LOCUS       sequence-199377-        8113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soluble BLInCR effector that is recruited to 'localizer' sites upon 
            blue light illumination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pPHR-mCherry-hPMLIII
FEATURES             Location/Qualifiers
     source          1..8113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        48..351
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        352..555
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     506..526
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             612..2105
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     regulatory      2124..2133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2130..2837
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2275..2293)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2543..2562
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4888..5009
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4925..4944)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4979..4998
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5016..5471)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5153..5174)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5365..5384
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5498..5602
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5604..5961
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5604..5795
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5627..5647)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5812..5947
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5996..6790
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5996..6790
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6050..6069)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6660..6679
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6978..6997)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7022..7069
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7398..7986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7887..7906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
       61 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      121 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      181 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      241 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      301 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      361 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      421 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      481 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      541 tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg gactcagatc
      601 tcgaggccac catgaagatg gacaaaaaga ctatagtttg gtttagaaga gacctaagga
      661 ttgaggataa tcctgcatta gcagcagctg ctcacgaagg atctgttttt cctgtcttca
      721 tttggtgtcc tgaagaagaa ggacagtttt atcctggaag agcttcaaga tggtggatga
      781 aacaatcact tgctcactta tctcaatcct tgaaggctct tggatctgac ctcactttaa
      841 tcaaaaccca caacacgatt tcagcgatct tggattgtat ccgcgttacc ggtgctacaa
      901 aagtcgtctt taaccacctc tatgatcctg tttcgttagt tcgggaccat accgtaaagg
      961 agaagctggt ggaacgtggg atctctgtgc aaagctacaa tggagatcta ttgtatgaac
     1021 cgtgggagat atactgcgaa aagggcaaac cttttacgag tttcaattct tactggaaga
     1081 aatgcttaga tatgtcgatt gaatccgtta tgcttcctcc tccttggcgg ttgatgccaa
     1141 taactgcagc ggctgaagcg atttgggcgt gttcgattga agaactaggg ctggagaatg
     1201 aggccgagaa accgagcaat gcgttgttaa ctagagcttg gtctccagga tggagcaatg
     1261 ctgataagtt actaaatgag ttcatcgaga agcagttgat agattatgca aagaacagca
     1321 agaaagttgt tgggaattct acttcactac tttctccgta tctccatttc ggggaaataa
     1381 gcgtcagaca cgttttccag tgtgcccgga tgaaacaaat tatatgggca agagataaga
     1441 acagtgaagg agaagaaagt gcagatcttt ttcttagggg aatcggttta agagagtatt
     1501 ctcggtatat atgtttcaac ttcccgttta ctcacgagca atcgttgttg agtcatcttc
     1561 ggtttttccc ttgggatgct gatgttgata agttcaaggc ctggagacaa ggcaggaccg
     1621 gttatccgtt ggtggatgcc ggaatgagag agctttgggc taccggatgg atgcataaca
     1681 gaataagagt gattgtttca agctttgctg tgaagtttct tctccttcca tggaaatggg
     1741 gaatgaagta tttctgggat acacttttgg atgctgattt ggaatgtgac atccttggct
     1801 ggcagtatat ctctgggagt atccccgatg gccacgagct tgatcgcttg gacaatcccg
     1861 cgttacaagg cgccaaatat gacccagaag gtgagtacat aaggcaatgg cttcccgagc
     1921 ttgcgagatt gccaactgaa tggatccatc atccatggga cgctccttta accgtactca
     1981 aagcttctgg tgtggaactc ggaacaaact atgcgaaacc cattgtagac atcgacacag
     2041 ctcgtgagct actagctaaa gctatttcaa gaacccgtga agcacagatc atgatcggag
     2101 cagcagcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg
     2161 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     2221 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     2281 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     2341 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     2401 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     2461 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     2521 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     2581 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     2641 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     2701 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     2761 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     2821 tggacgagct gtacaagggt atggagcctg cacccgcccg atctccgagg ccccagcagg
     2881 accccgcccg gccccaggag cccaccatgc ctccccccga gaccccctct gaaggccgcc
     2941 agcccagccc cagccccagc cctacagagc gagcccccgc ttcggaggag gagttccagt
     3001 ttctgcgctg ccagcaatgc caggcggaag ccaagtgccc gaagctgctg ccttgtctgc
     3061 acacgctgtg ctcaggatgc ctggaggcgt cgggcatgca gtgccccatc tgccaggcgc
     3121 cctggcccct aggtgcagac acacccgccc tggataacgt ctttttcgag agtctgcagc
     3181 ggcgcctgtc ggtgtaccgg cagattgtgg atgcgcaggc tgtgtgcacc cgctgcaaag
     3241 agtcggccga cttctggtgc tttgagtgcg agcagctcct ctgcgccaag tgcttcgagg
     3301 cacaccagtg gttcctcaag cacgaggccc ggcccctagc agagctgcgc aaccagtcgg
     3361 tgcgtgagtt cctggacggc acccgcaaga ccaacaacat cttctgctcc aaccccaacc
     3421 accgcacccc tacgctgacc agcatctact gccgaggatg ttccaagccg ctgtgctgct
     3481 cgtgcgcgct ccttgacagc agccacagtg agctcaagtg cgacatcagc gcagagatcc
     3541 agcagcgaca ggaggagctg gacgccatga cgcaggcgct gcaggagcag gatagtgcct
     3601 ttggcgcggt tcacgcgcag atgcacgcgg ccgtcggcca gctgggccgc gcgcgtgccg
     3661 agaccgagga gctgatccgc gagcgcgtgc gccaggtggt agctcacgtg cgggctcagg
     3721 agcgcgagct gctggaggct gtggacgcgc ggtaccagcg cgactacgag gagatggcca
     3781 gtcggctggg ccgcctggat gctgtgctgc agcgcatccg cacgggcagc gcgctggtgc
     3841 agaggatgaa gtgctacgcc tcggaccagg aggtgctgga catgcacggt ttcctgcgcc
     3901 aggcgctctg ccgcctgcgc caggaggagc cccagagcct gcaagctgcc gtgcgcaccg
     3961 atggcttcga cgagttcaag gtgcgcctgc aggacctcag ctcttgcatc acccagggga
     4021 aagatgcagc tgtatccaag aaagccagcc cagaggctgc cagcactccc agggacccta
     4081 ttgacgttga cctgcccgag gaggcagaga gagtgaaggc ccaggttcag gccctggggc
     4141 tggctgaagc ccagcctatg gctgtggtac agtcagtgcc cggggcacac cccgtgccag
     4201 tgtacgcctt ctccatcaaa ggcccttcct atggagagga tgtctccaat acaacgacag
     4261 cccagaagag gaagtgcagc cagacccagt gccccaggaa ggtcatcaag atggagtctg
     4321 aggaggggaa ggaggcaagg ttggctcgga gctccccgga gcagcccagg cccagcacct
     4381 ccaaggcagt ctcaccaccc cacctggatg gaccgcctag ccccaggagc cccgtcatag
     4441 gaagtgaggt cttcctgccc aacagcaacc acgtggccag tggcgccggg gaggcagagg
     4501 aacgcgttgt ggtgatcagc agctcggaag actcagatgc cgaaaactcg gtctcttcca
     4561 gccctcagtc tgaggttctg tattggaaag tgcatggagc ccatggagac cgccgagcca
     4621 cagtcctcgc cagcccactc ctcgccagcc cactcctcgc cagcccactc ctcgccagtc
     4681 cagtctctgc tgagagcaca aggagcctcc agcctgccct gtggcacata ccacccccca
     4741 gcttggcctc cccaccagcc cgctgagcgg ccgcgactct agatcataat cagccatacc
     4801 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4861 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4921 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4981 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt
     5041 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     5101 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     5161 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     5221 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     5281 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     5341 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     5401 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     5461 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5521 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5581 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     5641 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5701 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5761 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5821 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5881 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5941 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     6001 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6061 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6121 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     6181 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6241 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6301 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6361 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6421 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6481 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     6541 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6601 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6661 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6721 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     6781 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     6841 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6901 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6961 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     7021 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     7081 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     7141 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     7201 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     7261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     7561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
     8101 tagttattaa tag
//
