LOCUS       sequence-199390-        9114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic promoter YBX1 drives mKate2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN351 (FuGW-S(YBX1)p-mKate2 )
FEATURES             Location/Qualifiers
     source          1..9114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..118
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     103..119
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    122..710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             228..239
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     637..657
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      1140..1149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1146..1859
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1959..2076
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2374..2418)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2603..2836
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3329..3454
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3501..3681
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3699..3897)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3724..3744)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3899..4278
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4450..4469
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4544..4648
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4649..4717,4718..5509)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4867..4886)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5680..6268
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6169..6188
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6422..6439
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6556..6577
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6592..6597,6598..6615,6616..6622)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6630..6646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6635..6657
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6654..6670
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6654..6670
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6719..6840
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6730..6749)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6784..6803
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6970..7344)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7363..7410)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7458..7787)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7472..7607
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7526..7545)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7772..7792
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7801..8229)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7911..7932)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8123..8142
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8275..8499
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8488..8505
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8531..8711
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct
       61 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgaggtcg acggtatcga
      121 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
      181 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
      241 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
      301 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
      361 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      421 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      481 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      541 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      601 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      661 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
      721 ttgatatcgc ggccgccctg cacctgcagg ctgattggcc aaagcctgat tggccaaatc
      781 ctgattggcc aagacctgat tggccaaact ctgattggcc aagtctctga ttggccaagg
      841 agctgattgg ccaatccctg atggccaacg aattcctgcc ctactgacac tgcctgccgc
      901 tgattggcca aagcctgatt ggccaaatcc tgattggcca agacctgatt ggccaaactc
      961 tgattggcca agtcctgatt ggccaagagc tgattggcca atccctgatt ggccaacgaa
     1021 tatccgcggc gcgccagacg ctagcggggg gctataaaag ggggtggggg cgttcgtcct
     1081 cactctagat ctgcgatcta agtaagcttg gcattccggt actgttggta aaccagctcg
     1141 ccaccatggt gtctaagggc gaagagctga ttaaggagaa catgcacatg aagctgtaca
     1201 tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct
     1261 acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct
     1321 tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac cacacccagg
     1381 gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca
     1441 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     1501 tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga
     1561 agaaaacact cggctgggag gcctccaccg agatgctgta ccccgctgac ggcggcctgg
     1621 aaggcagaag cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttga
     1681 agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg
     1741 tggacagaag actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg
     1801 aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaaa cttaattgat
     1861 aaaccggttg cagtcccggg taagcgggac tctggggttc gaaatgaccg accaagcatt
     1921 aaccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
     1981 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
     2041 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
     2101 ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat cgaatggatc tgtctctgtc
     2161 tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag
     2221 gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac
     2281 agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata attttatata
     2341 ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa
     2401 taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa
     2461 gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg
     2521 ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt
     2581 tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag
     2641 gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc
     2701 ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc
     2761 ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc
     2821 aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc
     2881 cttggtgggt gctactccta atggttcaat ttttactact ttatatttat ataattcact
     2941 tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc gcttgctgtg
     3001 cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt
     3061 ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa
     3121 gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt
     3181 ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata
     3241 ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc
     3301 gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc
     3361 ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc
     3421 gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca
     3481 agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga
     3541 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     3601 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     3661 ctggtctaac cagagagacc cagtacaggc aaaacgcgct gcttatatag acctcccacc
     3721 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     3781 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     3841 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
     3901 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     3961 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     4021 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     4081 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     4141 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     4201 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     4261 tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac
     4321 gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt
     4381 aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc agatactggc
     4441 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga tcgggagatc
     4501 tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4561 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4621 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4681 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4741 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4801 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4861 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4921 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4981 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5041 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5101 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5161 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5221 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5281 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5341 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5401 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5461 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5521 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5581 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5641 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5701 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5761 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5821 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5881 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5941 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6001 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6061 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6121 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6181 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6241 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6301 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6361 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6421 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     6481 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     6541 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     6601 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     6661 cagctatgac catgattacg ccaagctcta gctagaggtc gacggtatac agacatgata
     6721 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     6781 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     6841 ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     6901 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     6961 tagcacgtgt cagtcctgct cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg
     7021 cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc
     7081 gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg
     7141 cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa
     7201 cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa
     7261 cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg
     7321 aacggcactg gtcaacttgg ccatggttta gttcctcacc ttgtcgtatt atactatgcc
     7381 gatatactat gccgatgatt aattgtcaac acgtgctgat cagatccgaa aatggatata
     7441 caagctcccg ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
     7501 tggaatagct cagaggcaga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     7561 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     7621 ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     7681 gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt
     7741 ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt ccacagaatt
     7801 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     7861 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     7921 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     7981 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     8041 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     8101 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     8161 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     8221 agggcgcgtg gggatacccc ctagagcccc agctggttct ttccgcctca gaagccatag
     8281 agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct
     8341 gccccacccc accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct
     8401 cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg
     8461 aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat cagcgggttt
     8521 aaacgggccc tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     8581 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     8641 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     8701 cagagagacc cagtacagtc cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg
     8761 ctgtcaaacc tccactctaa tacttctctc tccgggtcat ccatcccatg caggctcaca
     8821 gggtgtaaca agcgggtgtt ctctccttca ttggcttctt ctaccttctc ttgctcaact
     8881 ggtactagct tgtagcacca tccaaaggtc agtggatatc tgatccctgg ccctggtgtg
     8941 tagttctgcc aatcagggaa gtagccttgt gtgtggtaga tccacagatc aaggatatct
     9001 tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta
     9061 agatctacag ctgccttgta agtcattggt cttaaaggta cctgaggtgt gact
//
