LOCUS       sequence-199292-        8040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rosa26 gene targeting vector to induce a Cre-dependent mosaic of 
            cells expressing different fluorescent and marker proteins localized
            to the membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, 
            Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, 
            Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera 
            S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R
  TITLE     Dual ifgMosaic: A Versatile Method for Multispectral and 
            Combinatorial Mosaic Gene-Function Analysis.
  JOURNAL   Cell. 2017 Aug 10;170(4):800-814.e18. doi: 
            10.1016/j.cell.2017.07.031.
  PUBMED    28802047
REFERENCE   2  (bases 1 to 8040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "800-814.e18"
COMMENT     Sequence Label: iMb-Control-Mosaic (IR98.10)
FEATURES             Location/Qualifiers
     source          1..8040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    14..4354
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(4405..4421)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4405..4421)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(4454..4472)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4482..4499)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4482..4498)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4491..4513)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4640..5068
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4727..4746)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4937..4958
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5119..5223
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5224..5292,5293..6084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5442..5461)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6255..6843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6744..6763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6997..7014
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7131..7152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7167..7172,7173..7190,7191..7197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7205..7221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7210..7232
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7229..7245
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7229..7245
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7264..7284
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7266..7284
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     7314..7330
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        7358..7737
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7738..7941
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7892..7912
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        7986..8004
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gatatcgaat tcgctagaag atgggcggga gtcttctggg caggcttaaa ggctaacctg
       61 gtgtgtgggc gttgtcctgc aggggaattg aacaggtgta aaattggagg gacaagactt
      121 cccacagatt ttcggttttg tcgggaagtt ttttaatagg ggcaaataag gaaaatggga
      181 ggataggtag tcatctgggg ttttatgcag caaaactaca ggttattatt gcttgtgatc
      241 cgcctcggag tattttccat cgaggtagat taaagacatg ctcacccgag ttttatactc
      301 tcctgcttga gatccttact acagtatgaa attacagtgt cgcgagttag actatgtaag
      361 cagaatttta atcattttta aagagcccag tacttcatat ccatttctcc cgctccttct
      421 gcagccttat caaaaggtat tttagaacac tcattttagc cccattttca tttattatac
      481 tggcttatcc aacccctaga cagagcattg gcattttccc tttcctgatc ttagaagtct
      541 gatgactcat gaaaccagac agattagtta catacaccac aaatcgaggc tgtagctggg
      601 gcctcaacac tgcagttctt ttataactcc ttagtacact ttttgttgat cctttgcctt
      661 gatccttaat tttcagtgtc tatcacctct cccgtcaggt ggtgttccac atttgggcct
      721 attctcagtc cagggagttt tacaacaata gatgtattga gaatccaacc taaagcttaa
      781 ctttccactc ccatgaatgc ctctctcctt tttctccatt tataaactga gctattaacc
      841 attaatggtt tccaggtgga tgtctcctcc cccaatatta cctgatgtat cttacatatt
      901 gccaggctga tattttaaga cattaaaagg tatatttcat tattgagcca catggtattg
      961 attactgctt actaaaattt tgtcattgta cacatctgta aaaggtggtt ccttttggaa
     1021 tgcaaagttc aggtgtttgt tgtctttcct gacctaaggt cttgtgagct tgtatttttt
     1081 ctatttaagc agtgctttct cttggactgg cttgactcat ggcattctac acgttattgc
     1141 tggtctaaat gtgattttgc caagcttctt caggacctat aattttgctt gacttgtagc
     1201 caaacacaag taaaatgatt aagcaacaaa tgtatttgtg aagcttggtt tttaggttgt
     1261 tgtgttgtgt gtgcttgtgc tctataataa tactatccag gggctggaga ggtggctcgg
     1321 agttcaagag cacagactgc tcttccagaa gtcctgagtt caattcccag caaccacatg
     1381 gtggctcaca accatctgta atgggatctg atgccctctt ctggtgtgtc tgaagaccac
     1441 aagtgtattc acattaaata aataaatcct ccttcttctt cttttttttt tttttaaaga
     1501 gaatactgtc tccagtagaa tttactgaag taatgaaata ctttgtgttt gttccaatat
     1561 ggtagccaat aatcaaatta ctctttaagc actggaaatg ttaccaagga actaattttt
     1621 atttgaagtg taactgtgga cagaggagcc ataactgcag acttgtggga tacagaagac
     1681 caatgcagac tttaatgtct tttctcttac actaagcaat aaagaaataa aaattgaact
     1741 tctagtatcc tatttgttta aactgctagc tttacttaac ttttgtgctt catctataca
     1801 aagctgaaag ctaagtctgc agccattact aaacatgaaa gcaagtaatg ataattttgg
     1861 atttcaaaaa tgtagggcca gagtttagcc agccagtggt ggtgcttgcc tttatgcctt
     1921 taatcccagc actctggagg cagagacagg cagatctctg agtttgagcc cagcctggtc
     1981 tacacatcaa gttctatcta ggatagccag gaatacacac agaaaccctg ttggggaggg
     2041 gggctctgag atttcataaa attataattg aagcattccc taatgagcca ctatggatgt
     2101 ggctaaatcc gtctaccttt ctgatgagat ttgggtatta ttttttctgt ctctgctgtt
     2161 ggttgggtct tttgacactg tgggctttct ttaaagcctc cttcctgcca tgtggtctct
     2221 tgtttgctac taacttccca tggcttaaat ggcatggctt tttgccttct aagggcagct
     2281 gctgagattt gcagcctgat ttccagggtg gggttgggaa atctttcaaa cactaaaatt
     2341 gtcctttaat ttttttttta aaaaatgggt tatataataa acctcataaa atagttatga
     2401 ggagtgaggt ggactaatat taaatgagtc cctcccctat aaaagagcta ttaaggcttt
     2461 ttgtcttata cttaactttt tttttaaatg tggtatcttt agaaccaagg gtcttagagt
     2521 tttagtatac agaaactgtt gcatcgctta atcagatttt ctagtttcaa atccagagaa
     2581 tccaaattct tcacagccaa agtcaaatta agaatttctg acttttaatg ttaatttgct
     2641 tactgtgaat ataaaaatga tagcttttcc tgaggcaggg tctcactatg tatctctgcc
     2701 tgatctgcaa caagatatgt agactaaagt tctgcctgct tttgtctcct gaatactaag
     2761 gttaaaatgt agtaatactt ttggaacttg caggtcagat tcttttatag gggacacact
     2821 aagggagctt gggtgatagt tggtaaaatg tgtttcaagt gatgaaaact tgaattatta
     2881 tcaccgcaac ctacttttta aaaaaaaaag ccaggcctgt tagagcatgc ttaagggatc
     2941 cctaggactt gctgagcaca caagagtagt tacttggcag gctcctggtg agagcatatt
     3001 tcaaaaaaca aggcagacaa ccaagaaact acagttaagg ttacctgtct ttaaaccatc
     3061 tgcatataca cagggatatt aaaatattcc aaataatatt tcattcaagt tttcccccat
     3121 caaattggga catggatttc tccggtgaat aggcagagtt ggaaactaaa caaatgttgg
     3181 ttttgtgatt tgtgaaattg ttttcaagtg atagttaaag cccatgagat acagaacaaa
     3241 gctgctattt cgaggtctct tggtttatac tcagaagcac ttctttgggt ttccctgcac
     3301 tatcctgatc atgtgctagg cctaccttag gctgattgtt gttcaaataa acttaagttt
     3361 cctgtcaggt gatgtcatat gatttcatat atcaaggcaa aacatgttat atatgttaaa
     3421 catttgtact taatgtgaaa gttaggtctt tgtgggtttg atttttaatt ttcaaaacct
     3481 gagctaaata agtcattttt acatgtctta catttggtgg aattgtataa ttgtggtttg
     3541 caggcaagac tctctgacct agtaacccta cctatagagc actttgctgg gtcacaagtc
     3601 taggagtcaa gcatttcacc ttgaagttga gacgttttgt tagtgtatac tagtttatat
     3661 gttggaggac atgtttatcc agaagatatt caggactatt tttgactggg ctaaggaatt
     3721 gattctgatt agcactgtta gtgagcattg agtggccttt aggcttgaat tggagtcact
     3781 tgtatatctc aaataatgct ggcctttttt aaaaagccct tgttctttat caccctgttt
     3841 tctacataat ttttgttcaa agaaatactt gtttggatct ccttttgaca acaatagcat
     3901 gttttcaagc catatttttt ttcctttttt tttttttttt tggtttttcg agacagggtt
     3961 tctctgtata gccctggctg tcctggaact cactttgtag accaggctgg cctcgaactc
     4021 agaaatccgc ctgcctctgc ctcctgagtg ccgggattaa aggcgtgcac caccacgcct
     4081 ggctaagttg gatattttgt tatataacta taaccaatac taactccact gggtggattt
     4141 ttaattcagt cagtagtctt aagtggtctt tattggccct tcattaaaat ctactgttca
     4201 ctctaacaga ggctgttggt actagtggca cttaagcaac ttcctacgga tatactagca
     4261 gattaagggt cagggataga aactagtcta gcgttttgta tacctaccag ctttatacta
     4321 ccttgttctg atagaaatat ttcaggacat ctagcttatc gataccgtcg acgttcgaac
     4381 acgtggaatt cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg
     4441 agctccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     4501 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4561 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4621 agcctgaatg gcgaatggaa attgtactgt agcggcgcat taagcgcggc gggtgtggtg
     4681 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4741 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4801 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4861 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4921 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4981 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5041 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttgtggc
     5101 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5161 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5221 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5281 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5341 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5401 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5461 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5521 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5581 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5641 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5701 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5761 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5821 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5881 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5941 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6001 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6061 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6121 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6181 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6241 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6301 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6361 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6421 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6481 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6541 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6601 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6661 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6721 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6781 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6841 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6901 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6961 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7021 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     7081 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7141 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7201 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     7261 cgcgcaatta accctcacta aagggaacaa aagctgggta ccgggccccc cctcgaggtc
     7321 gacggtatcg ataagcttca cgtgttcgaa acgcgttgac attgattatt gactagttat
     7381 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     7441 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     7501 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     7561 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     7621 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     7681 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     7741 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     7801 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     7861 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     7921 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
     7981 gaaattaata cgactcacta tagggagacc caagctggct agcgtttaaa cttaagcttg
//
