LOCUS       sequence-199409-        8962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic promoter cMYC drives mKate2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN466 (FuGW-S(cMYC)p-mKate2)
FEATURES             Location/Qualifiers
     source          1..8962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    14..602
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             120..131
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     529..549
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      880..889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             886..1599
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1699..1816
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2114..2158)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2343..2576
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3069..3194
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3241..3421
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3439..3637)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3464..3484)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3639..4018
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4190..4209
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4284..4388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4389..4457,4458..5249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4607..4626)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5420..6008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5909..5928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6162..6179
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6296..6317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6332..6337,6338..6355,6356..6362)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6370..6386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6375..6397
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6394..6410
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6394..6410
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6459..6580
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6470..6489)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6524..6543
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6710..7084)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7103..7150)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7198..7527)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7212..7347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7266..7285)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7512..7532
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7541..7969)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7651..7672)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7863..7882
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8015..8239
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8228..8245
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8271..8451
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     join(8956..8962,1..10)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     join(8957..8962,1..11)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgacggtatc gatgcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg
       61 cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga
      121 ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag
      181 gcgagcagcc aaggaaagga cgatgatttc cccgacaaca ccacggaatt gtcagtgccc
      241 aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca
      301 atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa
      361 ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc
      421 cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc
      481 atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa
      541 ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga
      601 ttatcgataa gcttgatatc gcggccgccc tgcacctgca gtccgccacg tgcgcgacag
      661 tccgccacgt gcgcgactgg tccgccacgt gcgcgaccgt ccgccacgtg cgcgaccggt
      721 ccgccacgtg cgcgaccgtc cgccacgtgc gcgacgtgtc cgccacgtgc gcgccagacg
      781 ctagcggggg gctataaaag ggggtggggg cgttcgtcct cactctagat ctgcgatcta
      841 agtaagcttg gcattccggt actgttggta aaccagctcg ccaccatggt gtctaagggc
      901 gaagagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac
      961 caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
     1021 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc
     1081 ttcatgtacg gcagcaaaac cttcatcaac cacacccagg gcatccccga cttctttaag
     1141 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
     1201 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
     1261 agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag
     1321 gcctccaccg agatgctgta ccccgctgac ggcggcctgg aaggcagaag cgacatggcc
     1381 ctgaagctcg tgggcggggg ccacctgatc tgcaacttga agaccacata cagatccaag
     1441 aaacccgcta agaacctcaa gatgcccggc gtctactatg tggacagaag actggaaaga
     1501 atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt ggccagatac
     1561 tgcgacctcc ctagcaaact ggggcacaaa cttaattgat aaaccggttg cagtcccggg
     1621 taagcgggac tctggggttc gaaatgaccg accaagcatt aaccaaactg gatctctgct
     1681 gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt
     1741 ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc
     1801 cccttttctt ttaaaattgt ggatgaatac tgccatttgt ctgcagaatt ggcgcacgca
     1861 gtgccgatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt
     1921 ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg
     1981 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
     2041 ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa tttgttatgt
     2101 taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt
     2161 cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt
     2221 ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc
     2281 aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc
     2341 ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct
     2401 tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat
     2461 tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg
     2521 acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc
     2581 ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta
     2641 atggttcaat ttttactact ttatatttat ataattcact tctccaattg tccctcatat
     2701 ctcctcctcc aggtctgaag atcagcggcc gcttgctgtg cggtggtctt acttttgttt
     2761 tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc
     2821 acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga tcctgtctga
     2881 agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc taacaggcca
     2941 ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt taatttatat
     3001 tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat tctcccccgc
     3061 ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt
     3121 ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc
     3181 ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt cgggcgccac
     3241 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     3301 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
     3361 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     3421 cagtacaggc aaaacgcgct gcttatatag acctcccacc gtacacgcct accgcccatt
     3481 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     3541 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     3601 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     3661 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     3721 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     3781 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     3841 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     3901 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     3961 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
     4021 cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc
     4081 ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac
     4141 tcagcgacct ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag
     4201 cagattgtac tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag
     4261 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4321 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4381 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4441 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4501 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4561 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4621 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4681 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4741 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4801 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4861 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4921 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4981 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5041 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5101 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5161 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5221 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5281 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5341 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5401 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5461 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5521 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5581 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5641 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5701 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5761 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5821 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5881 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5941 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6001 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6061 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6121 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6181 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6241 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     6301 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     6361 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     6421 ccaagctcta gctagaggtc gacggtatac agacatgata agatacattg atgagtttgg
     6481 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     6541 tgctttattt gtaaccatta taagctgcaa taaacaagtt ggggtgggcg aagaactcca
     6601 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     6661 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tagcacgtgt cagtcctgct
     6721 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     6781 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     6841 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     6901 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     6961 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     7021 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     7081 ccatggttta gttcctcacc ttgtcgtatt atactatgcc gatatactat gccgatgatt
     7141 aattgtcaac acgtgctgat cagatccgaa aatggatata caagctcccg ggagcttttt
     7201 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggcaga
     7261 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     7321 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     7381 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     7441 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     7501 ggactttcca caccctaact gacacacatt ccacagaatt aattcgcgtt aaatttttgt
     7561 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     7621 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     7681 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     7741 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     7801 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     7861 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     7921 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtg gggatacccc
     7981 ctagagcccc agctggttct ttccgcctca gaagccatag agcccaccgc atccccagca
     8041 tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
     8101 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
     8161 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
     8221 tggcaactag aaggcacagt cgaggctgat cagcgggttt aaacgggccc tgctagagat
     8281 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     8341 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     8401 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc
     8461 cggatgcagc tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa
     8521 tacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt
     8581 ctctccttca ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca
     8641 tccaaaggtc agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa
     8701 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat
     8761 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     8821 agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg
     8881 tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc
     8941 atgtttttct aggtctcgag gt
//
