LOCUS       sequence-199384-        9550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SSX1 promoter drives mKate2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN342 (FuGW-SSX1p-mKate2)
FEATURES             Location/Qualifiers
     source          1..9550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        29..133
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(134..202,203..994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(352..371)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1165..1753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1654..1673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1907..1924
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2041..2062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2077..2082,2083..2100,2101..2107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2115..2131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2120..2142
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2139..2155
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2139..2155
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2204..2325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2215..2234)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2269..2288
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2455..2829)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2848..2895)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(2943..3272)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2957..3092
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3011..3030)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3257..3277
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3286..3714)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3396..3417)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3608..3627
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3760..3984
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3973..3990
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             4016..4196
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     4701..4717
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4702..4718
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4721..5309
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4827..4838
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5236..5256
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      6175..6184
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6181..6894
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    6994..7111
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7409..7453)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7638..7871
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8364..8489
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8536..8716
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8734..8932)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8759..8779)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8934..9313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9485..9504
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
       61 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      121 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      181 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      241 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      301 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      361 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      421 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      481 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      541 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      661 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
      721 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
      781 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      841 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      961 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     1021 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1081 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1741 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1801 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1921 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1981 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     2041 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2101 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2161 ttacgccaag ctctagctag aggtcgacgg tatacagaca tgataagata cattgatgag
     2221 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     2281 gctattgctt tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa
     2341 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     2401 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtagca cgtgtcagtc
     2461 ctgctcctcg gccacgaagt gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg
     2521 cccccacggc tgctcgccga tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt
     2581 ggacacgacc tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc
     2641 cagggtgttg tccggcacca cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc
     2701 ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg gagaacccga gccggtcggt
     2761 ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa
     2821 cttggccatg gtttagttcc tcaccttgtc gtattatact atgccgatat actatgccga
     2881 tgattaattg tcaacacgtg ctgatcagat ccgaaaatgg atatacaagc tcccgggagc
     2941 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     3001 gcagaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     3061 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     3121 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     3181 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     3241 cctggggact ttccacaccc taactgacac acattccaca gaattaattc gcgttaaatt
     3301 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     3361 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     3421 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     3481 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     3541 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     3601 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     3661 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat
     3721 accccctaga gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc
     3781 cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc
     3841 ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa
     3901 ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag
     3961 atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttaaacg ggccctgcta
     4021 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     4081 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     4141 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     4201 cagtccggat gcagctctcg ggccatgtga tgaaatgcta ggcggctgtc aaacctccac
     4261 tctaatactt ctctctccgg gtcatccatc ccatgcaggc tcacagggtg taacaagcgg
     4321 gtgttctctc cttcattggc ttcttctacc ttctcttgct caactggtac tagcttgtag
     4381 caccatccaa aggtcagtgg atatctgatc cctggccctg gtgtgtagtt ctgccaatca
     4441 gggaagtagc cttgtgtgtg gtagatccac agatcaagga tatcttgtct tcgttgggag
     4501 tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc
     4561 ttgtaagtca ttggtcttaa aggtacctga ggtgtgactg gaaaacccac ctcctcctcc
     4621 tcttgtgctt ctagccaggc acaatcagca ttggtagctg ctgtattgct acttgtgatt
     4681 gctccatgtt tttctaggtc tcgaggtcga cggtatcgat gcggggaggc ggcccaaagg
     4741 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     4801 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     4861 ccgcgcagaa tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc
     4921 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     4981 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     5041 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     5101 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     5161 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     5221 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     5281 tcacaaattt tgtaatccag aggttgatta tcgataagct tgatatcgcg gccgccctgc
     5341 agggtagcca gatcatggct cactgcaacc tcgtactcct gggctcaagc tatcctccta
     5401 cctcagcctc ctgagtaacg gactacaggc acaccacccc acctcgctaa ttttatttat
     5461 tttttttgta gagaaaagag acagggtatt gctctgttgc ccagggtggg gtgcagtggc
     5521 atgatcatgg ctcactgcaa cctctgcctc ccaggttcaa gtgatcctcc agctgtggcc
     5581 tccctaagtg ctgggattac aaccgtgagc cgccgcaccg gcccaaattt cttacgtcac
     5641 tacagagttc ctaggaaaaa atcccatacc tgaaaaagat agaaactgac aggaaggatt
     5701 tgagatgatg acctgcttca tatacactcc ttattaaaac tggataacaa tgcaccaccg
     5761 aggaggtggg gagggatagg aaaaatgaaa agagaaaatc agcgcatgcg tactctgatt
     5821 tgggaagact ccaaagagaa aatcagagca tgcgtactct gaacttggag tagccaatcc
     5881 caggggatgc tttaggcggg aaagtcagag tttctgcctc cattttgaga aggttctgtc
     5941 cctagagcct agactgatag accccacatc agcttggctt gtcccgccta ctgttctgac
     6001 ttctgattgg ccagatggag ttcactaact gccctgattg gtccatcatc ctggagcaat
     6061 gacattgcag aatattttct cctcctccag ccacactttg tcaccaactg ctgccaactc
     6121 gccaccactg ctgccgacct cgcaaccact gctttgtctc tggcgcgcca gctcgccacc
     6181 atggtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct gtacatggag
     6241 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     6301 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     6361 atcctggcta ccagcttcat gtacggcagc aaaaccttca tcaaccacac ccagggcatc
     6421 cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     6481 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     6541 tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
     6601 acactcggct gggaggcctc caccgagatg ctgtaccccg ctgacggcgg cctggaaggc
     6661 agaagcgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttgaagacc
     6721 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac
     6781 agaagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg
     6841 gctgtggcca gatactgcga cctccctagc aaactggggc acaaacttaa ttgataaacc
     6901 ggttgcagtc ccgggtaagc gggactctgg ggttcgaaat gaccgaccaa gcattaacca
     6961 aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt
     7021 ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact
     7081 gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca tttgtctgca
     7141 gaattggcgc acgcagtgcc gatccgttca ctaatcgaat ggatctgtct ctgtctctct
     7201 ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg
     7261 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
     7321 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
     7381 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
     7441 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
     7501 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
     7561 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
     7621 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
     7681 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
     7741 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
     7801 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
     7861 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
     7921 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
     7981 aattgtccct catatctcct cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg
     8041 gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
     8101 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
     8161 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat
     8221 gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata
     8281 tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat
     8341 ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg
     8401 ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct
     8461 tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc
     8521 ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg agggatctct
     8581 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     8641 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     8701 ctaaccagag agacccagta caggcaaaac gcgctgctta tatagacctc ccaccgtaca
     8761 cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
     8821 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
     8881 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
     8941 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
     9001 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
     9061 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
     9121 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
     9181 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
     9241 aactccatat atgggctatg aactaatgac cccgtaattg attactatta ataactagtc
     9301 aataatcaat gtcaacgcgt atatctggcc cgtacatcgc gaagcagcgc aaaacgccta
     9361 accctaagca gattcttcat gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt
     9421 ttgctcgcgc actactcagc gacctccaac acacaagcag ggagcagata ctggcttaac
     9481 tatgcggcat cagagcagat tgtactgaga gtgcaccata ggggatcggg agatctcccg
     9541 atccgtcgac
//
