LOCUS       sequence-199260-       10046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM, 
            Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG
  TITLE     Genome editing in Kluyveromyces and Ogataea yeasts using a 
            broad-host-range Cas9/gRNA co-expression plasmid.
  JOURNAL   FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 
            10.1093/femsyr/foy012.
  PUBMED    29438517
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2018-05-1"; volume: "18(3). pii: 4847887. doi"; pages:
            " 10.1093/femsyr/foy012"
COMMENT     Sequence Label: pUDP002
FEATURES             Location/Qualifiers
     source          1..10046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             290..1318
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             290..1318
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             1897..1920
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1924..1944
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1945..6045
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6049..6069
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        6988..7654
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     7524..7547
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     complement(7716..7734)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(join(8168..8959,8960..9028))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8791..8810
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9029..9133)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9236..9824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9725..9744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     gene            join(9988..10046,1..1519)
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
ORIGIN
        1 atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat ttgcatccat
       61 acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc agacctgcga
      121 gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag
      181 aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc cttttaaaat
      241 cttgctagga tacagttctc acatcacatc cgaacataaa caaggatcca tgggtaaaaa
      301 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtgtc
      361 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
      421 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
      481 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
      541 tagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
      601 gcctgaaacc gaactgcccg ctgttctgca accggtcgcg gaggccatgg atgcgatcgc
      661 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
      721 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
      781 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
      841 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
      901 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
      961 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     1021 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
     1081 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     1141 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     1201 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     1261 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaataact
     1321 cgaggacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat
     1381 tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat
     1441 catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg
     1501 aatgctggtc gctatactgg agctctcgag aaccctcacc tttcgagagg acgatgcccg
     1561 tgtctaaatg attcgaccag cctaagaatg ttcaacaatc tataatcagt ccatagtcaa
     1621 caagagccct caaagttgat atcgtttagt gatctccctc gagatgagcg gaggatggtt
     1681 gctttttggg gagccctaag tggtacgtgc ggcgagcggg atgtttcccc attaggcaaa
     1741 ggacgaccgg gtaacccctc gagaaaaaaa atttttcgcc gctaatctgg tgttatataa
     1801 agctccccct gctcctggaa tttttttcct tgtcaactca caccggaaat caaggcattt
     1861 cattctgagt agttcttaaa aacataatca acaatggatt ataaagatga cgatgacaaa
     1921 cctccaaaaa agaagagaaa ggtcgataag aaatactcaa taggcttaga tatcggcaca
     1981 aatagcgtcg gatgggcggt gatcactgat gaatataagg ttccgtctaa aaagttcaag
     2041 gttctgggaa atacagaccg ccacagtatc aaaaaaaatc ttataggggc tcttttattt
     2101 gacagtggag agacagcgga agcgactcgt ctcaaacgga cagctcgtag aaggtataca
     2161 cgtcggaaga atcgtatttg ttatctacag gagatttttt caaatgagat ggcgaaagta
     2221 gatgatagtt tctttcatcg acttgaagag tcttttttgg tggaagaaga caagaagcat
     2281 gaacgtcatc ctatttttgg aaatatagta gatgaagttg cttatcatga gaaatatcca
     2341 actatctatc atctgcgaaa aaaattggta gattctactt ataaagcgga tttgcgctta
     2401 atctatttgg ccttagcgca tatgattaag tttcgtggtc attttttgat tgagggagat
     2461 ttaaatcctg ataatagtga tgtggacaaa ctatttatcc agttggtaca aacctacaat
     2521 caattatttg aagaaaaccc tattaacgca agtggagtag atgctaaagc gattctttct
     2581 gcacgattga gtaaatcaag acgattagaa aatctcattg ctcagctccc cggtgagaag
     2641 aaaaatggct tatttgggaa tctcattgct ttgtcattgg gtttgacccc taattttaaa
     2701 tcaaattttg atttggcaga agatgctaaa ttacagcttt caaaagatac ttacgatgat
     2761 gatttagata atttattggc gcaaattgga gatcaatatg ctgatttgtt tttggcagct
     2821 aagaatttat cagatgctat tttactttca gatatcctaa gagtaaatac tgaaataact
     2881 aaggctcccc tatcagcttc aatgattaaa cgctacgatg aacatcatca agacttgact
     2941 cttttaaaag ctttagttcg acaacaactt ccagaaaagt ataaagaaat cttttttgat
     3001 caatcaaaaa acggatatgc aggttatatt gatgggggag ctagccaaga agaattttat
     3061 aaatttatca aaccaatttt agaaaaaatg gatggtactg aggaattatt ggtgaaacta
     3121 aatcgtgaag atttgctgcg caagcaacgg acctttgaca acggctctat tacccatcaa
     3181 attcacttgg gtgagctgca tgctattttg agaagacaag aagactttta tccattttta
     3241 aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc gaattcctta ttatgttggt
     3301 ccattggcgc gtggcaatag tcgttttgca tggatgactc ggaagtctga agaaacaatt
     3361 accccatgga attttgaaga agttgtcgat aaaggtgctt cagctcaatc atttattgaa
     3421 cgcatgacaa actttgataa aaatcttcca aatgaaaaag tactaccaaa acatagtttg
     3481 ctttatgagt attttacggt ttataacgaa ttgacaaagg tcaaatatgt tactgaagga
     3541 atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag ccattgttga tttactcttc
     3601 aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag attatttcaa aaaaatagaa
     3661 tgttttgata gtgttgaaat ttcaggagtt gaagatagat ttaatgcttc attaggtacc
     3721 taccatgatt tgctaaaaat tattaaagat aaagattttt tggataatga agaaaatgaa
     3781 gatatcttag aggatattgt tttaacattg accttatttg aagataggga gatgattgag
     3841 gaaagactta aaacatatgc tcacctcttt gatgataagg tgatgaaaca gcttaaacgt
     3901 cgccgttata ctggttgggg acgtttgtct cgaaaattga ttaatggtat tagggataag
     3961 caatctggca aaacaatatt agattttttg aaatcagatg gttttgccaa tcgcaatttt
     4021 atgcagctga tccatgatga tagtttgaca tttaaagaag acattcaaaa agcacaagtg
     4081 tctggacaag gcgatagttt acatgaacat attgcaaatt tagctggtag ccctgctatt
     4141 aaaaaaggta ttttacagac tgtaaaagtt gttgatgaat tggtcaaagt aatggggcgg
     4201 cataagccag aaaatatcgt tattgaaatg gcacgtgaaa atcagacaac tcaaaagggc
     4261 cagaaaaatt cgcgagagcg tatgaaacga atcgaagaag gtatcaaaga attaggaagt
     4321 cagattctta aagagcatcc tgttgaaaat actcaattgc aaaatgaaaa gctctatctc
     4381 tattatctcc aaaatggaag agacatgtat gtggaccaag aattagatat taatcgttta
     4441 agtgattatg atgtcgatca cattgttcca caaagtttcc ttaaagacga ttcaatagac
     4501 aataaggtct taacgcgttc tgataaaaat cgtggtaaat cggataacgt tccaagtgaa
     4561 gaagtagtca aaaagatgaa aaactattgg agacaacttc taaacgccaa gttaatcact
     4621 caacgtaagt ttgataattt aacgaaagct gaacgtggag gtttgagtga acttgataaa
     4681 gctggtttta tcaaacgcca attggttgaa actcgccaaa tcactaagca tgtggcacaa
     4741 attttggata gtcgcatgaa tactaaatac gatgaaaatg ataaacttat tcgagaggtt
     4801 aaagtgatta ccttaaaatc taaattagtt tctgacttcc gaaaagattt ccaattctat
     4861 aaagtacgtg agattaacaa ttaccatcat gcccatgatg cgtatctaaa tgccgtcgtt
     4921 ggaactgctt tgattaagaa atatccaaaa cttgaatcgg agtttgtcta tggtgattat
     4981 aaagtttatg atgttcgtaa aatgattgct aagtctgagc aagaaatagg caaagcaacc
     5041 gcaaaatatt tcttttactc taatatcatg aacttcttca aaacagaaat tacacttgca
     5101 aatggagaga ttcgcaaacg ccctctaatc gaaactaatg gggaaactgg agaaattgtc
     5161 tgggataaag ggcgagattt tgccacagtg cgcaaagtat tgtccatgcc ccaagtcaat
     5221 attgtcaaga aaacagaagt acagacaggc ggattctcca aggagtcaat tttaccaaaa
     5281 agaaattcgg acaagcttat tgctcgtaaa aaagactggg atccaaaaaa atatggtggt
     5341 tttgatagtc caacggtagc ttattcagtc ctagtggttg ctaaggtgga aaaagggaaa
     5401 tcgaagaagt taaaatccgt taaagagtta ctagggatca caattatgga aagaagttcc
     5461 tttgaaaaaa atccgattga ctttttagaa gctaaaggat ataaggaagt taaaaaagac
     5521 ttaatcatta aactacctaa atatagtctt tttgagttag aaaacggtcg taaacggatg
     5581 ctggctagtg ccggagaatt acaaaaagga aatgagctgg ctctgccaag caaatatgtg
     5641 aattttttat atttagctag tcattatgaa aagttgaagg gtagtccaga agataacgaa
     5701 caaaaacaat tgtttgtgga gcagcataag cattatttag atgagattat tgagcaaatc
     5761 agtgaatttt ctaagcgtgt tattttagca gatgccaatt tagataaagt tcttagtgca
     5821 tataacaaac atagagacaa accaatacgt gaacaagcag aaaatattat tcatttattt
     5881 acgttgacga atcttggagc tcccgctgct tttaaatatt ttgatacaac aattgatcgt
     5941 aaacgatata cgtctacaaa agaagtttta gatgccactc ttatccatca atccatcact
     6001 ggtctttatg aaacacgcat tgatttgagt cagctaggag gtgaccctcc aaaaaagaag
     6061 agaaaggtct gagcggtttt gtataactaa ataatattgg aaactaaata cgaataccca
     6121 aattttttat ctaaattttg ccgaaagatt aaaatctgca gagatatccg aaacaggtaa
     6181 atggatgttt caatccctgt agtcagtcag gaacccatat tatattacag tattagtcgc
     6241 cgcttaggca cgcctttaat tagcaaaatc aaaccttaag tgcatatgcc gtataaggga
     6301 aactcaaaga actggcatcg caaaaatgaa aacatacgtt gaaactacgg caaaggattg
     6361 gtcagatcgc ttcatacagg gaaagttcgg catcaacatc tttggataat atcagaatga
     6421 gaaagaacag atacgcagta cgttttttgg tgagctcttt gcacttcttt agttctttcc
     6481 atcaatatca gttttttaaa cttttaggac taaaagtgat gtttaacttc aaaatgttta
     6541 aaattttgtt cttcccgacg ttcattaaga atactaatac actttaataa ttattttaat
     6601 attttgttct aaataatgac ttttaattaa aaaagataaa atataaaaac atcataataa
     6661 ctcaccagag gttaagaaca aaaaaacaaa ttagatatct gctaatccaa tatagttaaa
     6721 tcaatctttc cttggtataa tgggtatatt acatatattt caaggaccga cactcctacc
     6781 aaatatctaa aatttaccat attaacataa catgtatata aacgtcaaat cataatcagc
     6841 actaacgtct cacggatcgt atatgccgta gcgacaatct aagaactatg cgaggacacg
     6901 ctagggccaa ggcaaaaaga ttccttgatt acgtaaggga gttagaatca ttttgaataa
     6961 aaaacacgct ttttcagttc gagtttatca ttatcaatac tgccatttca aagaatacgt
     7021 aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag ccttttaatt
     7081 ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat ataacatcgt
     7141 aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag cccgcttttt
     7201 aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc ttcaccaacc
     7261 atcagttcat aggtccattc tcttagcgca actacagaga acaggggcac aaacaggcaa
     7321 aaaacgggca caacctcaat ggagtgatgc aacctgcctg gagtaaatga tgacacaagg
     7381 caattgaccc acgcatgtat ctatctcatt ttcttacacc ttctattacc ttctgctctc
     7441 tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt tccctgaaat tattccccta
     7501 cttgactaat aagtatataa agacggtagg tattgattgt aattctgtaa atctatttct
     7561 taaacttctt aaattctact tttatagtta gtcttttttt tagttttaaa acaccaagaa
     7621 cttagtttcg aataaacaca cataaacaaa caaacgagac ctgcggaggt ctccacaggc
     7681 cccttttcct ttgtcgatat catgtaatta gttatgtcac gcttacattc acgccctccc
     7741 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
     7801 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     7861 tttctgtaca aacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
     7921 ttgggacaag cttacgtctc acggatcgta tatgccgtag cgacaatcta agaactatgc
     7981 gaggacacgc tagcagctgt attcacgtag acggataggt atagccagac atcagcagca
     8041 tacttcggga accgtaggcc accgagcggc cgcgattatc aaaaaggatc ttcacctaga
     8101 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8161 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8221 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8281 ctggccccag tgctgcaatg ataccgcggg acccacgctc accggctcca gatttatcag
     8341 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8401 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8461 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8521 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8581 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8641 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8701 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8761 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8821 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8881 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8941 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9001 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9061 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9121 taggggttcc gcgcacattt ccccgaaaag tgccacctgt catgaccaaa atcccttaac
     9181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9361 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9841 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9901 cccctgattc tgtggataac cgtgcggact atatgtgaag gcatggctat ggcacggcag
     9961 acattccgcc agatcatcaa taggcacgac atggaggccc agaataccct ccttgacagt
    10021 cttgacgtgc gcagctcagg ggcatg
//
