LOCUS       sequence-199393-        9124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic promoter SP1 drives mKate2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN377 (FuGW-S(SP1)p-mKate2 )
FEATURES             Location/Qualifiers
     source          1..9124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..118
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     103..119
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    122..710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             228..239
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     637..657
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      1150..1159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1156..1869
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1969..2086
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2384..2428)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2613..2846
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3339..3464
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3511..3691
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3709..3907)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3734..3754)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3909..4288
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4460..4479
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4554..4658
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4659..4727,4728..5519)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4877..4896)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5690..6278
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6179..6198
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6432..6449
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6566..6587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6602..6607,6608..6625,6626..6632)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6640..6656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6645..6667
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6664..6680
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6664..6680
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6729..6850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6740..6759)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6794..6813
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6980..7354)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7373..7420)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7468..7797)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7482..7617
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7536..7555)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7782..7802
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7811..8239)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7921..7942)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8133..8152
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8285..8509
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8498..8515
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8541..8721
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct
       61 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgaggtcg acggtatcga
      121 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
      181 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
      241 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
      301 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
      361 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      421 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      481 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      541 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      601 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      661 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
      721 ttgatatcgc ggccgccctg cacctgcagg tagggtgggc gtggcagccg gggcggggca
      781 tctagggcgg ggccgacggg gggcggggcc acttagggtg ggcgtggcgt ccggggcggg
      841 gcgagtaggg cggggcctcc ggggggcggg gcccgaattc tgccctactg acactgcctg
      901 ccgtagggtg ggcgtggcag ccggggcggg gcatctaggg cggggccgac ggggggcggg
      961 gccacttagg gtgggcgtgg cgtccggggc ggggcgagta gggcggggcc tccggggggc
     1021 ggggcccgaa tatccgcggc gcgccagacg ctagcggggg gctataaaag ggggtggggg
     1081 cgttcgtcct cactctagat ctgcgatcta agtaagcttg gcattccggt actgttggta
     1141 aaccagctcg ccaccatggt gtctaagggc gaagagctga ttaaggagaa catgcacatg
     1201 aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa
     1261 ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc
     1321 cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac
     1381 cacacccagg gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag
     1441 agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag
     1501 gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct
     1561 gtgatgcaga agaaaacact cggctgggag gcctccaccg agatgctgta ccccgctgac
     1621 ggcggcctgg aaggcagaag cgacatggcc ctgaagctcg tgggcggggg ccacctgatc
     1681 tgcaacttga agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc
     1741 gtctactatg tggacagaag actggaaaga atcaaggagg ccgacaaaga gacctacgtc
     1801 gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaaa
     1861 cttaattgat aaaccggttg cagtcccggg taagcgggac tctggggttc gaaatgaccg
     1921 accaagcatt aaccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat
     1981 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     2041 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
     2101 tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat cgaatggatc
     2161 tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg
     2221 gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta
     2281 tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata
     2341 attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat
     2401 ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg
     2461 agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat
     2521 tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc
     2581 aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt
     2641 ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat
     2701 gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata
     2761 attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc
     2821 ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc
     2881 ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact ttatatttat
     2941 ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc
     3001 gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
     3061 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
     3121 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
     3181 ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg
     3241 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
     3301 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
     3361 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
     3421 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
     3481 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
     3541 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     3601 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     3661 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaacgcgct gcttatatag
     3721 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     3781 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     3841 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     3901 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     3961 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     4021 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     4081 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     4141 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     4201 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     4261 tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc
     4321 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
     4381 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc
     4441 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga
     4501 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4561 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4621 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4681 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4741 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4801 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4861 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4921 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4981 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5041 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5101 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5161 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5221 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5281 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5341 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5401 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5461 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5521 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5581 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5641 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5701 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5761 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5821 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5881 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5941 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6001 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6061 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6121 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6181 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6241 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6301 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6361 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6421 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6481 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6541 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6601 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6661 acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc gacggtatac
     6721 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6781 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     6841 taaacaagtt ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     6901 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     6961 cgaaatctcg tagcacgtgt cagtcctgct cctcggccac gaagtgcacg cagttgccgg
     7021 ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg
     7081 gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt
     7141 ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg
     7201 cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt
     7261 cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg
     7321 tgagcaccgg aacggcactg gtcaacttgg ccatggttta gttcctcacc ttgtcgtatt
     7381 atactatgcc gatatactat gccgatgatt aattgtcaac acgtgctgat cagatccgaa
     7441 aatggatata caagctcccg ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     7501 tcactacttc tggaatagct cagaggcaga ggcggcctcg gcctctgcat aaataaaaaa
     7561 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     7621 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     7681 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     7741 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     7801 ccacagaatt aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     7861 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     7921 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     7981 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     8041 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     8101 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     8161 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     8221 gcgccgctac agggcgcgtg gggatacccc ctagagcccc agctggttct ttccgcctca
     8281 gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc
     8341 ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag acaatgcgat
     8401 gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa
     8461 ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat
     8521 cagcgggttt aaacgggccc tgctagagat tttccacact gactaaaagg gtctgaggga
     8581 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     8641 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     8701 ctggtctaac cagagagacc cagtacagtc cggatgcagc tctcgggcca tgtgatgaaa
     8761 tgctaggcgg ctgtcaaacc tccactctaa tacttctctc tccgggtcat ccatcccatg
     8821 caggctcaca gggtgtaaca agcgggtgtt ctctccttca ttggcttctt ctaccttctc
     8881 ttgctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc tgatccctgg
     8941 ccctggtgtg tagttctgcc aatcagggaa gtagccttgt gtgtggtaga tccacagatc
     9001 aaggatatct tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa
     9061 aaagtggcta agatctacag ctgccttgta agtcattggt cttaaaggta cctgaggtgt
     9121 gact
//
