LOCUS       sequence-199351-        6094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid template for making in-situ hybridization probe for myelin 
            basic protein a (mbpa) gene zf-fj330a07.y1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Brosamle C, Halpern ME
  TITLE     Characterization of myelination in the developing zebrafish.
  JOURNAL   Glia. 2002 Jul;39(1):47-57.
  PUBMED    12112375
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Glia."; 
            date: "2002-07"; volume: "39(1)"; pages: "47-57"
COMMENT     Sequence Label: pZL1-ZF - myelin basic protein a (mbpa)
FEATURES             Location/Qualifiers
     source          1..6094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1925..1943)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1926..1943)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1951..1968)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1951..1967)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1960..1982)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2109..2563
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2196..2215)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2405..2426
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             join(2850..2918,2919..3710)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3068..3087)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    3812..3845
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      3923..4511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4412..4431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4665..4682
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4789..4866
                     /gene="lacI"
                     /label=lacI promoter
     CDS             4867..5949
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4886..4905)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    5962..5983
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5998..6003,6004..6021,6022..6028)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6036..6052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6041..6063
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6060..6076
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6060..6076
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 taatacgact cactataggg aaagctggta cgcctgcagg taccggtccg gaattcccgg
       61 gtcgacccac gcgtccgaga aggtttgcag aacgtctcca gagcggactt tgggttgagc
      121 ggagaaggac atacgtaggc cgtacagact gtagaccact gaacagatca acacctagaa
      181 tggccactgc aagcacctct ggacaaaacc ccttcgggct gggcagaaag aagaaggccc
      241 cgggtgtcct ggatcaaatc agcaggttct tcggaggaga caagaagaga aagggaaagg
      301 gttcatttcg tggtcatcta tcctcctctc cacagagacc ccaccactct tccacccgcc
      361 gtcgtggaga cgtcaaccct gtcgtgcatt tcttcaggag ctttgtgtcc tctcctcgtc
      421 ctaagtcgag gggagaaagc cgatctcgct ctccacccaa acgctggagc accatcttct
      481 gagcccttct ccaagcagga aaacactgag atggaagaga gtgaaatgga cggaaagcaa
      541 aaacttgaga gggaggatgt ctgccctaca gactcaccag tcgcagcgag tttaacagac
      601 taacattggc catcttcgct tcctagatag agatacaatc caagtatctg ttgctacatg
      661 cctgcagggt tacagaagca cgtgttgact gtatgtgtgc aaacttgctg taataattgt
      721 caatggtcag gtgatgcgat acgtcttgta agtctccctt ttaaaattta gctgaagtga
      781 tcaatttcaa catatacaaa gagcaaaata tcatacaaaa ggtttgaaac aattagacag
      841 agtatttctc ttttttttaa atccctgaac caaccagatg aataatttga tcattctgaa
      901 ttggtcttat atgtgtttca caaaatggat tgcttatatg ctctccagca tttgatgtgt
      961 ggcatttatt ctatgttata ctgcctctcc atggttttct tgagatccat gttcaacctc
     1021 atgtgatgtg catttctgta tgtttgtgtt cactgtggtc tttgtgttgc attctatatt
     1081 ggttatttac tttataccag gaatttgtat ataggaatct ttattgagtt ttaattaatg
     1141 caaaaaatat atgatgacca gttaactaca ttaaatttga ttcattttga gaaatgattt
     1201 agctcttaat caggaccatg ccttaaaatg attaaaaaca gactaaaaac acaattttgt
     1261 ggactaggga atagattcta agcatgtatg tggcttggat tgtatgccct cagatgttgc
     1321 actgcagtat gtgtgttaaa accacctgta aatgttgtct gcgtcattac atgtgcaatt
     1381 ttagtgttat tttagaaagg tctcgtaatt accaggggta ggattaatat ttaaataaca
     1441 aacatgcaga aatctaggac aaagagtcag tgggagcata aaggaaaggg tatgaaaaaa
     1501 aggaaggaaa acctgaaagt gcaagatgga taatgtggga aatgctaaat gaggacttct
     1561 gaaagagtaa ggtggagttt attcagctga tttttttttt ctttttctgt gttgtatctc
     1621 atgtgtctta atatcgttca ttgttctgtg tctcatatac tctgcctgta tggactgttt
     1681 ctgaaatagt gctgaataaa cattaggtca aatattctcc ctgtcagcgc tggatttgtc
     1741 tccaaaatgg tgctacactt tctaaaatgc acaggatatt ctgaagtggc caggtcttct
     1801 gatgtaatat ctttaaccct ctctagtaac gataaataaa gaatcagaaa tgtaaaaaaa
     1861 aaaaaagggc ggccgctcta gaggatccaa gcttacgtac gcgtgcatgc gacgtcatag
     1921 ctcttctata gtgtcaccta aattcaattc actggccgtc gttttacaac gtcgtgactg
     1981 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     2041 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     2101 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2161 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     2221 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2281 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2341 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2401 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     2461 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     2521 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     2581 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2641 tcatgccagg tcttggactg gtgagaacgg cttgctcggc agcttcgatg tgtgctggag
     2701 ggagaataaa ggtctaagat gtgcgataga gggaagtcgc attgaattat gtgctgtgta
     2761 gggatcgctg gtatcaaata tgtgtgccca cccctggcat gagacaataa ccctgataaa
     2821 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2881 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2941 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3001 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3061 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3121 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3181 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3241 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3301 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3361 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3421 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3481 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3541 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3601 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3661 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3721 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3781 aggtgaagat cctttttgat aatctcatgc cataacttcg tataatgtat gctatacgaa
     3841 gttatggcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3901 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3961 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4021 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4081 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4141 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4201 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4261 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4321 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4381 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4441 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4501 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4561 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4621 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4681 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     4741 aatgcagagc ttgcaattcg cgcgcgaagg cgaagcggca tttacgttga caccatcgaa
     4801 tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg
     4861 gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag
     4921 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg
     4981 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc
     5041 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa
     5101 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg
     5161 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgg
     5221 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct
     5281 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt
     5341 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt
     5401 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg
     5461 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc
     5521 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt
     5581 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc
     5641 gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac
     5701 agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa
     5761 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg
     5821 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct
     5881 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     5941 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     6001 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     6061 aggaaacagc tatgaccatg attacgccaa gctc
//
