LOCUS       sequence-199291-        2636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rosa26 gene targeting vector to induce a Cre-dependent mosaic of 
            cells expressing different fluorescent and marker proteins localized
            to the membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, 
            Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, 
            Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera 
            S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R
  TITLE     Dual ifgMosaic: A Versatile Method for Multispectral and 
            Combinatorial Mosaic Gene-Function Analysis.
  JOURNAL   Cell. 2017 Aug 10;170(4):800-814.e18. doi: 
            10.1016/j.cell.2017.07.031.
  PUBMED    28802047
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "800-814.e18"
COMMENT     Sequence Label: iMb-Control-Mosaic (IR98.10)
FEATURES             Location/Qualifiers
     source          1..2636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     167..186
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        167..185
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    204..1286
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(1293..1358,1359..1396)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     enhancer        1410..1789
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1791..2066
                     /label=chicken beta-actin promoter
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
      181 atagggcgaa ttggagctcc accgcggcag gccctccgag cgtggtggag ccgttctgtg
      241 agacagccgg gtacgagtcg tgacgctgga aggggcaagc gggtggtggg caggaatgcg
      301 gtccgccctg cagcaaccgg agggggaggg agaagggagc ggaaaagtct ccaccggacg
      361 cggccatggc tcgggggggg gggggcagcg gaggagcgct tccggccgac gtctcgtcgc
      421 tgattggctt cttttcctcc cgccgtgtgt gaaaacacaa atggcgtgtt ttggttggcg
      481 taaggcgcct gtcagttaac ggcagccgga gtgcgcagcc gccggcagcc tcgctctgcc
      541 cactgggtgg ggcgggaggt aggtggggtg aggcgagctg gacgtgcggg cgcggtcggc
      601 ctctggcggg gcgggggagg ggagggaggg tcagcgaaag tagctcgcgc gcgagcggcc
      661 gcccaccctc cccttcctct gggggagtcg ttttacccgc cgccggccgg gcctcgtcgt
      721 ctgattggct ctcggggccc agaaaactgg cccttgccat tggctcgtgt tcgtgcaagt
      781 tgagtccatc cgccggccag cgggggcggc gaggaggcgc tcccaggttc cggccctccc
      841 ctcggccccg cgccgcagag tctggccgcg cgcccctgcg caacgtggca ggaagcgcgc
      901 gctgggggcg gggacgggca gtagggctga gcggctgcgg ggcgggtgca agcacgtttc
      961 cgacttgagt tgcctcaaga ggggcgtgct gagccagacc tccatcgcgc actccgggga
     1021 gtggagggaa ggagcgaggg ctcagttggg ctgttttgga ggcaggaagc acttgctctc
     1081 ccaaagtcgc tctgagttgt tatcagtaag ggagctgcag tggagtaggc ggggagaagg
     1141 ccgcaccctt ctccggaggg gggaggggag tgttgcaata cctttctggg agttctctgc
     1201 tgcctcctgg cttctgagga ccgccctggg cctgggagaa tcccttcccc ctcttccctc
     1261 gtgatctgca actccagtct ttctagcatc tgtagggcgc agtagtccag ggtttccttg
     1321 atgatgtcat acttatcctg tccctttttt ttccacagct cgcggttgag gacaaactct
     1381 tcgcggtctt tccagtggtt aattaagtcg acattgatta ttgactagtt attaatagta
     1441 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     1501 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     1561 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     1621 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     1681 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     1741 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
     1801 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
     1861 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg cgcgcgccag
     1921 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
     1981 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
     2041 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
     2101 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
     2161 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
     2221 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
     2281 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct
     2341 ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca
     2401 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg
     2461 ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt
     2521 cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
     2581 cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc gggggg
//
