LOCUS       sequence-199263-       10237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-host-range Cas9/gRNA co-expression plasmid with gRNA 
            targetting ADE2 from Kluyveromyces lactis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM, 
            Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG
  TITLE     Genome editing in Kluyveromyces and Ogataea yeasts using a 
            broad-host-range Cas9/gRNA co-expression plasmid.
  JOURNAL   FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 
            10.1093/femsyr/foy012.
  PUBMED    29438517
REFERENCE   2  (bases 1 to 10237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2018-05-1"; volume: "18(3). pii: 4847887. doi"; pages:
            " 10.1093/femsyr/foy012"
COMMENT     Sequence Label: pUDP025
FEATURES             Location/Qualifiers
     source          1..10237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            20..1597
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     CDS             368..1396
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             368..1396
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             1975..1998
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2002..2022
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2023..6123
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6127..6147
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        7066..7732
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     7602..7625
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     ncRNA           7739..7775
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        7796..7871
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           7876..7943
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(7985..8003)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(join(8437..9228,9229..9297))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9060..9079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9298..9402)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9505..10093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9994..10013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccagatcatc aataggcacg acatggaggc ccagaatacc ctccttgaca gtcttgacgt
       61 gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt
      121 ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc
      181 ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca
      241 cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca
      301 tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca
      361 aggatccatg ggtaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
      421 aaagttcgac agcgtgtccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
      481 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
      541 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
      601 gcttgacatt ggggaattta gcgagagcct gacctattgc atctcccgcc gtgcacaggg
      661 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcaac cggtcgcgga
      721 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
      781 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
      841 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
      901 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
      961 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     1021 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     1081 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     1141 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     1201 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     1261 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     1321 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     1381 gagggcaaag gaataactcg aggacaataa aaagattctt gttttcaaga acttgtcatt
     1441 tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat
     1501 tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc
     1561 atgcgtcaat cgtatgtgaa tgctggtcgc tatactggag ctctcgagaa ccctcacctt
     1621 tcgagaggac gatgcccgtg tctaaatgat tcgaccagcc taagaatgtt caacaatcta
     1681 taatcagtcc atagtcaaca agagccctca aagttgatat cgtttagtga tctccctcga
     1741 gatgagcgga ggatggttgc tttttgggga gccctaagtg gtacgtgcgg cgagcgggat
     1801 gtttccccat taggcaaagg acgaccgggt aacccctcga gaaaaaaaat ttttcgccgc
     1861 taatctggtg ttatataaag ctccccctgc tcctggaatt tttttccttg tcaactcaca
     1921 ccggaaatca aggcatttca ttctgagtag ttcttaaaaa cataatcaac aatggattat
     1981 aaagatgacg atgacaaacc tccaaaaaag aagagaaagg tcgataagaa atactcaata
     2041 ggcttagata tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt
     2101 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
     2161 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
     2221 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
     2281 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
     2341 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
     2401 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctacttat
     2461 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
     2521 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
     2581 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
     2641 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
     2701 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
     2761 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca
     2821 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct
     2881 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga
     2941 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa
     3001 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat
     3061 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct
     3121 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag
     3181 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac
     3241 ggctctatta cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa
     3301 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga
     3361 attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg
     3421 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca
     3481 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta
     3541 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc
     3601 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc
     3661 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat
     3721 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt
     3781 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg
     3841 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa
     3901 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg
     3961 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt
     4021 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt
     4081 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac
     4141 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta
     4201 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg
     4261 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat
     4321 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt
     4381 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa
     4441 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa
     4501 ttagatatta atcgtttaag tgattatgat gtcgatcaca ttgttccaca aagtttcctt
     4561 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg
     4621 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta
     4681 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt
     4741 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc
     4801 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat
     4861 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga
     4921 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg
     4981 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag
     5041 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa
     5101 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa
     5161 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg
     5221 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg
     5281 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag
     5341 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat
     5401 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct
     5461 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca
     5521 attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat
     5581 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa
     5641 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct
     5701 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt
     5761 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat
     5821 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta
     5881 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa
     5941 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt
     6001 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt
     6061 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt
     6121 gaccctccaa aaaagaagag aaaggtctga gcggttttgt ataactaaat aatattggaa
     6181 actaaatacg aatacccaaa ttttttatct aaattttgcc gaaagattaa aatctgcaga
     6241 gatatccgaa acaggtaaat ggatgtttca atccctgtag tcagtcagga acccatatta
     6301 tattacagta ttagtcgccg cttaggcacg cctttaatta gcaaaatcaa accttaagtg
     6361 catatgccgt ataagggaaa ctcaaagaac tggcatcgca aaaatgaaaa catacgttga
     6421 aactacggca aaggattggt cagatcgctt catacaggga aagttcggca tcaacatctt
     6481 tggataatat cagaatgaga aagaacagat acgcagtacg ttttttggtg agctctttgc
     6541 acttctttag ttctttccat caatatcagt tttttaaact tttaggacta aaagtgatgt
     6601 ttaacttcaa aatgtttaaa attttgttct tcccgacgtt cattaagaat actaatacac
     6661 tttaataatt attttaatat tttgttctaa ataatgactt ttaattaaaa aagataaaat
     6721 ataaaaacat cataataact caccagaggt taagaacaaa aaaacaaatt agatatctgc
     6781 taatccaata tagttaaatc aatctttcct tggtataatg ggtatattac atatatttca
     6841 aggaccgaca ctcctaccaa atatctaaaa tttaccatat taacataaca tgtatataaa
     6901 cgtcaaatca taatcagcac taacgtctca cggatcgtat atgccgtagc gacaatctaa
     6961 gaactatgcg aggacacgct agggccaagg caaaaagatt ccttgattac gtaagggagt
     7021 tagaatcatt ttgaataaaa aacacgcttt ttcagttcga gtttatcatt atcaatactg
     7081 ccatttcaaa gaatacgtaa ataattaata gtagtgattt tcctaacttt atttagtcaa
     7141 aaaattagcc ttttaattct gctgtaaccc gtacatgccc aaaatagggg gcgggttaca
     7201 cagaatatat aacatcgtag gtgtctgggt gaacagttta ttcctggcat ccactaaata
     7261 taatggagcc cgctttttaa gctggcatcc agaaaaaaaa agaatcccag caccaaaata
     7321 ttgttttctt caccaaccat cagttcatag gtccattctc ttagcgcaac tacagagaac
     7381 aggggcacaa acaggcaaaa aacgggcaca acctcaatgg agtgatgcaa cctgcctgga
     7441 gtaaatgatg acacaaggca attgacccac gcatgtatct atctcatttt cttacacctt
     7501 ctattacctt ctgctctctc tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc
     7561 cctgaaatta ttcccctact tgactaataa gtatataaag acggtaggta ttgattgtaa
     7621 ttctgtaaat ctatttctta aacttcttaa attctacttt tatagttagt ctttttttta
     7681 gttttaaaac accaagaact tagtttcgaa taaacacaca taaacaaaca aattgaaact
     7741 gatgagtccg tgaggacgaa acgagtaagc tcgtctttca atacctcaag tgcttgtttt
     7801 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
     7861 cgagtcggtg cttttggccg gcatggtccc agcctcctcg ctggcgccgg ctgggcaaca
     7921 tgcttcggca tggcgaatgg gacacaggcc ccttttcctt tgtcgatatc atgtaattag
     7981 ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag
     8041 ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa
     8101 cgttatttat atttcaaatt tttctttttt ttctgtacaa acgcgtgtac gcatgtaaca
     8161 ttatactgaa aaccttgctt gagaaggttt tgggacaagc ttacgtctca cggatcgtat
     8221 atgccgtagc gacaatctaa gaactatgcg aggacacgct agcagctgta ttcacgtaga
     8281 cggataggta tagccagaca tcagcagcat acttcgggaa ccgtaggcca ccgagcggcc
     8341 gcgattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8401 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8461 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8521 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcggga
     8581 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8641 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8701 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8761 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8821 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8881 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8941 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9001 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9061 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9121 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9181 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9241 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9301 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9361 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9421 gccacctgtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9481 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9541 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9601 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9661 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9721 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9781 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9841 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9901 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9961 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10021 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10081 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10141 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtgcggacta
    10201 tatgtgaagg catggctatg gcacggcaga cattccg
//
