361 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     6421 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     6481 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     6541 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     6601 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     6661 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     6721 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     6781 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     6841 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     6901 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     6961 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     7021 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     7081 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     7141 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     7201 attagtgaac ggatctcgac ggtatcgcca aatggcagta ttcatccaca attttaaaag
     7261 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     7321 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     7381 cagggacagc agagatccag tttggatcga taagcttgat atcgaattcc tgcagccccg
     7441 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     7501 aggtttggca agctagctgc agtaacgcca ttttgcaagg catggaaaaa taccaaacca
     7561 agaatagaga agttcagatc aagggcgggt acatgaaaat agctaacgtt gggccaaaca
     7621 ggatatctgc ggtgagcagt ttcggccccg gcccggggcc aagaacagat ggtcaccgca
     7681 gtttcggccc cggcccgagg ccaagaacag atggtcccca gatatggccc aaccctcagc
     7741 agtttcttaa gacccatcag atgtttccag gctcccccaa ggacctgaaa tgaccctgcg
     7801 ccttatttga attaaccaat cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc
     7861 gagctctata aaagagctca caacccctca ctcggcgcgc cagtcctccg acagactgag
     7921 tcgcccgggg gggatccact gtgatcatca gacgcgtaca gcatatggcc accatggtga
     7981 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     8041 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     8101 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     8161 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     8221 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     8281 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     8341 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     8401 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     8461 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     8521 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     8581 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     8641 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtccggactc agatctcgag
     8701 ctcaagcttc gaattcaatg tctctggaaa agatgtggga ggaggtcacc tgttctatct
     8761 gcctggatcc catggtggag cctatgagta tcgaatgtgg ccattgcttt tgcaaggaat
     8821 gcatttttga agttgggaag aatgggggca gttcatgtcc cgagtgccgg caacagtttc
     8881 tgctccgaaa cctcaggccc aatagacata tagccaacat ggtggaaaac cttaaacaga
     8941 tagcccagaa taccaagaag agtacccagg aaacgcactg catgaagcat ggagagaagc
     9001 ttcacctatt ctgtgaggaa gatgggcagg ccctttgctg ggtgtgtgcc cagtctggga
     9061 aacaccggga ccacaccagg gtccctattg aagaggctgc taaggtatac caggagaaga
     9121 tccacgtggc tttagaaaaa ctgagaaagg ggaaagagtt ggccgagaag atggaaatgg
     9181 atctcacgat gcaaagaaca gactggaaga ggaacattga cacccagaag tcgaggattc
     9241 acgcagagtt cgcacttcag aatagcttgc tggctcagga ggagcagagg cagctgcaga
     9301 ggctggagaa ggatcaaagg gagtacctga gactcctggg gaagaaggag gctgagctgg
     9361 ctgagaagaa ccaggccctg caggagctga tctcagagct ggagaggagg attcgtggtt
     9421 cagagctgga gctactgcag gaggtgagga tcatcctgga aaggagtgga tcctggaacc
     9481 tggacacgtt agatattgac gccccagacc taacaagcac atgccctgtg ccagggcgga
     9541 agaagatgct gaggacgtgt tgggttcata ttactctgga tcgcaacacc gccaactcat
     9601 ggctcatcat ctcaaaggat cggagacaag tgaggatggg agacacccat cagaacgtgt
     9661 ctgacaataa ggagaggttt agtaattacc ccatggtgct aggtgcccag agattctcct
     9721 ctgggaagat gtactgggag gtagatgtga ctcaaaagga ggcctgggat ctgggggttt
     9781 gcagagattc tgttcagagg aaagggcagt tttcactcag tcccgagaat ggcttctgga
     9841 ccatttggtt atggcaagac agctatgagg ctggtaccag tcctcagacc accctccaca
     9901 ttcaagtacc tccatgccaa attgggatct ttgtggacta tgaggctggc gttgtctcct
     9961 tctacaacat aactgaccat ggctccctca tttacacctt ctcggagtgt gtttttgctg
    10021 gacctctgcg acctttcttc aatgttggtt tcaattatag tgggggaaat gcagcgcctc
    10081 taaagctctg tccactaaag atgtgagcgg ccgcgactct agagtcgacc tgcaggcatg
    10141 caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
    10201 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
    10261 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
    10321 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
    10381 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
    10441 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
    10501 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
    10561 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
    10621 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccatc aattctaccg
    10681 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
    10741 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
    10801 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
    10861 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
    10921 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
    10981 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
    11041 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
    11101 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
    11161 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
    11221 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
    11281 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
    11341 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
    11401 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
    11461 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
    11521 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
    11581 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
    11641 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
    11701 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
    11761 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac tcgagggaat taattcgagc
    11821 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
    11881 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
    11941 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    12001 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    12061 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    12121 tctagca
//
