LOCUS       sequence-199282-        7407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9-WT mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Kim S, Bae T, Hwang J, Kim JS
  TITLE     Rescue of high-specificity Cas9 variants using sgRNAs with matched 
            5' nucleotides.
  JOURNAL   Genome Biol. 2017 Nov 15;18(1):218. doi: 10.1186/s13059-017-1355-3.
  PUBMED    29141659
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2017-11-15"; volume: "18(1):218. doi"; pages: " 
            10.1186/s13059-017-1355-3"
COMMENT     Sequence Label: p3s-Cas9-HN
FEATURES             Location/Qualifiers
     source          1..7407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        157..536
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        537..740
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     691..711
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     785..804
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        785..803
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      817..826
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             829..855
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             829..855
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             865..885
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             913..5016
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             913..5013
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     promoter        complement(5047..5065)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5048..5065)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5085..5102)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5091..5315
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5403..5420)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5574..6162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5654..5673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6333..7124,7125..7193))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6956..6975
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7194..7298)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7373..7392)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
       61 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
      121 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      181 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      241 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      301 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      361 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      421 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      481 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      541 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      601 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      661 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      721 aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg
      781 aaattaatac gactcactat agggagaccc aagcttgcca ccatggtgta cccctacgac
      841 gtgcccgact acgccgaatt gcctccaaaa aagaagagaa aggtagggat ccgaattccc
      901 ggggaaaaac cggacaagaa gtacagcatc ggcctggaca tcggtaccaa cagcgtgggc
      961 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga agttcaaggt gctgggcaac
     1021 accgaccgcc acagcatcaa gaagaacctg atcggcgccc tgctgttcga cagcggcgag
     1081 accgccgagg ccacccgcct gaagcgcacc gcccgccgcc gctacacccg ccgcaagaac
     1141 cgcatctgct acctgcagga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
     1201 ttccaccgcc tggaggagag cttcctggtg gaggaggaca agaagcacga gcgccacccc
     1261 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
     1321 ctgcgcaaga agctggtgga cagcaccgac aaggccgacc tgcgcctgat ctacctggcc
     1381 ctggcccaca tgatcaagtt ccgcggccac ttcctgatcg agggcgacct gaaccccgac
     1441 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
     1501 gagaacccca tcaacgccag cggcgtggac gccaaggcca tcctgagcgc ccgcctgagc
     1561 aagagccgcc gcctggagaa cctgatcgcc cagctgcccg gcgagaagaa gaacggcctg
     1621 ttcggcaacc tgatcgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
     1681 ctggccgagg acgccaagct gcagctgagc aaggacacct acgacgacga cctggacaac
     1741 ctgctggccc agatcggcga ccagtacgcc gacctgttcc tggccgccaa gaacctgagc
     1801 gacgccatcc tgctgagcga catcctgcgc gtgaacaccg agatcaccaa ggcccccctg
     1861 agcgccagca tgatcaagcg ctacgacgag caccaccagg acctgaccct gctgaaggcc
     1921 ctggtgcgcc agcagctgcc cgagaagtac aaggagatct tcttcgacca gagcaagaac
     1981 ggctacgccg gctacatcga cggcggcgcc agccaggagg agttctacaa gttcatcaag
     2041 cccatcctgg agaagatgga cggcaccgag gagctgctgg tgaagctgaa ccgcgaggac
     2101 ctgctgcgca agcagcgcac cttcgacaac ggcagcatcc cccaccagat ccacctgggc
     2161 gagctgcacg ccatcctgcg ccgccaggag gacttctacc ccttcctgaa ggacaaccgc
     2221 gagaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc cctggcccgc
     2281 ggcaacagcc gcttcgcctg gatgacccgc aagagcgagg agaccatcac cccctggaac
     2341 ttcgaggagg tggtggacaa gggcgccagc gcccagagct tcatcgagcg catgaccaac
     2401 ttcgacaaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
     2461 ttcaccgtgt acaacgagct gaccaaggtg aagtacgtga ccgagggcat gcgcaagccc
     2521 gccttcctga gcggcgagca gaagaaggcc atcgtggacc tgctgttcaa gaccaaccgc
     2581 aaggtgaccg tgaagcagct gaaggaggac tacttcaaga agatcgagtg cttcgacagc
     2641 gtggagatca gcggcgtgga ggaccgcttc aacgccagcc tgggcaccta ccacgacctg
     2701 ctgaagatca tcaaggacaa ggacttcctg gacaacgagg agaacgagga catcctggag
     2761 gacatcgtgc tgaccctgac cctgttcgag gaccgcgaga tgatcgagga gcgcctgaag
     2821 acctacgccc acctgttcga cgacaaggtg atgaagcagc tgaagcgccg ccgctacacc
     2881 ggctggggcc gcctgagccg caagcttatc aacggcatcc gcgacaagca gagcggcaag
     2941 accatcctgg acttcctgaa gagcgacggc ttcgccaacc gcaacttcat gcagctgatc
     3001 cacgacgaca gcctgacctt caaggaggac atccagaagg cccaggtgag cggccagggc
     3061 gacagcctgc acgagcacat cgccaacctg gccggcagcc ccgccatcaa gaagggcatc
     3121 ctgcagaccg tgaaggtggt ggacgagctg gtgaaggtga tgggccgcca caagcccgag
     3181 aacatcgtga tcgagatggc ccgcgagaac cagaccaccc agaagggcca gaagaacagc
     3241 cgcgagcgca tgaagcgcat cgaggagggc atcaaggagc tgggcagcca gatcctgaag
     3301 gagcaccccg tggagaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
     3361 aacggccgcg acatgtacgt ggaccaggag ctggacatca accgcctgag cgactacgac
     3421 gtggaccaca tcgtgcccca gagcttcctg aaggacgaca gcatcgacaa caaggtgctg
     3481 acccgcagcg acaagaaccg cggcaagagc gacaacgtgc ccagcgagga ggtggtgaag
     3541 aagatgaaga actactggcg ccagctgctg aacgccaagc tgatcaccca gcgcaagttc
     3601 gacaacctga ccaaggccga gcgcggcggc ctgagcgagc tggacaaggc cggcttcatc
     3661 aagcgccagc tggtggagac ccgccagatc accaagcacg tggcccagat cctggacagc
     3721 cgcatgaaca ccaagtacga cgagaacgac aagctgatcc gcgaggtgaa ggtgatcacc
     3781 ctgaagagca agctggtgag cgacttccgc aaggacttcc agttctacaa ggtgcgcgag
     3841 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtggtggg caccgccctg
     3901 atcaagaagt accccaagct ggagagcgag ttcgtgtacg gcgactacaa ggtgtacgac
     3961 gtgcgcaaga tgatcgccaa gagcgagcag gagatcggca aggccaccgc caagtacttc
     4021 ttctacagca acatcatgaa cttcttcaag accgagatca ccctggccaa cggcgagatc
     4081 cgcaagcgcc ccctgatcga gaccaacggc gagaccggcg agatcgtgtg ggacaagggc
     4141 cgcgacttcg ccaccgtgcg caaggtgctg agcatgcccc aggtgaacat cgtgaagaag
     4201 accgaggtgc agaccggcgg cttcagcaag gagagcatcc tgcccaagcg caacagcgac
     4261 aagctgatcg cccgcaagaa ggactgggac cccaagaagt acggcggctt cgacagcccc
     4321 accgtggcct acagcgtgct ggtggtggcc aaggtggaga agggcaagag caagaagctg
     4381 aagagcgtga aggagctgct gggcatcacc atcatggagc gcagcagctt cgagaagaac
     4441 cccatcgact tcctggaggc caagggctac aaggaggtga agaaggacct gatcatcaag
     4501 ctgcccaagt acagcctgtt cgagctggag aacggccgca agcgcatgct ggccagcgcc
     4561 ggcgagctgc agaagggcaa cgagctggcc ctgcccagca agtacgtgaa cttcctgtac
     4621 ctggccagcc actacgagaa gctgaagggc agccccgagg acaacgagca gaagcagctg
     4681 ttcgtggagc agcacaagca ctacctggac gagatcatcg agcagatcag cgagttcagc
     4741 aagcgcgtga tcctggccga cgccaacctg gacaaggtgc tgagcgccta caacaagcac
     4801 cgcgacaagc ccatccgcga gcaggccgag aacatcatcc acctgttcac cctgaccaac
     4861 ctgggcgccc ccgccgcctt caagtacttc gacaccacca tcgaccgcaa gcgctacacc
     4921 agcaccaagg aggtgctgga cgccaccctg atccaccaga gcatcaccgg tctgtacgag
     4981 acccgcatcg acctgagcca gctgggcggc gactaataac tcgagcatgc atctagaggg
     5041 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc
     5101 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     5161 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     5221 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     5281 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     5341 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5401 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5461 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5521 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5581 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5641 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5701 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5761 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5821 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5881 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5941 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6001 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6061 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6241 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6301 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6361 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6421 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6481 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6541 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6601 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6661 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6721 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6781 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6841 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6901 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6961 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7021 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7081 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7141 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7201 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7261 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7321 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     7381 gctctgatgc cgcatagtta agccagt
//
