LOCUS       sequence-199399-       15207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9 fused to human HDAC3 with R265P mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15207)
  AUTHORS   Kwon DY, Zhao YT, Lamonica JM, Zhou Z
  TITLE     Locus-specific histone deacetylation using a synthetic 
            CRISPR-Cas9-based HDAC.
  JOURNAL   Nat Commun. 2017 May 12;8:15315. doi: 10.1038/ncomms15315.
  PUBMED    28497787
REFERENCE   2  (bases 1 to 15207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-12"; volume: "8:15315. doi"; pages: " 
            10.1038/ncomms15315"
COMMENT     Sequence Label: dCas-hHDAC3-R265P
FEATURES             Location/Qualifiers
     source          1..15207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1195..1242
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(1243..1266,1267..1269,1270..3825,3826..3828,
                     3829..5343)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5398..5418
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6736..6789
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6790..7188
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7213..7801
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7266..7286)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7684..7695)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7804..7820)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7805..7821)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8326..8506
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8532..8549)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8538..8762
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8808..9236
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8895..8914)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9105..9126
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9245..9265)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9250..9579
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9430..9565
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9492..9511
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9627..9674
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9693..10067
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10197..10318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10234..10253)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10288..10307
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10367..10383)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10367..10383)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10380..10402)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10391..10407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10415..10421,10422..10439,10440..10445))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10460..10481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10598..10615)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10769..11357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10849..10868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11528..12319,12320..12388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12151..12170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12389..12493)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12568..12587)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12759..13138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13140..13338
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13293..13313
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             13356..13536
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    13583..13708
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    14201..14434
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             14619..14663
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    14961..15078
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgac gtacggccac catgaaaagg
     1201 ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa aggacaagaa gtacagcatc
     1261 ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg
     1321 cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg
     1381 atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc
     1441 gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc
     1501 aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg
     1561 gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc
     1621 taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac
     1681 aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac
     1741 ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag
     1801 ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac
     1861 gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc
     1921 cagctgcccg gcgagaagaa gaatggcctg ttcggcaacc tgattgccct gagcctgggc
     1981 ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc
     2041 aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc
     2101 gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga
     2161 gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag
     2221 caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac
     2281 aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc
     2341 agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag
     2401 gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac
     2461 ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa
     2521 gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc
     2581 atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga
     2641 aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc
     2701 gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg
     2761 ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg
     2821 aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc
     2881 atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac
     2941 tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc
     3001 aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg
     3061 gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag
     3121 gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg
     3181 atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc
     3241 aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc
     3301 ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac
     3361 atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg
     3421 gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc
     3481 gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac
     3541 cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc
     3601 atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag
     3661 aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa
     3721 ctggacatca accggctgtc cgactacgat gtggaccaca tcgtgcctca gagctttctg
     3781 aaggacgact ccatcgacaa caaggtgctg accagaagcg acaaggcccg gggcaagagc
     3841 gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg
     3901 aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc
     3961 ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc
     4021 acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac
     4081 aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg
     4141 aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc
     4201 tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag
     4261 ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag
     4321 gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag
     4381 accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc
     4441 gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg
     4501 agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa
     4561 gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac
     4621 cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc
     4681 aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc
     4741 atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac
     4801 aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa
     4861 aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc
     4921 ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc
     4981 tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac
     5041 gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg
     5101 gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag
     5161 aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt
     5221 gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg
     5281 atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggc
     5341 gacagcgctg gaggaggtgg aagcggagga ggaggaagcg gaggaggagg tagcggacct
     5401 aagaaaaaga ggaaggtggc ggccgctgga tccatggcca agaccgtggc ctatttctac
     5461 gaccccgacg tgggcaactt ccactacgga gctggacacc ctatgaagcc tcatcgcctg
     5521 gcattgaccc atagcctggt cctgcattac ggtctctata agaagatgat cgtcttcaag
     5581 ccataccagg cctcccagca tgacatgtgc cgcttccact ccgaggacta cattgacttc
     5641 ctgcagagag tcagccccac caatatgcaa ggcttcacca agagtcttaa tgccttcaac
     5701 gtaggcgatg actgcccagt gtttcccggg ctctttgagt tctgctcgcg ttacacaggc
     5761 gcatctctgc aaggagcaac ccagctgaac aacaagatct gtgatattgc cattaactgg
     5821 gctggtggtc tgcaccatgc caagaagttt gaggcctctg gcttctgcta tgtcaacgac
     5881 attgtgattg gcatcctgga gctgctcaag taccaccctc gggtgctcta cattgacatt
     5941 gacatccacc atggtgacgg ggttcaagaa gctttctacc tcactgaccg ggtcatgacg
     6001 gtgtccttcc acaaatacgg aaattacttc ttccctggca caggtgacat gtatgaagtc
     6061 ggggcagaga gtggccgcta ctactgtctg aacgtgcccc tgcgggatgg cattgatgac
     6121 cagagttaca agcacctttt ccagccggtt atcaaccagg tagtggactt ctaccaaccc
     6181 acgtgcattg tgctccagtg tggagctgac tctctgggct gtgatccatt gggctgcttt
     6241 aacctcagca tccgagggca tggggaatgc gttgaatatg tcaagagctt caatatccct
     6301 ctactcgtgc tgggtggtgg tggttatact gtccgaaatg ttgcccgctg ctggacatat
     6361 gagacatcgc tgctggtaga agaggccatt agtgaggagc ttccctatag tgaatacttc
     6421 gagtactttg ccccagactt cacacttcat ccagatgtca gcacccgcat cgagaatcag
     6481 aactcacgcc agtatctgga ccagatccgc cagacaatct ttgaaaacct gaagatgctg
     6541 aaccatgcac ctagtgtcca gattcatgac gtgcctgcag acctcctgac ctatgacagg
     6601 actgatgagg ctgatgcaga ggagaggggt cctgaggaga actatagcag gccagaggca
     6661 cccaatgagt tctatgatgg agaccatgac aatgacaagg aaagcgatgt ggagatttaa
     6721 tgtacaggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat
     6781 cctggcccaa tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
     6841 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
     6901 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
     6961 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
     7021 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
     7081 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
     7141 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaaga attcgatatc
     7201 aagcttatcg gtaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7261 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7321 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7381 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7441 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7501 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7561 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7621 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7681 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7741 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7801 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     7861 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     7921 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     7981 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     8041 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     8101 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     8161 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     8221 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     8281 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     8341 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8401 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8461 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     8521 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     8581 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     8641 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     8701 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     8761 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     8821 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8881 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8941 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     9001 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     9061 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     9121 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     9181 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     9241 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     9301 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     9361 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     9421 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9481 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     9541 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     9601 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     9661 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     9721 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     9781 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     9841 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     9901 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     9961 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
    10021 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
    10081 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
    10141 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
    10201 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    10261 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    10321 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    10381 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    10441 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    10501 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    10561 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    10621 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    10681 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    10741 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    10801 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    10861 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10921 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    10981 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    11041 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    11101 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    11161 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    11221 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    11281 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    11341 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    11401 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    11461 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    11521 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    11581 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    11641 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    11701 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    11761 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    11821 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    11881 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    11941 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    12001 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    12061 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    12121 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    12181 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    12241 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    12301 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    12361 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    12421 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    12481 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    12541 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    12601 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
    12661 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
    12721 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
    12781 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
    12841 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
    12901 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
    12961 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
    13021 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
    13081 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
    13141 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
    13201 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
    13261 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
    13321 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
    13381 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
    13441 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
    13501 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
    13561 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
    13621 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
    13681 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
    13741 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
    13801 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
    13861 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
    13921 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
    13981 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
    14041 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
    14101 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
    14161 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
    14221 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
    14281 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
    14341 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
    14401 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
    14461 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
    14521 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
    14581 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
    14641 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
    14701 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
    14761 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
    14821 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
    14881 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
    14941 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
    15001 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
    15061 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
    15121 agctagctgc aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc
    15181 ttctaggtct tgaaaggagt gggaatt
//
