LOCUS       sequence-19932-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Zhu H, Kavsak P, Abdollah S, Wrana JL, Thomsen GH
  TITLE     A SMAD ubiquitin ligase targets the BMP pathway and affects 
            embryonic pattern formation.
  JOURNAL   Nature. 1999 Aug 12. 400(6745):687-93.
  PUBMED    10458166
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1999 Aug 12. 400(6745):687-93."
COMMENT     Sequence Label: pGEX4T1-Smurf1 C699A
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             40..60
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctggttccgc gtggatcgga ttacgatatc ccaacgaccg aaaacctgta ttttcagggc
       61 gcccatatgg gaggatcccc ggaattcaaa ggcctacgtc gacaaagcaa cgtggtgaca
      121 cggagaggag gctccagcat caagatccgt ctgacagtgt tatgtgccaa gaaccttgca
      181 aagaaagact tcttcaggct ccctgaccct tttgcaaaga ttgtcgtgga tgggtctggg
      241 cagtgccact caaccgacac tgtgaaaaac acattggacc caaagtggaa ccagcactat
      301 gatctatatg ttgggaaaac ggattcgata accattagcg tgtggaacca taagaaaatt
      361 cacaagaaac agggagctgg cttcctgggc tgtgtgcggc tgctctccaa tgccatcagc
      421 agattaaaag ataccggata ccagcgtttg gatctatgca aactaaaccc ctcagatact
      481 gatgcagttc gtggccagat agtggtcagt ttacagacac gagacagaat aggaaccggc
      541 ggctcggtgg tggactgcag aggactgtta gaaaatgaag gaacggtgta tgaagactcc
      601 gggcctggga ggccgctcag ctgcttcatg gaggaaccag ccccttacac agatagcacc
      661 ggtgctgctg ctggaggagg gaattgcagg ttcgtggagt ccccaagtca agatcaaaga
      721 cttcaggcac agcggctt
//
