LOCUS       sequence-199309-       11076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUDP004 expressing a polycistronic g-RNA array for Cas9 editing 
            targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. 
            pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11076)
  AUTHORS   de Vries ARG, de Groot PA, van den Broek M, Daran JG
  TITLE     CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces 
            pastorianus.
  JOURNAL   Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 
            10.1186/s12934-017-0835-1.
  PUBMED    29207996
REFERENCE   2  (bases 1 to 11076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-12-5"; volume: "16(1):222. doi"; pages: " 
            10.1186/s12934-017-0835-1"
COMMENT     Sequence Label: pUDP044
FEATURES             Location/Qualifiers
     source          1..11076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           11..47
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        68..143
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           148..215
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           232..268
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        289..364
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           369..436
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(478..496)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(join(930..1721,1722..1790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGTRVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1553..1572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1791..1895)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1998..2586
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2487..2506
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             5323..5346
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5350..5370
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5371..9471
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9475..9495
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        join(10414..11076,1..4)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     10950..10973
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 caaaattcac ctgatgagtc cgtgaggacg aaacgagtaa gctcgtcgtg aataatattc
       61 tgagctagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
      121 aaaaagtggc accgagtcgg tgcttttggc cggcatggtc ccagcctcct cgctggcgcc
      181 ggctgggcaa catgcttcgg catggcgaat gggacacagc gcaaaattag gctgatgagt
      241 ccgtgaggac gaaacgagta agctcgtccc taatttgttg tgtatcttgt tttagagcta
      301 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      361 gtgcttttgg ccggcatggt cccagcctcc tcgctggcgc cggctgggca acatgcttcg
      421 gcatggcgaa tgggacacag gccccttttc ctttgtcgat atcatgtaat tagttatgtc
      481 acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca
      541 acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt
      601 tatatttcaa atttttcttt tttttctgta caaacgcgtg tacgcatgta acattatact
      661 gaaaaccttg cttgagaagg ttttgggaca agcttacgtc tcacggatcg tatatgccgt
      721 agcgacaatc taagaactat gcgaggacac gctagcagct gtattcacgt agacggatag
      781 gtatagccag acatcagcag catacttcgg gaaccgtagg ccaccgagcg gccgcgatta
      841 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg ggacccacgc
     1081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgtacccaac
     1681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1921 gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1981 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2041 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2101 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2161 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2221 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2281 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2341 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2401 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2461 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2521 tttccccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2581 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2641 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtgcgga ctatatgtga
     2701 aggcatggct atggcacggc agacattccg ccagatcatc aataggcacg acatggaggc
     2761 ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg
     2821 cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg
     2881 ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc
     2941 tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg
     3001 ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata
     3061 cagttctcac atcacatccg aacataaaca aggatccatg ccacaatctt gggaagaatt
     3121 ggctgctgac aagagagcta gattggctaa gactatccca gacgaatgga aggttcaaac
     3181 tttgccagct gaagactctg ttatcgactt cccaaagaag tctggtatct tgtctgaagc
     3241 tgaattgaag atcactgaag cttctgctgc tgacttggtt tctaagttgg ctgctggtga
     3301 attgacttct gttgaagtta ctttggcttt ctgtaagaga gctgctatcg ctcaacaatt
     3361 gactaactgt gctcacgaat tcttcccaga cgctgctttg gctcaagcta gagaattgga
     3421 cgaatactac gctaagcaca aaagacctgt tggtccattg cacggtttgc caatctcttt
     3481 gaaggaccaa ttgagagtta agggttacga aacttctatg ggttacatct cttggttgaa
     3541 caagtacgac gaaggtgact ctgttttgac tactatgttg agaaaggctg gtgctgtttt
     3601 ctacgttaag acttctgttc cacaaacttt gatggtttgt gaaactgtta acaacatcat
     3661 cggtagaact gttaacccaa gaaacaagaa ctggtcttgt ggtggttctt ctggtggtga
     3721 aggtgctatc gttggtatca gaggtggtgt tatcggtgtt ggtactgaca tcggtggttc
     3781 tatcagagtt ccagctgctt tcaacttctt gtacggttta agaccatctc acggtagatt
     3841 gccatacgct aagatggcta actctatgga aggtcaagaa actgtacact ctgttgttgg
     3901 tccaatcact cactctgttg aagacttgag attgttcact aagtctgttt tgggtcaaga
     3961 accatggaag tacgactcta aggttatccc aatgccatgg agacaatctg aatctgacat
     4021 catcgcttct aagatcaaga acggtggttt gaacatcggt tactacaact tcgacggtaa
     4081 cgttttgcca cacccaccaa tcttgagagg tgttgaaact actgttgctg ctttggctaa
     4141 ggctggtcac actgttactc catggactcc atacaagcac gacttcggtc acgacttgat
     4201 ctctcacatc tacgctgctg acggttctgc tgacgttatg agagacatct ctgcttctgg
     4261 tgaaccagct atcccaaaca tcaaggactt gttgaaccca aacatcaagg ctgttaacat
     4321 gaacgaattg tgggacactc acttgcaaaa gtggaactac caaatggaat acttggaaaa
     4381 gtggagagaa gctgaagaaa aggctggtaa ggaattggac gctatcatcg ctccaatcac
     4441 tccaactgct gctgttagac acgaccaatt cagatactac ggttacgctt ctgttatcaa
     4501 cttgttggac ttcacttctg ttgttgttcc agttactttc gctgacaaga acatcgacaa
     4561 gaagaacgaa tctttcaagg ctgtttctga attggacgct ttggttcaag aagaatacga
     4621 cccagaagct taccacggtg ctccagttgc tgttcaagtt atcggtagaa gattgtctga
     4681 agaaagaact ttggctatcg ctgaagaagt tggtaagttg ttgggtaacg ttgttactcc
     4741 ataactcgag gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt
     4801 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct
     4861 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg
     4921 tatgtgaatg ctggtcgcta tactggagct ctcgagaacc ctcacctttc gagaggacga
     4981 tgcccgtgtc taaatgattc gaccagccta agaatgttca acaatctata atcagtccat
     5041 agtcaacaag agccctcaaa gttgatatcg tttagtgatc tccctcgaga tgagcggagg
     5101 atggttgctt tttggggagc cctaagtggt acgtgcggcg agcgggatgt ttccccatta
     5161 ggcaaaggac gaccgggtaa cccctcgaga aaaaaaattt ttcgccgcta atctggtgtt
     5221 atataaagct ccccctgctc ctggaatttt tttccttgtc aactcacacc ggaaatcaag
     5281 gcatttcatt ctgagtagtt cttaaaaaca taatcaacaa tggattataa agatgacgat
     5341 gacaaacctc caaaaaagaa gagaaaggtc gataagaaat actcaatagg cttagatatc
     5401 ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc gtctaaaaag
     5461 ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat aggggctctt
     5521 ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc tcgtagaagg
     5581 tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa tgagatggcg
     5641 aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga agaagacaag
     5701 aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta tcatgagaaa
     5761 tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctacttataa agcggatttg
     5821 cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt tttgattgag
     5881 ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt ggtacaaacc
     5941 tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc taaagcgatt
     6001 ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca gctccccggt
     6061 gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt gacccctaat
     6121 tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa agatacttac
     6181 gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga tttgtttttg
     6241 gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt aaatactgaa
     6301 ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca tcatcaagac
     6361 ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa agaaatcttt
     6421 tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag ccaagaagaa
     6481 ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga attattggtg
     6541 aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg ctctattacc
     6601 catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga cttttatcca
     6661 tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat tccttattat
     6721 gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa gtctgaagaa
     6781 acaattaccc catggaattt tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt
     6841 attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact accaaaacat
     6901 agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa atatgttact
     6961 gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat tgttgattta
     7021 ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa
     7081 atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa tgcttcatta
     7141 ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga taatgaagaa
     7201 aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga tagggagatg
     7261 attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat gaaacagctt
     7321 aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa tggtattagg
     7381 gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt tgccaatcgc
     7441 aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat tcaaaaagca
     7501 caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc tggtagccct
     7561 gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt caaagtaatg
     7621 gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca gacaactcaa
     7681 aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat caaagaatta
     7741 ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc
     7801 tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt agatattaat
     7861 cgtttaagtg attatgatgt cgatcacatt gttccacaaa gtttccttaa agacgattca
     7921 atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca
     7981 agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa cgccaagtta
     8041 atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt gagtgaactt
     8101 gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac taagcatgtg
     8161 gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa acttattcga
     8221 gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa agatttccaa
     8281 ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta tctaaatgcc
     8341 gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt tgtctatggt
     8401 gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa
     8461 gcaaccgcaa aatatttctt ttactctaat atcatgaact tcttcaaaac agaaattaca
     8521 cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga aactggagaa
     8581 attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc catgccccaa
     8641 gtcaatattg tcaagaaaac agaagtacag acaggcggat tctccaagga gtcaatttta
     8701 ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag actgggatcc aaaaaaatat
     8761 ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa
     8821 gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat tatggaaaga
     8881 agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa ggaagttaaa
     8941 aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa cggtcgtaaa
     9001 cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggctct gccaagcaaa
     9061 tatgtgaatt ttttatattt agctagtcat tatgaaaagt tgaagggtag tccagaagat
     9121 aacgaacaaa aacaattgtt tgtggagcag cataagcatt atttagatga gattattgag
     9181 caaatcagtg aattttctaa gcgtgttatt ttagcagatg ccaatttaga taaagttctt
     9241 agtgcatata acaaacatag agacaaacca atacgtgaac aagcagaaaa tattattcat
     9301 ttatttacgt tgacgaatct tggagctccc gctgctttta aatattttga tacaacaatt
     9361 gatcgtaaac gatatacgtc tacaaaagaa gttttagatg ccactcttat ccatcaatcc
     9421 atcactggtc tttatgaaac acgcattgat ttgagtcagc taggaggtga ccctccaaaa
     9481 aagaagagaa aggtctgagc ggttttgtat aactaaataa tattggaaac taaatacgaa
     9541 tacccaaatt ttttatctaa attttgccga aagattaaaa tctgcagaga tatccgaaac
     9601 aggtaaatgg atgtttcaat ccctgtagtc agtcaggaac ccatattata ttacagtatt
     9661 agtcgccgct taggcacgcc tttaattagc aaaatcaaac cttaagtgca tatgccgtat
     9721 aagggaaact caaagaactg gcatcgcaaa aatgaaaaca tacgttgaaa ctacggcaaa
     9781 ggattggtca gatcgcttca tacagggaaa gttcggcatc aacatctttg gataatatca
     9841 gaatgagaaa gaacagatac gcagtacgtt ttttggtgag ctctttgcac ttctttagtt
     9901 ctttccatca atatcagttt tttaaacttt taggactaaa agtgatgttt aacttcaaaa
     9961 tgtttaaaat tttgttcttc ccgacgttca ttaagaatac taatacactt taataattat
    10021 tttaatattt tgttctaaat aatgactttt aattaaaaaa gataaaatat aaaaacatca
    10081 taataactca ccagaggtta agaacaaaaa aacaaattag atatctgcta atccaatata
    10141 gttaaatcaa tctttccttg gtataatggg tatattacat atatttcaag gaccgacact
    10201 cctaccaaat atctaaaatt taccatatta acataacatg tatataaacg tcaaatcata
    10261 atcagcacta acgtctcacg gatcgtatat gccgtagcga caatctaaga actatgcgag
    10321 gacacgctag ggccaaggca aaaagattcc ttgattacgt aagggagtta gaatcatttt
    10381 gaataaaaaa cacgcttttt cagttcgagt ttatcattat caatactgcc atttcaaaga
    10441 atacgtaaat aattaatagt agtgattttc ctaactttat ttagtcaaaa aattagcctt
    10501 ttaattctgc tgtaacccgt acatgcccaa aatagggggc gggttacaca gaatatataa
    10561 catcgtaggt gtctgggtga acagtttatt cttggcatcc actaaatata atggagcccg
    10621 ctttttaagc tggcatccag aaaaaaaaag aatcccagca ccaaaatatt gttttcttca
    10681 ccaaccatca gttcataggt ccattctctt agcgcaacta cagagaacag gggcacaaac
    10741 aggcaaaaaa cgggcacaac ctcaatggag tgatgcaacc tgcctggagt aaatgatgac
    10801 acaaggcaat tgacccacgc atgtatctat ctcattttct tacaccttct attaccttct
    10861 gctctctctg atttggaaaa agctgaaaaa aaaggttgaa accagttccc tgaaattatt
    10921 cccctacttg actaataagt atataaagac ggtaggtatt gattgtaatt ctgtaaatct
    10981 atttcttaaa cttcttaaat tctactttta tagttagtct tttttttagt tttaaaacac
    11041 caagaactta gtttcgaata aacacacata aacaaa
//
