LOCUS       sequence-199395-        9116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic promoter E2F1 drives mKate2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN379 (FuGW-S(E2F1)p-mKate2)
FEATURES             Location/Qualifiers
     source          1..9116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..118
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     103..119
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    122..710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             228..239
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     637..657
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      1142..1151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1148..1861
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1961..2078
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2376..2420)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2605..2838
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3331..3456
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3503..3683
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3701..3899)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3726..3746)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3901..4280
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4452..4471
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4546..4650
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4651..4719,4720..5511)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4869..4888)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5682..6270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6171..6190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6424..6441
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6558..6579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6594..6599,6600..6617,6618..6624)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6632..6648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6637..6659
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6656..6672
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6656..6672
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6721..6842
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6732..6751)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6786..6805
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6972..7346)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7365..7412)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7460..7789)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7474..7609
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7528..7547)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7774..7794
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7803..8231)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7913..7934)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8125..8144
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8277..8501
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8490..8507
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8533..8713
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct
       61 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgaggtcg acggtatcga
      121 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
      181 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
      241 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
      301 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
      361 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      421 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      481 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      541 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      601 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      661 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
      721 ttgatatcgc ggccgccctg cacctgcagg tacgcgcgaa aactgagcgc gccaaaatcg
      781 ctgagcgcga aacggacggg gcgggaagac ttacgcgcga aaactggtcg cgccaaagag
      841 gctgagcgcg aaacgtccgg ggcgggaagc gaattctgcc ctactgacac tgcctgccgt
      901 acgcgcgaaa actgagcgcg ccaaaatcgc tgagcgcgaa acggacgggg cgggaagact
      961 tacgcgcgaa aactggtcgc gccaaagagg ctgagcgcga aacgtccggg gcgggaagcg
     1021 aatatccgcg gcgcgccaga cgctagcggg gggctataaa agggggtggg ggcgttcgtc
     1081 ctcactctag atctgcgatc taagtaagct tggcattccg gtactgttgg taaaccagct
     1141 cgccaccatg gtgtctaagg gcgaagagct gattaaggag aacatgcaca tgaagctgta
     1201 catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc
     1261 ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc
     1321 cttcgacatc ctggctacca gcttcatgta cggcagcaaa accttcatca accacaccca
     1381 gggcatcccc gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac
     1441 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
     1501 cctcatctac aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca
     1561 gaagaaaaca ctcggctggg aggcctccac cgagatgctg taccccgctg acggcggcct
     1621 ggaaggcaga agcgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt
     1681 gaagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta
     1741 tgtggacaga agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca
     1801 cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca aacttaattg
     1861 ataaaccggt tgcagtcccg ggtaagcggg actctggggt tcgaaatgac cgaccaagca
     1921 ttaaccaaac tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa
     1981 tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc
     2041 ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt
     2101 gtctgcagaa ttggcgcacg cagtgccgat ccgttcacta atcgaatgga tctgtctctg
     2161 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
     2221 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
     2281 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
     2341 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
     2401 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
     2461 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
     2521 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
     2581 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
     2641 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
     2701 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
     2761 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
     2821 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     2881 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     2941 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg
     3001 tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc
     3061 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct
     3121 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc
     3181 ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca
     3241 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca
     3301 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta
     3361 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     3421 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt
     3481 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg
     3541 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     3601 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     3661 atctggtcta accagagaga cccagtacag gcaaaacgcg ctgcttatat agacctccca
     3721 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     3781 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     3841 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     3901 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     3961 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     4021 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     4081 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     4141 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     4201 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     4261 actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa
     4321 acgcctaacc ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc
     4381 ttaaattttg ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg
     4441 gcttaactat gcggcatcag agcagattgt actgagagtg caccataggg gatcgggaga
     4501 tctcccgatc cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4561 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4621 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4681 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4741 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4801 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4861 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4921 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4981 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5041 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5101 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5161 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5221 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5281 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5341 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5401 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5461 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5521 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5581 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5641 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5701 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5761 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5821 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5881 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5941 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6001 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6061 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6121 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6181 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6241 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6301 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6361 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6421 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6481 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     6541 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     6601 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     6661 aacagctatg accatgatta cgccaagctc tagctagagg tcgacggtat acagacatga
     6721 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     6781 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     6841 ttggggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg
     6901 tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct
     6961 cgtagcacgt gtcagtcctg ctcctcggcc acgaagtgca cgcagttgcc ggccgggtcg
     7021 cgcagggcga actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag
     7081 gcgtcccgga agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg
     7141 cgcacccaca cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg
     7201 aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag
     7261 aacccgagcc ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc
     7321 ggaacggcac tggtcaactt ggccatggtt tagttcctca ccttgtcgta ttatactatg
     7381 ccgatatact atgccgatga ttaattgtca acacgtgctg atcagatccg aaaatggata
     7441 tacaagctcc cgggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     7501 tctggaatag ctcagaggca gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     7561 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     7621 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     7681 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     7741 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagaa
     7801 ttaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     7861 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     7921 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     7981 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     8041 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     8101 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     8161 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     8221 acagggcgcg tggggatacc ccctagagcc ccagctggtt ctttccgcct cagaagccat
     8281 agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc
     8341 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
     8401 ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg
     8461 ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgggt
     8521 ttaaacgggc cctgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
     8581 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
     8641 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
     8701 accagagaga cccagtacag tccggatgca gctctcgggc catgtgatga aatgctaggc
     8761 ggctgtcaaa cctccactct aatacttctc tctccgggtc atccatccca tgcaggctca
     8821 cagggtgtaa caagcgggtg ttctctcctt cattggcttc ttctaccttc tcttgctcaa
     8881 ctggtactag cttgtagcac catccaaagg tcagtggata tctgatccct ggccctggtg
     8941 tgtagttctg ccaatcaggg aagtagcctt gtgtgtggta gatccacaga tcaaggatat
     9001 cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc
     9061 taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt gtgact
//
