LOCUS       sequence-199302-       24136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rosa26 gene targeting vector to induce a Cre-dependent mosaic of 
            cells expressing different membrane localized fluorescent proteins 
            and Notch signalling genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 24136)
  AUTHORS   Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, 
            Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, 
            Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera 
            S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R
  TITLE     Dual ifgMosaic: A Versatile Method for Multispectral and 
            Combinatorial Mosaic Gene-Function Analysis.
  JOURNAL   Cell. 2017 Aug 10;170(4):800-814.e18. doi: 
            10.1016/j.cell.2017.07.031.
  PUBMED    28802047
REFERENCE   2  (bases 1 to 24136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "800-814.e18"
COMMENT     Sequence Label: iMb-Notch-Mosaic (IR99.40)
FEATURES             Location/Qualifiers
     source          1..24136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2219..2235
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        2263..2642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2643..2846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2797..2817
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        2891..2909
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3356..3372
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3382..3400
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3419..4501
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(4508..4573,4574..4611)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     enhancer        4625..5004
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5006..5281
                     /label=chicken beta-actin promoter
     intron          5282..6298
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6222..6244
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6306..6325
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(6359..6392)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(6400..6433)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(6442..6475)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6489..6498
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        6549..7048
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6584..6607)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6981..7000
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        7060..7107
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7126..7929
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7189..7208)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7799..7818
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7967..8191
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    8348..8482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8385..8404)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8439..8458
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(8502..8535)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      8658..8667
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8664..8666,8667..8669,8670..9380)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8709..8730)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8970..8989)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9317..9338
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             9390..9443
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9474..9851
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             9876..9929
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    9989..10213
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(10248..10281)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             10325..11752
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             11762..11815
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             11846..12223
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             12248..12301
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             14666..14689
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    14715..14939
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(14974..15007)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      15130..15139
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             15136..15831
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             15850..15903
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             15934..16311
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             16336..16389
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    18011..18235
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    18287..18334
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(18517..18534)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(18706..18722)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    18741..18965
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    19053..23393
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(23444..23460)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(23493..23511)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(23521..23537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      23679..24134
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     23976..23997
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg
     2221 aggtcgacgg tatcgataag cttcacgtgt tcgaaacgcg ttgacattga ttattgacta
     2281 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     2821 ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact gcttactggc
     2881 ttatcgaaat taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa
     2941 gcttggtacc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     3001 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     3061 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     3121 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     3181 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     3241 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     3301 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     3361 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattgga gctccaccgc
     3421 ggcaggccct ccgagcgtgg tggagccgtt ctgtgagaca gccgggtacg agtcgtgacg
     3481 ctggaagggg caagcgggtg gtgggcagga atgcggtccg ccctgcagca accggagggg
     3541 gagggagaag ggagcggaaa agtctccacc ggacgcggcc atggctcggg gggggggggg
     3601 cagcggagga gcgcttccgg ccgacgtctc gtcgctgatt ggcttctttt cctcccgccg
     3661 tgtgtgaaaa cacaaatggc gtgttttggt tggcgtaagg cgcctgtcag ttaacggcag
     3721 ccggagtgcg cagccgccgg cagcctcgct ctgcccactg ggtggggcgg gaggtaggtg
     3781 gggtgaggcg agctggacgt gcgggcgcgg tcggcctctg gcggggcggg ggaggggagg
     3841 gagggtcagc gaaagtagct cgcgcgcgag cggccgccca ccctcccctt cctctggggg
     3901 agtcgtttta cccgccgccg gccgggcctc gtcgtctgat tggctctcgg ggcccagaaa
     3961 actggccctt gccattggct cgtgttcgtg caagttgagt ccatccgccg gccagcgggg
     4021 gcggcgagga ggcgctccca ggttccggcc ctcccctcgg ccccgcgccg cagagtctgg
     4081 ccgcgcgccc ctgcgcaacg tggcaggaag cgcgcgctgg gggcggggac gggcagtagg
     4141 gctgagcggc tgcggggcgg gtgcaagcac gtttccgact tgagttgcct caagaggggc
     4201 gtgctgagcc agacctccat cgcgcactcc ggggagtgga gggaaggagc gagggctcag
     4261 ttgggctgtt ttggaggcag gaagcacttg ctctcccaaa gtcgctctga gttgttatca
     4321 gtaagggagc tgcagtggag taggcgggga gaaggccgca cccttctccg gaggggggag
     4381 gggagtgttg caataccttt ctgggagttc tctgctgcct cctggcttct gaggaccgcc
     4441 ctgggcctgg gagaatccct tccccctctt ccctcgtgat ctgcaactcc agtctttcta
     4501 gcatctgtag ggcgcagtag tccagggttt ccttgatgat gtcatactta tcctgtccct
     4561 tttttttcca cagctcgcgg ttgaggacaa actcttcgcg gtctttccag tggttaatta
     4621 agtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     4681 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     4741 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     4801 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     4861 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     4921 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     4981 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     5041 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     5101 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
     5161 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     5221 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     5281 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     5341 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     5401 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
     5461 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
     5521 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
     5581 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     5641 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     5701 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     5761 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     5821 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     5881 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     5941 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     6001 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     6061 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     6121 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     6181 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     6241 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     6301 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgagat
     6361 aacttcgtat agtatacctt atacgaagtt atcgagctca taacttcgta taggatactt
     6421 tatacgaagt tataactcga gataacttcg tatagcatac attatacgaa gttataccgg
     6481 cagatctcgc caccatggtg agtgtgatta cagaggctag cttggctgca ggtcgtcgaa
     6541 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     6601 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
     6661 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
     6721 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
     6781 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
     6841 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
     6901 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
     6961 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     7021 ctcctcttcc tcatctccgg gcctttcgac ctgcagcctg ttgacaatta atcatcggca
     7081 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggg atcggccatt
     7141 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     7201 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     7261 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     7321 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     7381 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     7441 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     7501 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     7561 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     7621 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgat
     7681 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     7741 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     7801 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     7861 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     7921 ttcttctgag gggatcaatt ctctagagct cgctgatcag cctcgactgt gccttctagt
     7981 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     8041 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     8101 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     8161 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     8221 tcgactagag cttgcggaac ccttcgtaat cagccatacc acatttgtag aggttttact
     8281 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     8341 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8401 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8461 tgtatcttat catgtctgga tctgacatgg taagtaagct tataacttcg tatagtatac
     8521 cttatacgaa gttatggcgc gcctaattca cagccaccat gctgtgctgc atcagaagaa
     8581 ctaaaccggt tgagaagaat gaagaggccg atcaggagct gcagtcgacg gtaccgcggg
     8641 cccgggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     8701 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     8761 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     8821 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg
     8881 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     8941 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     9001 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     9061 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     9121 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     9181 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     9241 ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc aaagacccca
     9301 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     9361 gcatggacga gctgtacaag ggcagtggag agggcagagg aagtctgcta acatgcggtg
     9421 acgtcgagga gaatcctggc ccagaattct gtgtggatac ctaccgctac atcatgccag
     9481 agccagcgaa gtctgctccc gccccgaaaa agggctccaa gaaggcggtg actaaggcgc
     9541 agaagaaagg cggcaagaag cgcaagcgca gccgcaagga gagctattcc atctatgtgt
     9601 acaaggttct gaagcaggtc caccctgaca ccggcatttc gtccaaggcc atgggcatca
     9661 tgaactcgtt tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc
     9721 attacaacaa gcgctcgacc atcacctcca gggagatcca gacggccgtg cgcctgctgc
     9781 tgcctgggga gttggccaag cacgccgtgt ccgagggcac taaggccatc accaagtaca
     9841 ccagcgctaa ggatccaccg gtcgcctgaa gtggagaggg cagaggaagt ctgctaacat
     9901 gcggtgacgt cgaggagaat cctggcccag aattctgcag atatccagca cagtggcggc
     9961 cgctcgagtc taggcgcgcc agcctcgact gtgccttcta gttgccagcc atctgttgtt
    10021 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
    10081 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
    10141 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
    10201 gtgggctcta tggcttctga ggcggaaaga accagctggg gagatctata acttcgtata
    10261 ggatacttta tacgaagtta tggtaccggc gcgccgatcc atgggttgct gtttctccaa
    10321 gaccatggtg agcaagggag aggaggtcat caaagagttc atgcgcttca aggtgcgcat
    10381 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
    10441 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
    10501 ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga
    10561 catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
    10621 cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
    10681 gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa
    10741 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
    10801 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
    10861 gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa
    10921 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
    10981 gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg
    11041 caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt
    11101 gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
    11161 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
    11221 cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc
    11281 cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
    11341 gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg
    11401 cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat
    11461 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
    11521 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccgct acctggtgga
    11581 gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga
    11641 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
    11701 ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca agggcagtgg
    11761 agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccagaatt
    11821 ctgtgtggat acctaccgct acatcatgcc agagccagcg aagtctgctc ccgccccgaa
    11881 aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg
    11941 cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg tccaccctga
    12001 caccggcatt tcgtccaagg ccatgggcat catgaactcg tttgtgaacg acattttcga
    12061 gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga ccatcacctc
    12121 cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca agcacgccgt
    12181 gtccgagggc actaaggcca tcaccaagta caccagcgct aaggatccac cggtcgccgg
    12241 cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc
    12301 agaattccct gagggcttca aagtgtctga ggccagcaag aagaagcggc gggagcccct
    12361 cggcgaggac tccgtgggcc tcaagcccct gaagaacgct tcagacggtg ccctcatgga
    12421 cgacaaccag aatgagtggg gggacgagga cctggagacc aagaagttcc ggttcgagga
    12481 gcccgtggtt ctgcctgacc tggacgacca gacagaccac cggcagtgga ctcagcagca
    12541 cctggatgcc gctgacctgc gcatgtctgc catggccccc acaccgcccc agggtgaggt
    12601 tgacgccgac tgcatggacg tcaatgtccg cgggcctgat ggcttcaccc cgctcatgat
    12661 cgcctcctgc agcgggggcg gcctggagac gggcaacagc gaggaagagg aggacgcgcc
    12721 ggccgtcatc tccgacttca tctaccaggg cgccagcctg cacaaccaga cagaccgcac
    12781 gggcgagacc gccttgcacc tggccgcccg ctactcacgc tctgatgccg ccaagcgcct
    12841 gctggaggcc agcgcagatg ccaacatcca ggacaacatg ggccgcaccc cgctgcatgc
    12901 ggctgtgtct gccgacgcac aaggtgtctt ccagatcctg atccggaacc gagccacaga
    12961 cctggatgcc cgcatgcatg atggcacgac gccactgatc ctggctgccc gcctggccgt
    13021 ggagggcatg ctggaggacc tcatcaactc acacgccgac gtcaacgccg tagatgacct
    13081 gggcaagtcc gccctgcact gggccgccgc cgtgaacaat gtggatgccg cagttgtgct
    13141 cctgaagaac ggggctaaca aagatatgca gaacaacagg gaggagacac ccctgtttct
    13201 ggccgcccgg gagggcagct acgagaccgc caaggtgctg ctggaccact ttgccaaccg
    13261 ggacatcacg gatcatatgg accgcctgcc gcgcgacatc gcacaggagc gcatgcatca
    13321 cgacatcgtg aggctgctgg acgagtacaa cctggtgcgc agcccgcagc tgcacggagc
    13381 cccgctgggg ggcacgccca ccctgtcgcc cccgctctgc tcgcccaacg gctacctggg
    13441 cagcctcaag cccggcgtgc agggcaagaa ggtccgcaag cccagcagca aaggcctggc
    13501 ctgtggaagc aaggaggcca aggacctcaa ggcacggagg aagaagtccc aggatggcaa
    13561 gggctgcctg ctggacagct ccggcatgct ctcgcccgtg gactccctgg agtcacccca
    13621 tggctacctg tcagacgtgg cctcgccgcc actgctgccc tccccgttcc agcagtctcc
    13681 gtccgtgccc ctcaaccacc tgcctgggat gcccgacacc cacctgggca tcgggcacct
    13741 gaacgtggcg gccaagcccg agatggcggc gctgggtggg ggcggccggc tggcctttga
    13801 gactggccca cctcgtctct cccacctgcc tgtggcctct ggcaccagca ccgtcctggg
    13861 ctccagcagc ggaggggccc tgaatttcac tgtgggcggg tccaccagtt tgaatggtca
    13921 atgcgagtgg ctgtcccggc tgcagagcgg catggtgccg aaccaataca accctctgcg
    13981 ggggagtgtg gcaccaggcc ccctgagcac acaggccccc tccctgcagc atggcatggt
    14041 aggcccgctg cacagtagcc ttgctgccag cgccctgtcc cagatgatga gctaccaggg
    14101 cctgcccagc acccggctgg ccacccagcc tcacctggtg cagacccagc aggtgcagcc
    14161 acaaaactta cagatgcagc agcagaacct gcagccagca aacatccagc agcagcaaag
    14221 cctgcagccg ccaccaccac caccacagcc gcaccttggc gtgagctcag cagccagcgg
    14281 ccacctgggc cggagcttcc tgagtggaga gccgagccag gcagacgtgc agccactggg
    14341 ccccagcagc ctggcggtgc acactattct gccccaggag agccccgccc tgcccacgtc
    14401 gctgccatcc tcgctggtcc cacccgtgac cgcagcccag ttcctgacgc ccccctcgca
    14461 gcacagctac tcctcgcctg tggacaacac ccccagccac cagctacagg tgcctgagca
    14521 ccccttcctg accccttcgc cggagtcgcc cgaccaatgg tcgtcctcgt cgccgcactc
    14581 taatgtgtct gactggtctg agggcgtgtc gtcgcccccg ccctccatgc agtcccagat
    14641 cgcgcgcatc ccggaggcct tcaaggacta caaggatgac gatgacaaat aaggcgcgcc
    14701 ctcgagagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
    14761 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
    14821 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
    14881 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
    14941 ttctgaggcg gaaagaacca gctggggact agtataactt cgtatagcat acattatacg
    15001 aagttatggc gcgcctaatt cacagccacc atgctgtgct gcatcagaag aactaagccg
    15061 gttgagaaga atgaagaggc cgatcaggag ctgcagtcga cggtaccgcg ggcccgggat
    15121 ccaccggtcg ccaccatggt gagcgagctg attaaggaga acatgcacat gaagctctac
    15181 atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc
    15241 tacgagggca cccagaccat gagaatcaag gcggtcgagg gcggccctct ccccttcgcc
    15301 ttcgacatcc tggctaccag cttcatgtac ggcagcaaaa ccttcatcaa ccacacccag
    15361 ggcatccccg acttctttaa gcagtccttc cccgagggct tcacatggga gagagtcacc
    15421 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
    15481 ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag
    15541 aagaaaacac tcggctggga ggcctccacc gagaccctgt accccgctga cggcggcctg
    15601 gaaggcagag ccgacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacttg
    15661 aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat
    15721 gtggacagaa gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac
    15781 gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacag aatgtacaag
    15841 ggcagtggag agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc
    15901 ccagaattct gtgtggatac ctaccgctac atcatgccag agccagcgaa gtctgctccc
    15961 gccccgaaaa agggctccaa gaaggcggtg actaaggcgc agaagaaagg cggcaagaag
    16021 cgcaagcgca gccgcaagga gagctattcc atctatgtgt acaaggttct gaagcaggtc
    16081 caccctgaca ccggcatttc gtccaaggcc atgggcatca tgaactcgtt tgtgaacgac
    16141 attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa gcgctcgacc
    16201 atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga gttggccaag
    16261 cacgccgtgt ccgagggcac taaggccatc accaagtaca ccagcgctaa ggatccaccg
    16321 gtcgccggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat
    16381 cctggcccag aattcccagt aatgccctcc ggttttcctc agtctccacg tacgtccccg
    16441 agggcgcgtc ccaaaacccg gataaccgga gcgctcccca tggactactc ggagggcttg
    16501 tccgcggagg agcggcctgc gcatgctcca tcggcgggga agtttggtga gcggcctcca
    16561 cccaaacgac tcactaggga agctatgcga aactatttaa aagaacgagg ggatcaaaca
    16621 gtgctcattc ttcatgcaaa agtcgcacag aagtcttacg gaaatgaaaa acgatttttt
    16681 tgccctcctc cttgtgtgta tcttatgggc agtggttgga agaaaaaaaa agagcaaatg
    16741 gaacgagatg gatgttctga acaagagtct caaccctgtg cgtttattgg aataggaaac
    16801 agtgaccaag aaatgcagca gctgaacttg gaagggaaga actactgtac agccaaaaca
    16861 ttgtacatat ctgattcaga caagagaaaa catttcatgt tgtctgtaaa gatgttctat
    16921 ggcaacagcg atgacattgg tgtgttcctc agcaagcgga taaaggtcat ctccaaaccc
    16981 tccaaaaaga agcagtcact gaaaaatgct gacttgtgca ttgcttcagg aacgaaggtg
    17041 gcactgttca atcaccttcg gtcccagaca gttagtacca ggtacctgca tgtagaagga
    17101 gggaatttcc acgccagttc acaacagtgg ggagcatttt acatccatct cttggacgac
    17161 gacgagtcgg aaggagagga gttcacagtt agagatggct acatccatta cgggcagact
    17221 gtcaagcttg tgtgctcagt gactggcatg gcactcccaa gattgataat taggaaagtt
    17281 gataagcaga cggcattact ggatgcagac gaccctgtat cacaactcca caaatgtgca
    17341 ttttacctta aggatacaga aagaatgtac ttgtgccttt ctcaagaaag aataatccaa
    17401 tttcaggcca ctccatgtcc aaaagaacaa aataaggaaa tgataaacga tggagcttcc
    17461 tggacaatca ttagcacaga caaggccgag tacacgttct atgagggaat gggccccgtc
    17521 cttgccccag tcacccctgt gcctgtcgta gaaagtcttc agttgaatgg cggcggggac
    17581 gtagcaatgc ttgaacttac aggacaaaac tttactccaa atttaagagt gtggtttggg
    17641 gatgtagaag ccgaaacaat gtacagatgt ggagagagca tgctctgtgt ggtcccagac
    17701 atttctgcat tccgggaagg ttggagatgg gtccgccagc cagtccaggt tccagtgact
    17761 ttggtccgaa atgacggggt catttactcc accagcctta ccttcaccta cacaccagag
    17821 ccagggccga ggccacactg cagcgctgca ggagcgattc tcagagccaa ctccagccaa
    17881 gtgccctcca atgagtcaaa cacaaacagc gaggggaatt acacaaatgc cagcacaaat
    17941 tctaccagtg tcacatcgtc cacagcaacc gtggtgtcct aactctcgag tctaggcgcg
    18001 ccagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
    18061 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
    18121 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
    18181 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
    18241 gaggcggaaa gaaccagctg ggggcggccg cgatcaattc ggtaccgaag ttcctattcc
    18301 gaagttccta ttctctagaa agtataggaa cttcctcgag ggtaccccaa ttccgccccc
    18361 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
    18421 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
    18481 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
    18541 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
    18601 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
    18661 agccaccgag ctcggatcca ctagtccagt gtggtggaat tgggggatcc actagttcta
    18721 gagcggccga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
    18781 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
    18841 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
    18901 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
    18961 tatggcttct gaggcggaaa gaaccagctg gggctcgaga tccactagtt ctagcctcga
    19021 ggctagagcg gccgccgcgg atatcgaatt cgctagaaga tgggcgggag tcttctgggc
    19081 aggcttaaag gctaacctgg tgtgtgggcg ttgtcctgca ggggaattga acaggtgtaa
    19141 aattggaggg acaagacttc ccacagattt tcggttttgt cgggaagttt tttaataggg
    19201 gcaaataagg aaaatgggag gataggtagt catctggggt tttatgcagc aaaactacag
    19261 gttattattg cttgtgatcc gcctcggagt attttccatc gaggtagatt aaagacatgc
    19321 tcacccgagt tttatactct cctgcttgag atccttacta cagtatgaaa ttacagtgtc
    19381 gcgagttaga ctatgtaagc agaattttaa tcatttttaa agagcccagt acttcatatc
    19441 catttctccc gctccttctg cagccttatc aaaaggtatt ttagaacact cattttagcc
    19501 ccattttcat ttattatact ggcttatcca acccctagac agagcattgg cattttccct
    19561 ttcctgatct tagaagtctg atgactcatg aaaccagaca gattagttac atacaccaca
    19621 aatcgaggct gtagctgggg cctcaacact gcagttcttt tataactcct tagtacactt
    19681 tttgttgatc ctttgccttg atccttaatt ttcagtgtct atcacctctc ccgtcaggtg
    19741 gtgttccaca tttgggccta ttctcagtcc agggagtttt acaacaatag atgtattgag
    19801 aatccaacct aaagcttaac tttccactcc catgaatgcc tctctccttt ttctccattt
    19861 ataaactgag ctattaacca ttaatggttt ccaggtggat gtctcctccc ccaatattac
    19921 ctgatgtatc ttacatattg ccaggctgat attttaagac attaaaaggt atatttcatt
    19981 attgagccac atggtattga ttactgctta ctaaaatttt gtcattgtac acatctgtaa
    20041 aaggtggttc cttttggaat gcaaagttca ggtgtttgtt gtctttcctg acctaaggtc
    20101 ttgtgagctt gtattttttc tatttaagca gtgctttctc ttggactggc ttgactcatg
    20161 gcattctaca cgttattgct ggtctaaatg tgattttgcc aagcttcttc aggacctata
    20221 attttgcttg acttgtagcc aaacacaagt aaaatgatta agcaacaaat gtatttgtga
    20281 agcttggttt ttaggttgtt gtgttgtgtg tgcttgtgct ctataataat actatccagg
    20341 ggctggagag gtggctcgga gttcaagagc acagactgct cttccagaag tcctgagttc
    20401 aattcccagc aaccacatgg tggctcacaa ccatctgtaa tgggatctga tgccctcttc
    20461 tggtgtgtct gaagaccaca agtgtattca cattaaataa ataaatcctc cttcttcttc
    20521 tttttttttt ttttaaagag aatactgtct ccagtagaat ttactgaagt aatgaaatac
    20581 tttgtgtttg ttccaatatg gtagccaata atcaaattac tctttaagca ctggaaatgt
    20641 taccaaggaa ctaattttta tttgaagtgt aactgtggac agaggagcca taactgcaga
    20701 cttgtgggat acagaagacc aatgcagact ttaatgtctt ttctcttaca ctaagcaata
    20761 aagaaataaa aattgaactt ctagtatcct atttgtttaa actgctagct ttacttaact
    20821 tttgtgcttc atctatacaa agctgaaagc taagtctgca gccattacta aacatgaaag
    20881 caagtaatga taattttgga tttcaaaaat gtagggccag agtttagcca gccagtggtg
    20941 gtgcttgcct ttatgccttt aatcccagca ctctggaggc agagacaggc agatctctga
    21001 gtttgagccc agcctggtct acacatcaag ttctatctag gatagccagg aatacacaca
    21061 gaaaccctgt tggggagggg ggctctgaga tttcataaaa ttataattga agcattccct
    21121 aatgagccac tatggatgtg gctaaatccg tctacctttc tgatgagatt tgggtattat
    21181 tttttctgtc tctgctgttg gttgggtctt ttgacactgt gggctttctt taaagcctcc
    21241 ttcctgccat gtggtctctt gtttgctact aacttcccat ggcttaaatg gcatggcttt
    21301 ttgccttcta agggcagctg ctgagatttg cagcctgatt tccagggtgg ggttgggaaa
    21361 tctttcaaac actaaaattg tcctttaatt ttttttttaa aaaatgggtt atataataaa
    21421 cctcataaaa tagttatgag gagtgaggtg gactaatatt aaatgagtcc ctcccctata
    21481 aaagagctat taaggctttt tgtcttatac ttaacttttt ttttaaatgt ggtatcttta
    21541 gaaccaaggg tcttagagtt ttagtataca gaaactgttg catcgcttaa tcagattttc
    21601 tagtttcaaa tccagagaat ccaaattctt cacagccaaa gtcaaattaa gaatttctga
    21661 cttttaatgt taatttgctt actgtgaata taaaaatgat agcttttcct gaggcagggt
    21721 ctcactatgt atctctgcct gatctgcaac aagatatgta gactaaagtt ctgcctgctt
    21781 ttgtctcctg aatactaagg ttaaaatgta gtaatacttt tggaacttgc aggtcagatt
    21841 cttttatagg ggacacacta agggagcttg ggtgatagtt ggtaaaatgt gtttcaagtg
    21901 atgaaaactt gaattattat caccgcaacc tactttttaa aaaaaaaagc caggcctgtt
    21961 agagcatgct taagggatcc ctaggacttg ctgagcacac aagagtagtt acttggcagg
    22021 ctcctggtga gagcatattt caaaaaacaa ggcagacaac caagaaacta cagttaaggt
    22081 tacctgtctt taaaccatct gcatatacac agggatatta aaatattcca aataatattt
    22141 cattcaagtt ttcccccatc aaattgggac atggatttct ccggtgaata ggcagagttg
    22201 gaaactaaac aaatgttggt tttgtgattt gtgaaattgt tttcaagtga tagttaaagc
    22261 ccatgagata cagaacaaag ctgctatttc gaggtctctt ggtttatact cagaagcact
    22321 tctttgggtt tccctgcact atcctgatca tgtgctaggc ctaccttagg ctgattgttg
    22381 ttcaaataaa cttaagtttc ctgtcaggtg atgtcatatg atttcatata tcaaggcaaa
    22441 acatgttata tatgttaaac atttgtactt aatgtgaaag ttaggtcttt gtgggtttga
    22501 tttttaattt tcaaaacctg agctaaataa gtcattttta catgtcttac atttggtgga
    22561 attgtataat tgtggtttgc aggcaagact ctctgaccta gtaaccctac ctatagagca
    22621 ctttgctggg tcacaagtct aggagtcaag catttcacct tgaagttgag acgttttgtt
    22681 agtgtatact agtttatatg ttggaggaca tgtttatcca gaagatattc aggactattt
    22741 ttgactgggc taaggaattg attctgatta gcactgttag tgagcattga gtggccttta
    22801 ggcttgaatt ggagtcactt gtatatctca aataatgctg gcctttttta aaaagccctt
    22861 gttctttatc accctgtttt ctacataatt tttgttcaaa gaaatacttg tttggatctc
    22921 cttttgacaa caatagcatg ttttcaagcc atattttttt tccttttttt tttttttttt
    22981 ggtttttcga gacagggttt ctctgtatag ccctggctgt cctggaactc actttgtaga
    23041 ccaggctggc ctcgaactca gaaatccgcc tgcctctgcc tcctgagtgc cgggattaaa
    23101 ggcgtgcacc accacgcctg gctaagttgg atattttgtt atataactat aaccaatact
    23161 aactccactg ggtggatttt taattcagtc agtagtctta agtggtcttt attggccctt
    23221 cattaaaatc tactgttcac tctaacagag gctgttggta ctagtggcac ttaagcaact
    23281 tcctacggat atactagcag attaagggtc agggatagaa actagtctag cgttttgtat
    23341 acctaccagc tttatactac cttgttctga tagaaatatt tcaggacatc tagcttatcg
    23401 ataccgtcga cgttcgaaca cgtggaattc ctgcagcccg ggggatccac tagttctaga
    23461 gcggccgcca ccgcggtgga gctccaattc gccctatagt gagtcgtatt acgcgcgctc
    23521 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
    23581 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
    23641 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
    23701 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
    23761 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
    23821 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    23881 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    23941 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    24001 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    24061 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
    24121 aacgcttaca atttag
//
