LOCUS       sequence-199293-        8422 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rosa26 gene targeting vector to induce a Cre-dependent mosaic of 
            cells expressing different fluorescent and marker proteins localized
            to the membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, 
            Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, 
            Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera 
            S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R
  TITLE     Dual ifgMosaic: A Versatile Method for Multispectral and 
            Combinatorial Mosaic Gene-Function Analysis.
  JOURNAL   Cell. 2017 Aug 10;170(4):800-814.e18. doi: 
            10.1016/j.cell.2017.07.031.
  PUBMED    28802047
REFERENCE   2  (bases 1 to 8422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "800-814.e18"
COMMENT     Sequence Label: iMb-Control-Mosaic (IR98.10)
FEATURES             Location/Qualifiers
     source          1..8422
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     341..363
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     425..444
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(478..511)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(519..552)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(561..594)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      608..617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        668..1167
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(703..726)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1100..1119
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        1179..1226
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1245..2048
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1308..1327)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1918..1937
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2086..2310
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2467..2601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2504..2523)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2558..2577
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(2621..2654)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      2777..2786
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2783..2785,2786..2788,2789..3499)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2828..2849)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3089..3108)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3436..3457
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3509..3562
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3593..3970
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             3995..4048
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    4108..4332
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(4367..4400)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             4444..5871
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             5881..5934
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5965..6342
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             6367..6420
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    6468..6692
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(6727..6760)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6883..6892
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6889..7584
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             7603..7656
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7687..8064
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             8089..8142
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    8202..8409
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
       61 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
      121 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
      181 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
      241 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
      301 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
      361 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
      421 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cctcgagata
      481 acttcgtata gtatacctta tacgaagtta tcgagctcat aacttcgtat aggatacttt
      541 atacgaagtt ataactcgag ataacttcgt atagcataca ttatacgaag ttataccggc
      601 agatctcgcc accatggtga gtgtgattac agaggctagc ttggctgcag gtcgtcgaaa
      661 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
      721 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
      781 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
      841 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
      901 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
      961 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     1021 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     1081 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     1141 tcctcttcct catctccggg cctttcgacc tgcagcctgt tgacaattaa tcatcggcat
     1201 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggga tcggccattg
     1261 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     1321 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     1381 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     1441 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     1501 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     1561 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     1621 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     1681 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     1741 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg
     1801 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     1861 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     1921 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     1981 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     2041 tcttctgagg ggatcaattc tctagagctc gctgatcagc ctcgactgtg ccttctagtt
     2101 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     2161 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     2221 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     2281 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     2341 cgactagagc ttgcggaacc cttcgtaatc agccatacca catttgtaga ggttttactt
     2401 gctttaaaaa acctcccaca cctccccctg aacctgaaac atagaatgaa tgcaattgtt
     2461 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     2521 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     2581 gtatcttatc atgtctggat ctgacatggt aagtaagctt ataacttcgt atagtatacc
     2641 ttatacgaag ttatggcgcg cctaattcac agccaccatg ctgtgctgca tcagaagaac
     2701 taaaccggtt gagaagaatg aagaggccga tcaggagctg cagtcgacgg taccgcgggc
     2761 ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     2821 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2881 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2941 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc
     3001 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3061 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3121 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3181 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     3241 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3301 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3361 cgtgctgctg cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa
     3421 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3481 catggacgag ctgtacaagg gcagtggaga gggcagagga agtctgctaa catgcggtga
     3541 cgtcgaggag aatcctggcc cagaattctg tgtggatacc taccgctaca tcatgccaga
     3601 gccagcgaag tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca
     3661 gaagaaaggc ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtgta
     3721 caaggttctg aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat
     3781 gaactcgttt gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc gcctggcgca
     3841 ttacaacaag cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct
     3901 gcctggggag ttggccaagc acgccgtgtc cgagggcact aaggccatca ccaagtacac
     3961 cagcgctaag gatccaccgg tcgcctgaag tggagagggc agaggaagtc tgctaacatg
     4021 cggtgacgtc gaggagaatc ctggcccaga attctgcaga tatccagcac agtggcggcc
     4081 gctcgagtct aggcgcgcca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4141 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4201 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4261 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4321 tgggctctat ggcttctgag gcggaaagaa ccagctgggg agatctataa cttcgtatag
     4381 gatactttat acgaagttat ggtaccggcg cgccgatcca tgggttgctg tttctccaag
     4441 accatggtga gcaagggaga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg
     4501 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     4561 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
     4621 gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac
     4681 atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     4741 ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg
     4801 atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag
     4861 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag
     4921 ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag
     4981 accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag
     5041 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag
     5101 ggccgccacc acctgttcct ggggcatggc accggcagca ccggcagcgg cagctccggc
     5161 accgcctcct ccgaggacaa caacatggcc gtcatcaaag agttcatgcg cttcaaggtg
     5221 cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     5281 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
     5341 gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc
     5401 gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     5461 atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc
     5521 acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg
     5581 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
     5641 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccgcta cctggtggag
     5701 ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac
     5761 accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc
     5821 tccgagggcc gccaccacct gttcctgtac ggcatggacg agctgtacaa gggcagtgga
     5881 gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccagaattc
     5941 tgtgtggata cctaccgcta catcatgcca gagccagcga agtctgctcc cgccccgaaa
     6001 aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc
     6061 agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac
     6121 accggcattt cgtccaaggc catgggcatc atgaactcgt ttgtgaacga cattttcgag
     6181 cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc
     6241 agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg
     6301 tccgagggca ctaaggccat caccaagtac accagcgcta aggatccacc ggtcgccggc
     6361 agtggagagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca
     6421 gaattctgca gatatccagc acagtggcgg ccgctcgaga gcctcgactg tgccttctag
     6481 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6541 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6601 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6661 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     6721 actagtataa cttcgtatag catacattat acgaagttat ggcgcgccta attcacagcc
     6781 accatgctgt gctgcatcag aagaactaag ccggttgaga agaatgaaga ggccgatcag
     6841 gagctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcgag
     6901 ctgattaagg agaacatgca catgaagctc tacatggagg gcaccgtgaa caaccaccac
     6961 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     7021 aaggcggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
     7081 tacggcagca aaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc
     7141 ttccccgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
     7201 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     7261 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc
     7321 accgagaccc tgtaccccgc tgacggcggc ctggaaggca gagccgacat ggccctgaag
     7381 ctcgtgggcg ggggccacct gatctgcaac ttgaagacca catacagatc caagaaaccc
     7441 gctaagaacc tcaagatgcc cggcgtctac tatgtggaca gaagactgga aagaatcaag
     7501 gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
     7561 ctccctagca aactggggca cagaatgtac aagggcagtg gagagggcag aggaagtctg
     7621 ctaacatgcg gtgacgtcga ggagaatcct ggcccagaat tctgtgtgga tacctaccgc
     7681 tacatcatgc cagagccagc gaagtctgct cccgccccga aaaagggctc caagaaggcg
     7741 gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa ggagagctat
     7801 tccatctatg tgtacaaggt tctgaagcag gtccaccctg acaccggcat ttcgtccaag
     7861 gccatgggca tcatgaactc gtttgtgaac gacattttcg agcgcatcgc aggtgaggct
     7921 tcccgcctgg cgcattacaa caagcgctcg accatcacct ccagggagat ccagacggcc
     7981 gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg cactaaggcc
     8041 atcaccaagt acaccagcgc taaggatcca ccggtcgccg gcagtggaga gggcagagga
     8101 agtctgctaa catgcggtga cgtcgaggag aatcctggcc cagaattctg cagatatcca
     8161 gcacagtggc ggccgctcga gtctaggcgc gccagcctcg actgtgcctt ctagttgcca
     8221 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     8281 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     8341 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     8401 tgctggggat gcggtgggct cc
//
