LOCUS       sequence-199400-        9113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic promoter HIF1A drives mKate2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN382 (FuGW-S(HIF1A)p-mKate2 )
FEATURES             Location/Qualifiers
     source          1..9113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..118
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     103..119
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    122..710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             228..239
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     637..657
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      1139..1148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1145..1858
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1958..2075
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2373..2417)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2602..2835
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3328..3453
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3500..3680
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3698..3896)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3723..3743)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3898..4277
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4449..4468
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4543..4647
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4648..4716,4717..5508)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4866..4885)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5679..6267
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6168..6187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6421..6438
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6555..6576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6591..6596,6597..6614,6615..6621)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6629..6645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6634..6656
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6653..6669
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6653..6669
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6718..6839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6729..6748)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6783..6802
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6969..7343)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7362..7409)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7457..7786)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7471..7606
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7525..7544)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7771..7791
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7800..8228)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7910..7931)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8122..8141
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8274..8498
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8487..8504
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8530..8710
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct
       61 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgaggtcg acggtatcga
      121 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
      181 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
      241 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
      301 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
      361 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      421 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      481 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      541 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      601 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      661 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
      721 ttgatatcgc ggccgccctg cacctgcagg agccgcacgt gacagcagcc gcacgtgaca
      781 tccacccacg tgcgaccacc cacgtgcact agccgcacgt gaccacagcc gcacgtgacg
      841 agcacccacg tgctcccacc cacgtgccga attctgccct actgacactg cctgccgagc
      901 cgcacgtgac agcagccgca cgtgacatcc acccacgtgc gaccacccac gtgcactagc
      961 cgcacgtgac catcagccgc acgtgacgag cacccacgtg ctcccaccca cgtgccgaat
     1021 atccgcggcg cgccagacgc tagcgggggg ctataaaagg gggtgggggc gttcgtcctc
     1081 actctagatc tgcgatctaa gtaagcttgg cattccggta ctgttggtaa accagctcgc
     1141 caccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga agctgtacat
     1201 ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta
     1261 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt
     1321 cgacatcctg gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc acacccaggg
     1381 catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga gagtcaccac
     1441 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct
     1501 catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa
     1561 gaaaacactc ggctgggagg cctccaccga gatgctgtac cccgctgacg gcggcctgga
     1621 aggcagaagc gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttgaa
     1681 gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt
     1741 ggacagaaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga
     1801 ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac ttaattgata
     1861 aaccggttgc agtcccgggt aagcgggact ctggggttcg aaatgaccga ccaagcatta
     1921 accaaactgg atctctgctg tccctgtaat aaacccgaaa attttgaatt tttgtaattt
     1981 gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg
     2041 cactgtaccc cccaatcccc ccttttcttt taaaattgtg gatgaatact gccatttgtc
     2101 tgcagaattg gcgcacgcag tgccgatccg ttcactaatc gaatggatct gtctctgtct
     2161 ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg
     2221 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
     2281 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
     2341 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
     2401 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
     2461 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
     2521 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
     2581 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
     2641 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
     2701 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
     2761 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
     2821 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     2881 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     2941 ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg cttgctgtgc
     3001 ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
     3061 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
     3121 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
     3181 tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac
     3241 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
     3301 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
     3361 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
     3421 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
     3481 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
     3541 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     3601 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     3661 tggtctaacc agagagaccc agtacaggca aaacgcgctg cttatataga cctcccaccg
     3721 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
     3781 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     3841 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
     3901 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     3961 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     4021 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     4081 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     4141 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     4201 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     4261 agtcaataat caatgtcaac gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg
     4321 cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta
     4381 aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca gatactggct
     4441 taactatgcg gcatcagagc agattgtact gagagtgcac cataggggat cgggagatct
     4501 cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4561 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4621 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4681 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4741 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4801 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4861 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4921 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4981 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5041 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5101 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5161 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5221 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5281 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5341 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5401 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5461 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5521 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5581 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5641 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5701 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5761 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5821 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5881 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5941 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6001 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6061 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6421 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     6481 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     6541 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     6601 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     6661 agctatgacc atgattacgc caagctctag ctagaggtcg acggtataca gacatgataa
     6721 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     6781 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttg
     6841 gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc
     6901 cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt
     6961 agcacgtgtc agtcctgctc ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc
     7021 agggcgaact cccgccccca cggctgctcg ccgatctcgg tcatggccgg cccggaggcg
     7081 tcccggaagt tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc
     7141 acccacaccc aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac
     7201 agggtcacgt cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac
     7261 ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga
     7321 acggcactgg tcaacttggc catggtttag ttcctcacct tgtcgtatta tactatgccg
     7381 atatactatg ccgatgatta attgtcaaca cgtgctgatc agatccgaaa atggatatac
     7441 aagctcccgg gagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     7501 ggaatagctc agaggcagag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     7561 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     7621 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     7681 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     7741 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagaatta
     7801 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     7861 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     7921 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     7981 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     8041 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     8101 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     8161 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     8221 gggcgcgtgg ggataccccc tagagcccca gctggttctt tccgcctcag aagccataga
     8281 gcccaccgca tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg
     8341 ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc
     8401 attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga
     8461 ggggcaaaca acagatggct ggcaactaga aggcacagtc gaggctgatc agcgggttta
     8521 aacgggccct gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     8581 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     8641 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     8701 agagagaccc agtacagtcc ggatgcagct ctcgggccat gtgatgaaat gctaggcggc
     8761 tgtcaaacct ccactctaat acttctctct ccgggtcatc catcccatgc aggctcacag
     8821 ggtgtaacaa gcgggtgttc tctccttcat tggcttcttc taccttctct tgctcaactg
     8881 gtactagctt gtagcaccat ccaaaggtca gtggatatct gatccctggc cctggtgtgt
     8941 agttctgcca atcagggaag tagccttgtg tgtggtagat ccacagatca aggatatctt
     9001 gtcttcgttg ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa
     9061 gatctacagc tgccttgtaa gtcattggtc ttaaaggtac ctgaggtgtg act
//
