LOCUS       sequence-199397-        9132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic promoter NFY drives mKate2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN381 (FuGW-S(NFY)p-mKate2)
FEATURES             Location/Qualifiers
     source          1..9132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..118
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     103..119
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    122..710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             228..239
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     637..657
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      1158..1167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1164..1877
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1977..2094
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2392..2436)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2621..2854
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3347..3472
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3519..3699
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3717..3915)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3742..3762)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3917..4296
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4468..4487
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4562..4666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4667..4735,4736..5527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4885..4904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5698..6286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6187..6206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6440..6457
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6574..6595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6610..6615,6616..6633,6634..6640)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6648..6664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6653..6675
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6672..6688
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6672..6688
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6737..6858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6748..6767)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6802..6821
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6988..7362)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7381..7428)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7476..7805)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7490..7625
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7544..7563)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7790..7810
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7819..8247)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7929..7950)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8141..8160
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8293..8517
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8506..8523
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8549..8729
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct
       61 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgaggtcg acggtatcga
      121 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
      181 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
      241 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
      301 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
      361 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      421 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      481 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      541 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      601 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      661 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
      721 ttgatatcgc ggccgccctg cacctgcagg tcagccaatc agagagcgag ccaatcagaa
      781 tcccagccaa tcagagactg ggccaatcag aaacttcagc caatcagagg tcgagccaat
      841 cagagagcca gccaatcaga tcctgggcca atcagaacga attctgccct actgacactg
      901 cctgccgtca gccaatcaga gagcgagcca atcagaatcc cagccaatca gagactgggc
      961 caatcagaaa cttcagccaa tcagaggtcg agccaatcag agagccagcc aatcagatcc
     1021 tgggccaatc agaacgaata tccgcggcgc gccagacgct agcggggggc tataaaaggg
     1081 ggtgggggcg ttcgtcctca ctctagatct gcgatctaag taagcttggc attccggtac
     1141 tgttggtaaa ccagctcgcc accatggtgt ctaagggcga agagctgatt aaggagaaca
     1201 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg
     1261 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     1321 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaaacct
     1381 tcatcaacca cacccagggc atccccgact tctttaagca gtccttccct gagggcttca
     1441 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     1501 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca
     1561 acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag atgctgtacc
     1621 ccgctgacgg cggcctggaa ggcagaagcg acatggccct gaagctcgtg ggcgggggcc
     1681 acctgatctg caacttgaag accacataca gatccaagaa acccgctaag aacctcaaga
     1741 tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc gacaaagaga
     1801 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg
     1861 ggcacaaact taattgataa accggttgca gtcccgggta agcgggactc tggggttcga
     1921 aatgaccgac caagcattaa ccaaactgga tctctgctgt ccctgtaata aacccgaaaa
     1981 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
     2041 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg
     2101 atgaatactg ccatttgtct gcagaattgg cgcacgcagt gccgatccgt tcactaatcg
     2161 aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg
     2221 tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct
     2281 attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat
     2341 tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc
     2401 ccatttatct aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc
     2461 aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt
     2521 cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc
     2581 agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag
     2641 gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg
     2701 caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc
     2761 agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc
     2821 tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg
     2881 caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt
     2941 atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat
     3001 cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta
     3061 aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg
     3121 tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca
     3181 gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta
     3241 gctccctgct tgcccatact atatgtttta atttatattt tttctttccc cctggcctta
     3301 accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc
     3361 catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc
     3421 cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg
     3481 gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga
     3541 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     3601 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     3661 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aacgcgctgc
     3721 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     3781 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     3841 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     3901 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     3961 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     4021 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     4081 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     4141 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     4201 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     4261 ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcccgtacat
     4321 cgcgaagcag cgcaaaacgc ctaaccctaa gcagattctt catgcaattg tcggtcaagc
     4381 cttgccttgt tgtagcttaa attttgctcg cgcactactc agcgacctcc aacacacaag
     4441 cagggagcag atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     4501 ataggggatc gggagatctc ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt
     4561 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4621 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4681 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4741 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4801 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4861 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4921 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4981 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5041 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5101 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5161 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5221 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5281 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5341 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5401 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5461 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5521 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5581 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5641 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5701 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5761 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5821 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5881 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5941 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6001 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6061 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6121 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6181 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6241 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6301 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6361 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6421 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6481 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     6541 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     6601 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     6661 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga
     6721 cggtatacag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     6781 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     6841 agctgcaata aacaagttgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     6901 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     6961 ggtggaatcg aaatctcgta gcacgtgtca gtcctgctcc tcggccacga agtgcacgca
     7021 gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt
     7081 catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta
     7141 cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc
     7201 ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc
     7261 cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc
     7321 gcgcgcggtg agcaccggaa cggcactggt caacttggcc atggtttagt tcctcacctt
     7381 gtcgtattat actatgccga tatactatgc cgatgattaa ttgtcaacac gtgctgatca
     7441 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     7501 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     7561 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     7621 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     7681 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     7741 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     7801 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     7861 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     7921 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     7981 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     8041 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     8101 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     8161 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     8221 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     8281 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     8341 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     8401 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     8461 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     8521 aggctgatca gcgggtttaa acgggccctg ctagagattt tccacactga ctaaaagggt
     8581 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
     8641 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
     8701 gctcagatct ggtctaacca gagagaccca gtacagtccg gatgcagctc tcgggccatg
     8761 tgatgaaatg ctaggcggct gtcaaacctc cactctaata cttctctctc cgggtcatcc
     8821 atcccatgca ggctcacagg gtgtaacaag cgggtgttct ctccttcatt ggcttcttct
     8881 accttctctt gctcaactgg tactagcttg tagcaccatc caaaggtcag tggatatctg
     8941 atccctggcc ctggtgtgta gttctgccaa tcagggaagt agccttgtgt gtggtagatc
     9001 cacagatcaa ggatatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
     9061 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc
     9121 tgaggtgtga ct
//
