LOCUS       sequence-199262-       10237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-host-range Cas9/gRNA co-expression plasmid with gRNA 
            targetting ADE2 from Kluyveromyces marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM, 
            Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG
  TITLE     Genome editing in Kluyveromyces and Ogataea yeasts using a 
            broad-host-range Cas9/gRNA co-expression plasmid.
  JOURNAL   FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 
            10.1093/femsyr/foy012.
  PUBMED    29438517
REFERENCE   2  (bases 1 to 10237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2018-05-1"; volume: "18(3). pii: 4847887. doi"; pages:
            " 10.1093/femsyr/foy012"
COMMENT     Sequence Label: pUDP082
FEATURES             Location/Qualifiers
     source          1..10237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            11..1588
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     CDS             359..1387
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             359..1387
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             1966..1989
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1993..2013
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2014..6114
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6118..6138
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        7057..7723
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     7593..7616
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     ncRNA           7730..7766
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        7787..7862
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(7976..7994)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(join(8428..9219,9220..9288))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9051..9070
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9289..9393)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9496..10084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9985..10004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caataggcac gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc
       61 aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt
      121 tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca
      181 gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc
      241 cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc
      301 ttttaaaatc ttgctaggat acagttctca catcacatcc gaacataaac aaggatccat
      361 gggtaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
      421 cagcgtgtcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
      481 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
      541 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
      601 tggggaattt agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
      661 gcaagacctg cctgaaaccg aactgcccgc tgttctgcaa ccggtcgcgg aggccatgga
      721 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
      781 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
      841 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
      901 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
      961 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     1021 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     1081 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     1141 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     1201 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     1261 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     1321 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     1381 ggaataactc gaggacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt
     1441 tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg
     1501 cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa
     1561 tcgtatgtga atgctggtcg ctatactgga gctctcgaga accctcacct ttcgagagga
     1621 cgatgcccgt gtctaaatga ttcgaccagc ctaagaatgt tcaacaatct ataatcagtc
     1681 catagtcaac aagagccctc aaagttgata tcgtttagtg atctccctcg agatgagcgg
     1741 aggatggttg ctttttgggg agccctaagt ggtacgtgcg gcgagcggga tgtttcccca
     1801 ttaggcaaag gacgaccggg taacccctcg agaaaaaaaa tttttcgccg ctaatctggt
     1861 gttatataaa gctccccctg ctcctggaat ttttttcctt gtcaactcac accggaaatc
     1921 aaggcatttc attctgagta gttcttaaaa acataatcaa caatggatta taaagatgac
     1981 gatgacaaac ctccaaaaaa gaagagaaag gtcgataaga aatactcaat aggcttagat
     2041 atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa
     2101 aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct
     2161 cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga
     2221 aggtatacac gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg
     2281 gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac
     2341 aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag
     2401 aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactta taaagcggat
     2461 ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt
     2521 gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa
     2581 acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg
     2641 attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc
     2701 ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct
     2761 aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact
     2821 tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt
     2881 ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact
     2941 gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa
     3001 gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc
     3061 ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa
     3121 gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg
     3181 gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt
     3241 acccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat
     3301 ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat
     3361 tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa
     3421 gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca
     3481 tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa
     3541 catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt
     3601 actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat
     3661 ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa
     3721 aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca
     3781 ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa
     3841 gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag
     3901 atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag
     3961 cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt
     4021 agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat
     4081 cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa
     4141 gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc
     4201 cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta
     4261 atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact
     4321 caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa
     4381 ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag
     4441 ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt
     4501 aatcgtttaa gtgattatga tgtcgatcac attgttccac aaagtttcct taaagacgat
     4561 tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt
     4621 ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag
     4681 ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa
     4741 cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat
     4801 gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt
     4861 cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc
     4921 caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat
     4981 gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat
     5041 ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc
     5101 aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt
     5161 acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga
     5221 gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc
     5281 caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt
     5341 ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa
     5401 tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa
     5461 aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa
     5521 agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt
     5581 aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt
     5641 aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc
     5701 aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa
     5761 gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt
     5821 gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt
     5881 cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt
     5941 catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca
     6001 attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa
     6061 tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg tgaccctcca
     6121 aaaaagaaga gaaaggtctg agcggttttg tataactaaa taatattgga aactaaatac
     6181 gaatacccaa attttttatc taaattttgc cgaaagatta aaatctgcag agatatccga
     6241 aacaggtaaa tggatgtttc aatccctgta gtcagtcagg aacccatatt atattacagt
     6301 attagtcgcc gcttaggcac gcctttaatt agcaaaatca aaccttaagt gcatatgccg
     6361 tataagggaa actcaaagaa ctggcatcgc aaaaatgaaa acatacgttg aaactacggc
     6421 aaaggattgg tcagatcgct tcatacaggg aaagttcggc atcaacatct ttggataata
     6481 tcagaatgag aaagaacaga tacgcagtac gttttttggt gagctctttg cacttcttta
     6541 gttctttcca tcaatatcag ttttttaaac ttttaggact aaaagtgatg tttaacttca
     6601 aaatgtttaa aattttgttc ttcccgacgt tcattaagaa tactaataca ctttaataat
     6661 tattttaata ttttgttcta aataatgact tttaattaaa aaagataaaa tataaaaaca
     6721 tcataataac tcaccagagg ttaagaacaa aaaaacaaat tagatatctg ctaatccaat
     6781 atagttaaat caatctttcc ttggtataat gggtatatta catatatttc aaggaccgac
     6841 actcctacca aatatctaaa atttaccata ttaacataac atgtatataa acgtcaaatc
     6901 ataatcagca ctaacgtctc acggatcgta tatgccgtag cgacaatcta agaactatgc
     6961 gaggacacgc tagggccaag gcaaaaagat tccttgatta cgtaagggag ttagaatcat
     7021 tttgaataaa aaacacgctt tttcagttcg agtttatcat tatcaatact gccatttcaa
     7081 agaatacgta aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattagc
     7141 cttttaattc tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata
     7201 taacatcgta ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc
     7261 ccgcttttta agctggcatc cagaaaaaaa aagaatccca gcaccaaaat attgttttct
     7321 tcaccaacca tcagttcata ggtccattct cttagcgcaa ctacagagaa caggggcaca
     7381 aacaggcaaa aaacgggcac aacctcaatg gagtgatgca acctgcctgg agtaaatgat
     7441 gacacaaggc aattgaccca cgcatgtatc tatctcattt tcttacacct tctattacct
     7501 tctgctctct ctgatttgga aaaagctgaa aaaaaaggtt gaaaccagtt ccctgaaatt
     7561 attcccctac ttgactaata agtatataaa gacggtaggt attgattgta attctgtaaa
     7621 tctatttctt aaacttctta aattctactt ttatagttag tctttttttt agttttaaaa
     7681 caccaagaac ttagtttcga ataaacacac ataaacaaac aaagtcaagc tgatgagtcc
     7741 gtgaggacga aacgagtaag ctcgtcgccc atttttctgc gtataggttt tagagctaga
     7801 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     7861 gcttttggtc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac atgcttcggc
     7921 atggcgaatg ggacacaggc cccttttcct ttgtcgatat catgtaatta gttatgtcac
     7981 gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac
     8041 ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta
     8101 tatttcaaat ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac attatactga
     8161 aaaccttgct tgagaaggtt ttgggacaag cttacgtctc acggatcgta tatgccgtag
     8221 cgacaatcta agaactatgc gaggacacgc tagcagctgt attcacgtag acggataggt
     8281 atagccagac atcagcagca tacttcggga accgtaggcc accgagcggc cgcgattatc
     8341 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8401 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8461 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8521 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcggg acccacgctc
     8581 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8641 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8701 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8761 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8821 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8881 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8941 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9001 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9061 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9121 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9181 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9241 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9301 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9361 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgt
     9421 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9481 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9541 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9601 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9661 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9721 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9781 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9841 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9901 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9961 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10021 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10081 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10141 catgttcttt cctgcgttat cccctgattc tgtggataac cgtgcggact atatgtgaag
    10201 gcatggctat ggcacggcag acattccgcc agatcat
//
