LOCUS       sequence-199334-       10557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Levy C, Khaled M, Iliopoulos D, Janas MM, Schubert S, Pinner S, Chen
            PH, Li S, Fletcher AL, Yokoyama S, Scott KL, Garraway LA, Song JS, 
            Granter SR, Turley SJ, Fisher DE, Novina CD
  TITLE     Intronic miR-211 assumes the tumor suppressive function of its host 
            gene in melanoma.
  JOURNAL   Mol Cell. 2010 Dec 10. 40(5):841-9.
  PUBMED    21109473
REFERENCE   2  (bases 1 to 10557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2010 Dec 10. 40(5):841-9."
COMMENT     Sequence Label: PLKO-mcherry-luc-puro-Renilla cDNA
FEATURES             Location/Qualifiers
     source          1..10557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     70..87
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    204..225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(240..245,246..263,264..270)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    278..294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     283..305
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     302..318
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     302..318
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     337..357
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        339..357
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        385..611
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             612..792
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    839..964
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1457..1690
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1875..1919
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2094..2604
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             2613..3548
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    3590..3707
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3756..4266
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     regulatory      4275..4284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5940..5999
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     primer_bind     complement(6151..6169)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             6729..6782
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     LTR             7460..7693
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7765..7886
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7802..7821)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7856..7875
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7926..8061
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8081..8100)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8082..8100)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8110..8127)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8110..8126)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8119..8141)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8268..8723
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8355..8374)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8565..8586
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8749..8853
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8854..8922,8923..9714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9072..9091)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9885..10473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10374..10393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
       61 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      121 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      181 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      241 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      301 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
      361 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
      421 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
      481 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
      541 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
      601 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
      661 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
      721 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
      781 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
      841 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
      901 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
      961 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     1021 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     1081 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     1141 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     1201 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     1261 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     1321 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     1381 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     1441 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     1501 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     1561 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     1621 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     1681 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     1741 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     1801 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     1861 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     1921 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     1981 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     2041 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccggggttg
     2101 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     2161 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     2221 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     2281 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     2341 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     2401 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     2461 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     2521 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     2581 cgaatcaccg acctctctcc ccagggaccg gtatgacttc gaaagtttat gatccagaac
     2641 aaaggaaacg gatgataact ggtccgcagt ggtgggccag atgtaaacaa atgaatgttc
     2701 ttgattcatt tattaattat tatgattcag aaaaacatgc agaaaatgct gttatttttt
     2761 tacatggtaa cgcggcctct tcttatttat ggcgacatgt tgtgccacat attgagccag
     2821 tagcgcggtg tattatacca gaccttattg gtatgggcaa atcaggcaaa tctggtaatg
     2881 gttcttatag gttacttgat cattacaaat atcttactgc atggtttgaa cttcttaatt
     2941 taccaaagaa gatcattttt gtcggccatg attggggtgc ttgtttggca tttcattata
     3001 gctatgagca tcaagataag atcaaagcaa tagttcacgc tgaaagtgta gtagatgtga
     3061 ttgaatcatg ggatgaatgg cctgatattg aagaagatat tgcgttgatc aaatctgaag
     3121 aaggagaaaa aatggttttg gagaataact tcttcgtgga aaccatgttg ccatcaaaaa
     3181 tcatgagaaa gttagaacca gaagaatttg cagcatatct tgaaccattc aaagagaaag
     3241 gtgaagttcg tcgtccaaca ttatcatggc ctcgtgaaat cccgttagta aaaggtggta
     3301 aacctgacgt tgtacaaatt gttaggaatt ataatgctta tctacgtgca agtgatgatt
     3361 taccaaaaat gtttattgaa tcggacccag gattcttttc caatgctatt gttgaaggtg
     3421 ccaagaagtt tcctaatact gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag
     3481 atgcacctga tgaaatggga aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg
     3541 aacaataaga attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa
     3601 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     3661 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     3721 gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa
     3781 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
     3841 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
     3901 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
     3961 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
     4021 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
     4081 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     4141 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     4201 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     4261 ccccaggggg atccgccacc atggaagatg ccaagaacat caagaaaggc cctgccccct
     4321 tctaccccct ggaagatggc acagccggcg agcagctgca caaggccatg aagagatacg
     4381 ccctcgtccc tggaacaatc gccttcaccg acgcccacat cgaggtggac atcacctacg
     4441 ccgagtactt cgagatgagc gtgcggctgg ccgaggccat gaaacgctac ggcctgaaca
     4501 ccaaccaccg gatcgtggtg tgcagcgaga acagcctgca gttcttcatg cccgtgctgg
     4561 gcgccctgtt catcggcgtg gccgtggccc ctgccaacga catctacaac gagcgggagc
     4621 tgctgaacag catgggcatc agccagccca ccgtggtgtt cgtgagcaag aagggcctgc
     4681 agaaaatcct gaacgtgcag aagaagctgc ccatcatcca gaaaatcatc atcatggaca
     4741 gcaagaccga ctaccagggc ttccagagca tgtacacctt cgtgaccagc cacctgcccc
     4801 ctggcttcaa cgagtacgac ttcgtgcccg agagcttcga cagggacaag accatcgccc
     4861 tgatcatgaa cagcagcggc agcaccggcc tgcctaaagg cgtggccctg cctcaccgga
     4921 ccgcctgcgt gcggttcagc cacgcccggg accccatctt cggcaaccag atcatccccg
     4981 acaccgccat cctgagcgtg gtgcccttcc accacggctt cggcatgttc accaccctgg
     5041 gctacctgat ctgcggcttc cgggtggtgc tgatgtatcg gttcgaggaa gagctgttcc
     5101 tgcggagcct gcaggactac aagatccaga gcgccctgct ggtgcccacc ctgttcagct
     5161 ttttcgccaa gagcaccctg atcgacaagt acgacctgag caacctgcac gagatcgcca
     5221 gcggcggagc ccccctgagc aaagaagtgg gcgaagccgt cgccaagcgg ttccacctgc
     5281 ccggcatccg gcagggctat ggcctgaccg agaccacaag cgccattctg atcacccccg
     5341 agggcgacga caagcctggc gccgtgggca aggtggtgcc tttcttcgag gccaaggtgg
     5401 tggacctgga caccggcaag accctgggcg tgaaccagcg gggcgagctg tgcgtgaggg
     5461 gccccatgat catgagcggc tacgtgaaca accccgaggc caccaacgcc ctgattgaca
     5521 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
     5581 tcgtggaccg gctgaagagc ctgatcaagt acaagggcta ccaggtggcc ccagccgagc
     5641 tggaaagcat cctgctgcag caccccaaca tcttcgacgc cggggtggcc ggcctgcccg
     5701 acgatgacgc cggcgagctg cctgccgccg tggtggtcct cgaacatggc aaaaccatga
     5761 ccgagaaaga aatcgtggac tacgtggcca gccaggtgac caccgccaag aagctgagag
     5821 gcggcgtcgt gtttgtggac gaggtgccca agggcctgac cggcaagctg gacgcccgga
     5881 agatccggga gatcctgatt aaggccaaga agggcggcaa gatcgccgtg ggctccggcc
     5941 agtgcaccaa ctacgccctg ctgaagctgg ccggcgacgt ggagagcaac cctggccctt
     6001 tcgaaatggt gtccaagggc gaagaggaca acatggccat catcaaagag tttatgcggt
     6061 tcaaggtgca catggaaggc agcgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     6121 aaggcaggcc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggacctc
     6181 tgcccttcgc ctgggacatc ctgagccccc agttcatgta cggcagcaag gcctacgtga
     6241 agcaccccgc cgacatcccc gactacctga agctgtcctt ccccgagggc ttcaagtggg
     6301 agcgggtgat gaacttcgag gacggcgggg tggtgaccgt gacccaggat agctccctgc
     6361 aggacggcga gttcatctac aaggtgaaac tgcggggcac caacttcccc agcgacggcc
     6421 ccgtgatgca gaaaaagaca atgggctggg aggccagcag cgagcggatg taccccgagg
     6481 atggcgccct gaagggcgag atcaagcagc ggctgaagct gaaggacggc ggccactacg
     6541 acgccgaggt gaaaaccacc tacaaggcca aaaagcccgt gcagctgcct ggcgcctaca
     6601 acgtgaacat caagctggat atcaccagcc acaacgagga ctacaccatc gtggagcagt
     6661 acgagcgggc cgagggcaga cacagcaccg gcggcatgga cgagctgtac aagctcagcg
     6721 gcagcggcga gggcagaggc agcctgctga cctgcggcga tgtggaggaa aacccaggcc
     6781 caatgaccga gtacaagcct accgtgaggc tggccacccg ggacgacgtg cccagagccg
     6841 tgaggacact ggccgccgcc ttcgccgact accccgccac ccggcacacc gtggaccccg
     6901 accggcacat cgagcgggtg accgagctgc aggaactgtt cctgaccaga gtgggcctgg
     6961 atatcggcaa agtgtgggtg gccgacgacg gagccgccgt ggccgtgtgg accacccccg
     7021 agtccgtgga ggccggagcc gtgtttgccg agatcggccc caggatggcc gagctgtccg
     7081 gcagcaggct ggccgcccag cagcagatgg aaggcctgct ggcccctcac cggcccaaag
     7141 agcccgcctg gttcctggcc accgtgggcg tgagccccga ccaccagggc aagggcctgg
     7201 gcagcgccgt ggtgctgcca ggcgtggaag ccgccgagag ggccggagtg cccgccttcc
     7261 tggaaaccag cgcccccagg aacctgccct tctacgagcg gctgggcttt accgtgaccg
     7321 ccgacgtgga ggtgccagag ggccccagga cctggtgcat gacccggaag ccaggcgcct
     7381 gagaattggg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7441 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     7501 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7561 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7621 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7681 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7741 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     7801 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7861 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     7921 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7981 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8041 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     8101 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8161 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8221 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     8281 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8341 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8401 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8461 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8521 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8581 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8641 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     8701 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     8761 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8821 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8881 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8941 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9001 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9061 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9121 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9181 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9241 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9301 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9361 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9421 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9481 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9541 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9601 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9661 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9721 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9781 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9841 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9901 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9961 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10021 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10081 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10141 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10201 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10261 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10321 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10381 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10441 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10501 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgat
//
