LOCUS       sequence-199289-       10497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the 
            TEF1 promoter in yeast. Both cassettes are integrated in HO locus. 
            Assembly and characterization of the auxin-inducible degradation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Papagiannakis A, Niebel B, Wit EC, Heinemann M
  TITLE     Autonomous Metabolic Oscillations Robustly Gate the Early and Late 
            Cell Cycle.
  JOURNAL   Mol Cell. 2017 Jan 19;65(2):285-295. doi: 
            10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15.
  PUBMED    27989441
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.11.018"; journalName: "Mol Cell"; date: 
            "2017-01-19- 19"; volume: "65"; issue: "2"; pages: "285-295"
COMMENT     Sequence Label: pG2J
FEATURES             Location/Qualifiers
     source          1..10497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    77..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     82..104
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     101..117
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     101..117
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1377..1778
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1797..3521
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             3549..3578
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3585..3614
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3621..3650
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3800..3965
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3855..3879)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(3982..4171)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(4219..4350)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             complement(join(4393..5103,5104..5106,5107..5109))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4435..4456)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4784..4803
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5043..5064
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(5127..5525)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     gene            5608..6964
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        5608..5951
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5952..6761
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6019..6038)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6767..6964
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7572..7591)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7573..7591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7598..7615)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7598..7614)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7607..7629)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7914..7933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8046..8067
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8221..8240)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8340..8362
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8400..8418)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8486..8590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8591..8659,8660..9451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8809..8828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9622..10210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10111..10130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10364..10381
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccgcggct
       61 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      121 gattacgcca ctagtccgag gcctcgagat ccgatatcgc cgtggcggcc gccagctgaa
      181 gcttaattat cctgggcacg agtgaaacaa agctaaaacc tttatttagc atggccattg
      241 aatgtaacaa ttatatatat cgcaagcaca aaaaatcaag gagagagaac taccactttg
      301 ttcatgtgta caatgttcat tatctccata agcaaaaaaa aaaaaataga aaacatatgc
      361 tataaggttg atattctcac gagtaagcgg cacttgctac ttattgacat tgcagatttt
      421 tggctacaga aatagtatat tagagattat aattgctaat caaatcaaaa tataaaatta
      481 gtaaaccaaa ccatttatac ccttccttag tagttatgga ttgtttttta atgatatttc
      541 tgcaaaccaa agaaagattg ttatccagat agaatttagt tttgatattc atttttttgt
      601 tgaagattga acgccatatc tgggcctcat aattcaaaag acggtgccat tatcggtagc
      661 gtttcgcatt gtactggatt tcagaaattt cacagttgat gaatcgaaaa gaatggtctc
      721 attgcaacac gtaaggttaa gatgtccctt tttaccatta taggcaataa atgaatcata
      781 aaacgaccgt atactggtga aatagtaggg agaacgagta cctgtagtaa aaagtataaa
      841 tcatagttaa tcgggcaatg tccctcgatc aaggagtatt gtgtcatgtt cgagacaaac
      901 gccaacattt ttgtttcttt tggacaaatg ttgtttgcat ttatgatccg ttatattttg
      961 atctaatgta gagttgcacg tagttcttac tggcaaagaa atcgatgcat accaaaaaag
     1021 aataaaggtg atatttgatc tttaccgttt agttccaacg taaaattgtg cctttggact
     1081 taaaatggcg gggtgtacaa tatggacttc ctcttttctg gcaaccaaac ccatacatcg
     1141 ggattcctat aataccttcg ttggtctccc taacatgtag gtggcggagg ggagatatac
     1201 aatagaacag ataccagaca agacataatg ggctaaacaa gactacacca attacactgc
     1261 ctcattgatg gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc
     1321 atatcgaagt ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat
     1381 gcaacttctt ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc
     1441 aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa
     1501 cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt
     1561 ccttcattca cgcacactac tctctaatga gcaacggtat acggccttcc ttccagttac
     1621 ttgaatttga aataaaaaaa agtttgctgt cttgctatca agtataaata gacctgcaat
     1681 tattaatctt ttgtttcctc gtcattgttc tcgttccctt tcttccttgt ttctttttct
     1741 gcacaatatt tcaagctata ccaagcatac aatcaactat ctcatataca actagaatga
     1801 cgtacttccc ggaggaggtg gtggagcaca tcttcagctt cctgccggcg cagcgcgacc
     1861 gcaacacggt ctcgctcgtc tgcaaggtgt ggtacgagat cgagaggctg agccgccgcg
     1921 gcgtcttcgt gggcaactgc tacgccgtgc gcgccggccg cgtcgccgcg cggttcccca
     1981 acgtgcgggc gctcacggtg aaggggaagc cccacttcgc cgacttcaac ctcgtgcccc
     2041 ccgactgggg cggctacgcg gggccgtgga tcgaggcggc cgcgagggga tgccacggcc
     2101 tggaggagct caggatgaag cggatggtgg tgtccgacga gagcctcgag ctgctggctc
     2161 gctcgttccc gcggttcagg gctcttgttc ttatcagctg cgaggggttc agcactgacg
     2221 ggctagccgc cgtcgcgagc cattgcaagc ttctgaggga gttggatttg caggaaaatg
     2281 aagtggagga tcgagggcct aggtggcttt cctgcttccc tgattcctgc acatcacttg
     2341 tctcattgaa ttttgcctgc atcaaagggg aggttaatgc tggttcactg gagagacttg
     2401 ttagcaggtc cccaaacctg cggagtttga ggctgaatcg atctgtatcg gtagatacac
     2461 ttgcaaagat actactgcgt acccctaact tggaggattt ggggacaggg aatttgacag
     2521 atgacttcca aactgagtcc tactttaagc ttaccagtgc tctggagaaa tgcaagatgt
     2581 tgaggagttt gtctggattc tgggatgctt ctcctgtttg cctgtcattt atctaccccc
     2641 tgtgtgctca actgacagga ttgaacttga gctatgcacc cacacttgat gcttctgacc
     2701 ttacaaaaat gattagccgc tgtgtgaagc tccaacgcct ttgggtactg gattgtatct
     2761 cggacaaagg cttgcaagtg gtggcctcca gttgcaaaga cttgcaagaa ctcagggtat
     2821 ttccatcaga tttctacgta gctggttatt ctgcagtgac agaggaggga cttgttgcag
     2881 tatccttggg ctgtccaaaa ctgaactcac tactgtactt ctgtcaccaa atgactaatg
     2941 ctgcactagt tactgtcgcc aagaactgtc caaatttcac acgattcaga ctttgtattc
     3001 ttgagccagg gaagcctgat gttgtgacaa gccaaccatt agatgaaggc tttggagcta
     3061 ttgttcgtga gtgcaaggga ttacaacgtt tgtcaatatc tggtcttctc acagacaaag
     3121 ttttcatgta tattgggaaa tatgcaaaac aacttgagat gctttctata gcatttgctg
     3181 gtgacagtga taagggtatg atgcatgtta tgaatggatg caagaattta aggaaactgg
     3241 agataagaga tagcccgttt ggtgatgctg cactcttggg gaattttgct aggtacgaga
     3301 caatgcgatc cctttggatg tcatcttgca atgtcacgtt aaaggggtgc caagtccttg
     3361 cgtcaaagat gccgatgctc aatgttgagg tcataaatga gcgggatggt agcaatgaaa
     3421 tggaggaaaa ccatggagat ctgcctaaag tggagaaatt atatgtgtac cgcacaactg
     3481 ctggggcgag ggatgatgca ccaaattttg ttaaaatcct agtcgactcc ggttctgctg
     3541 ctagtggtga acaaaagttg atttctgaag aagatttgaa cggtgaacaa aagctaatct
     3601 ccgaggaaga cttgaacggt gaacaaaaat taatctcaga agaagacttg aacggatcca
     3661 ctagctaaaa caattcttcg ccagaggttt ggtcaagtct ccaatcaagg ttgtcggctt
     3721 gtctaccttg ccagaaattt acgaaaagat ggaaaagggt caaatcgttg gtagatacgt
     3781 tgttgacact tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt
     3841 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt
     3901 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt
     3961 cgctcgtacg ctgagctgga tcttcgagcg tcccaaaacc ttctcaagca aggttttcag
     4021 tataatgtta catgcgtaca cgcgtctgta cagaaaaaaa agaaaaattt gaaatataaa
     4081 taacgttctt aatactaaca taactataaa aaaataaata gggacctaga cttcaggttg
     4141 tctaactcct tccttttcgg ttagagcgga tcttagctag ccgcggtacc aagcttactc
     4201 gagttacccg ggtgatacct tcacgaacgc cgccgcctcc gggccaccgc ttgatttttg
     4261 gcaggaaacc atcacgttct tccggtatga tctcaccggt ggccatccca caacttgtgc
     4321 cttggccgga ggtttggctg gatctttagg ggtacctgcc ccagcaccag ccccggcgcc
     4381 tgcaccgtcg accttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
     4441 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactgggt
     4501 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
     4561 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
     4621 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
     4681 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
     4741 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
     4801 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
     4861 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt
     4921 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
     4981 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
     5041 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
     5101 gctcaccatt ttaataacct aggaaactta gattagattg ctatgctttc tttctaatga
     5161 gcaagaagta aaaaaagttg taatagaaca agaaaaatga aactgaaact tgagaaattg
     5221 aagaccgttt attaacttaa atatcaatgg gaggtcatcg aaagagaaaa aaatcaaaaa
     5281 aaaaattttc aagaaaaaga aacgtgataa aaatttttat tgcctttttc gacgaagaaa
     5341 aagaaacgag gcggtctctt ttttcttttc caaaccttta gtacgggtaa ttaacgacac
     5401 cctagaggaa gaaagagggg aaatttagta tgctgtgctt gggtgttttg aagtggtacg
     5461 gcgatgcgcg gagtccgaga aaatctggaa gagtaaaaaa ggagtagaaa cattttgaag
     5521 ctatggtgtg gaattccagc tgcgggttaa ttaaggcgcg ccagatctgt ttagcttgcc
     5581 tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt
     5641 cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat
     5701 ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat
     5761 tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat
     5821 tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc
     5881 ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa
     5941 cataaacaac catgggtaag gaaaagactc acgtttcgag gccgcgatta aattccaaca
     6001 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     6061 caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     6121 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     6181 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     6241 ctgcgatccc cggcaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     6301 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     6361 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     6421 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     6481 ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt
     6541 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     6601 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     6661 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     6721 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagtactg acaataaaaa
     6781 gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt
     6841 ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc
     6901 gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat
     6961 actgctgtcg attcgatact aacgccgcca tccagtgtcg aaaacgagct cgaattaatt
     7021 cctgggggaa caacttcaca gaatgttttg tcatattgtc gaagtggtca caaaacaaga
     7081 gaagttccgc caattataaa aagggaaccc gtatatttca gcttcacgga tgatttccag
     7141 ggtgagagta ctgtatatgg gcttacgata gaaggccata aaaatttctt gcttggcaac
     7201 aaaatagaag tgaaatcatg tcgaggctgc tgtgtgggag aacagcataa aatatcacaa
     7261 aaaaagaatc taaaacactg tgttgcttgt cccagaaagg gaatcaagta tttttataaa
     7321 gattggagtg gtaaaaatcg agtatgtgct agatgctatg gaagatacaa attcagcggt
     7381 catcactgta taaattgcaa gtatgtacca gaagcacgtg aagtgaaaaa ggcaaaagac
     7441 aaaggcgaaa aattgggcat tacgcccgaa ggtttgccag ttaaaggacc agagtgtata
     7501 aaatgtggcg gaatcttaca gtggcctatg cggccgctct agctagaact agtggatcga
     7561 tccccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     7621 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     7681 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     7741 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     7801 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     7861 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     7921 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7981 gggtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     8041 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
     8101 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     8161 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     8221 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     8281 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     8341 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     8401 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     8461 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8521 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8581 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8641 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8701 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8761 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8821 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8881 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8941 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9001 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9061 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9121 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9181 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9241 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9301 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9361 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9421 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9481 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9541 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9601 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9661 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9721 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9781 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9841 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9901 aagacgatag ttaccggata aggcacagcg gtcgggctga acggggggtt cgtgcacaca
     9961 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10021 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10081 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10141 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10201 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10261 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    10321 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    10381 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    10441 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaat
//
